Purine and Pyrimidine Metabolism in Man VIII

Front Matter....Pages i-xxi
Diagnosis and Treatment of Inborn Errors of Purine and Pyrimidine Metabolism: An Overview....Pages 1-6
Determination of Dihydropyrimidine Dehydrogenase (DPD) in Fibroblasts of a DPD Deficient Pediatric Patient and Family Members using a Polyclonal Antibody to Human DPD....Pages 7-10
The Effect of Partial Hepatectomy on Blood Purine Levels in Rats and Patients....Pages 11-14
Abnormal Purine and Pyrimidine Metabolism in Inherited Superactivity of PRPP Synthetase....Pages 15-18
Accelerated Purine Base Salvage — A Possible Cause of Elevated Nucleotide Pool in the Erythrocytes of Patients with Uraemia....Pages 19-22
Quantitation of TK1 mRNA in Patients with Chronic Lymphatic Leukemia....Pages 23-26
Effect of Hypouricemic Agents on Serum Carbohydrate-Deficient Transferrin in Gouty Patients....Pages 27-30
Renal Hemodynamic in Familial Nephropathy Associated with Hyperuricemia (FNAH)....Pages 31-34
Renal Urate Hypoexcretion in Polynesian Women is not as Severe as in United Kingdom (UK) Women with Familial Juvenile Hyperuricaemic Nephropathy (FJHN)....Pages 35-38
Renal Clearances of Purine Bases and Oxypurinol During Glucose Infusion....Pages 39-42
 In Vitro and In Vivo Study on the Conversion of Allopurinol and Pyrazinamide....Pages 43-46
Free Oxypurines in Plasma and Urine of Gout Patients Before and After a Purine-Free Diet....Pages 47-52
Analysis of the Genotypes for Aldehyde Dehydrogenase 2 in Japanese Patients with Primary Gout....Pages 53-56
Hypouricemia and an Increased Clearance of Uric Acid are Observed in Liver Diseases?....Pages 57-60
The Influence of Allopurinol on Renal Deterioration in Familial Nepropathy Associated with Hyperuricemia (FNAH)....Pages 61-64
Ultrasound Imaging and Colour Doppler Studies in Familial Nephropathy Associated with Hyperuricemia (FNAH)....Pages 65-68
Purine Metabolism in Female Patients with Primary Gout....Pages 69-72
Familial Juvenile Hyperuricaemic Nephropathy in Adolescents....Pages 73-76
Correlation of Serum Uric Acid, Cholesterol and Triglyceride Levels....Pages 77-78
Serum Concentration of Lp(a) in Patients with Primary Gout....Pages 79-82
Study on Lipoprotein Lipase and Hepatic Triglyceride Lipase Activities in Patients with Gout....Pages 83-86
Benzbromarone and Fenofibrate are Lipid Lowering and Uricosuric: A Possible Key to Metabolic Syndrome?....Pages 87-90
The Normal Range of Serum Urate Levels and of Fractional Urate Excretion....Pages 91-93
Therapy Related Disturbances in Nucleotides in Cancer Cells....Pages 95-107
Comparison of 5-Fluoro-2’-Deoxyuridine and 5-Fluorouracil in the Treatment of Murine Colon Cancer; Effects on Thymidylate Synthase....Pages 109-114
Intracellular Pharmacology and Biochemistry of Methotrexate and 6-Mercaptopurine in Childhood Acute Lymphoblastic Leukemia....Pages 115-118
Modulation of Arabinosylcytosine Metabolism during Leukemia Therapy....Pages 119-124
A New Mechanism of Toxicity of 2-Chlorodeoxyadenosine (2CdA)....Pages 125-128
The Influence of 2-Chlorodeoxyadenosine (2-CdA) Alone and in Combination with Cyclophosphamide or Methotrexate on Normal and Leukemic Hematopoiesis....Pages 129-130
Utilization of Purine and Pyrimidine in Human Gastric Cancer Cells (Kato III): Effect of a Nucleotide and Nucleosides Mixture (OG-VI) Solution on Proliferation with Coadministration of 5-Fluorouracil....Pages 131-134
Inhibition of the Proliferation of Human Cancer Cells In-Vitro by Substrate-Analogous Inhibitors of dUTPase....Pages 135-138
Synergistic Interaction between Cisplatin and Gemcitabine in Ovarian and Colon Cancer Cell Lines....Pages 139-143
Antimetabolites Reduce the Activities of Enzymes with Short Half-Lives in Addition to Inhibiting their Specific Targets....Pages 145-150
Inhibition of Murine Amido Phosphoribosyltransferase by Folate Derivatives....Pages 151-154
Inosine 5’-Monophosphate Dehydrogenase as a Chemotherapeutic Target....Pages 155-160
Pharmacokinetics/Pharmacodynamics of CI-1000, a Purine Nucleoside Phosphorylase (PNP) Inhibitor, in Rats and Monkeys....Pages 161-165
Blockade of Nucleoside Degradation in Monkey Whole Blood in Vitro by CI-1000, a Purine Nucleoside Phosphorylase (PNP) Inhibitor....Pages 167-171
Changes in Monkey Plasma Purines Induced by Repeated Doses of CI-1000, a Novel Inhibitor of Purine Nucleoside Phosphorylase....Pages 173-177
Inhibitors of Thymidylate Synthase and Glycinamide Ribonucleotide Transformylase....Pages 179-183
The Antitumour Activity of ZD9331, a Non-Polyglutamatable Quinazoline Thymidylate Synthase Inhibitor....Pages 185-188
Cross-Resistance to Thymidylate Synthase Inhibitors in P-Glycoprotein and Non-P-Glycoprotein Cell Lines....Pages 189-193
Nucleotide Metabolism: Mode of Action of Thiopurines in Leukemia....Pages 195-200
Mitochondrial Versus Cytosolic Activities of Deoxyribonucleoside Salvage Enzymes....Pages 201-204
Does Low-Dose Allopurinol, with Azathioprine, Cyclosporin and Prednisolone, Improve Renal Transplant Immunosuppression?....Pages 205-208
Purine Nucleotide Interconversion Enzymes in Childhood Leukemia: Relation with Cell Cycle and Clinical Outcome....Pages 209-212
Behavior of Enzymes Involved in Purine Nucleotide Metabolism in Tumors....Pages 213-218
Purine and Pyrimidine Metabolisms in Human Gliomas, Melanomas and Colon Carcinomas Xenografts, Relation to their Cytogenetic Profile....Pages 219-225
Ecto-5’-Nucleotidase Activity in Lymphocytes from Healthy and Leukemia Patients....Pages 227-230
Endo-5’-Nucleotidase Activity in Lymphocytes from Healthy Subjects and Leukemia Patients....Pages 231-235
Purine Nucleotide Content in the Leukocytes of Leukemia Patients....Pages 237-241
AMP-Deaminase from Human Uterine Muscle Neoplasm (Leiomyoma)....Pages 243-247
Regulation of Gene Expression of Adenosine Deaminase, Purine Nucleoside Phosphorylase and Terminal Deoxynucleotidyl Transferase by Dexamethasone and cAMP in Human Leukemic Cells....Pages 249-252
Altered Substrate and Inhibitor Specificity of Purified Human Adult Thymidine Kinases (TK2) from Leukemic Cells....Pages 253-256
Elevated Ratio between Deoxycytidine Kinase and Thymidine Kinase 2 in CLL Lymphocytes Compared to Control Cells....Pages 257-259
The Roles of Uridine-Cytidine Kinase and CTP Synthetase in the Synthesis of CTP in Malignant Human T-Lymphocytic Cells....Pages 261-264
Metabolism of UDP-N-Acetyl-Hexoses and UDP-Hexoses in Normal Human T-Lymphocytes and MOLT-3 T-Leukemia Cells....Pages 265-268
Pattern of Methylated Purine Bases in Urine of Cancer Patients. Analysis by Mass Spectrometry....Pages 269-273
Cytotoxicity of Deoxycoformycin on Human Colon Carcinoma Cell Lines....Pages 275-278
Imbalance between the Pyrimidine Ribonucleotide Pools in Rat Rhabdomyosarcoma R1 Cells....Pages 279-282
Compartmentation of Ribonucleotides in PC-12 Cells: Free and Protein Bound Ribonucleotides....Pages 283-286
The Effect of Ischemic Preconditioning on Nucleotide Metabolism and Function of Rat Heart after Prolonged Cold Storage....Pages 287-290
Does Adenosine Deaminase Inhibition Protect Ischemic Myocardium?....Pages 291-294
Is Hypoxanthine a Useful Marker of Perinatal Hypoxia?....Pages 295-298
Endothelial Cells’ Responses to Hypoxia and Reperfusion....Pages 299-302
Inhibition of Lipid Peroxidation by Purines and Analogues of Purine....Pages 303-306
A Study of Anoxia in Rat Hepatocytes....Pages 307-311
Adenine Nucleotide Levels and Lipid Peroxidation at Hypoxia and Reoxygenation in Different Cell Types....Pages 313-318
Purine and Pyrimidine Catabolite Production in the Postischemic Rat Heart — Effect of Adenosine Supply during Reperfusion....Pages 319-322
The AMP — Adenosine Cycle is Active During Normoxia and Impaired in ATP Depletion in Isolated Rabbit Cardiomyocytes....Pages 323-326
Search for the Mechanisms of High Incidence of APRT Deficiency Among Japanese....Pages 327-330
Genetic and Clinical Heterogeneity in Hypoxanthine Phosphoribosyltransferase Deficiencies....Pages 331-335
Molecular Mechanisms of the Second Female Lesch-Nyhan Patient....Pages 337-340
Clinical Symptoms of Patients with Partial HPRT Deficiency....Pages 341-344
Normal Hypoxanthine and Ammonia Release from Working Muscle in Partial HPRT Deficiency....Pages 345-348
Altered Pyridine Metabolism in the Erythrocytes of a Mentally Retarded Infant with Partial HPRT Deficiency....Pages 349-352
Treatment of Lesch-Nyhan Syndrome with AICAR....Pages 353-356
Plasma and Urinary Oxypurines in Lesch-Nyhan Patient after Allopurinol Treatment....Pages 357-361
Adenylosuccinate Lyase Deficiency: An Update....Pages 363-366
Adenylosuccinate Lyase Deficiency in a Czech Girl and Two Siblings....Pages 367-370
Anomalous Response to Intravenous Fructose Tolerance Test in a Case of Deficit of Adenylosuccinate Lyase....Pages 371-374
Effect of Allopurinol on the Xanthinuria in a Patient with Molybdenum Cofactor Deficiency....Pages 375-378
Differential Diagnosis of Main Rheumatic Diseases in Man....Pages 379-382
Dihydropyrimidinuria: The First Case in Japan....Pages 383-386
Normal Uric Acid Concentrations in a Purine Nucleoside Phosphorylase (PNP) Deficient Child Presenting with Severe Chicken Pox, Possible Immunodeficiency and Developmental Delay....Pages 387-389
Biochemical and Immunological Status Following Gene Therapy and PEG-ADA Therapy for Adenosine Deaminase (ADA) Deficiency....Pages 391-394
Genetic Manipulation of Hematopoietic Stem Cells in Adenosine Deaminase Deficiency....Pages 395-400
A1 Adenosine Receptors in Human Neutrophils: Electron Microscope Localization Using a Colloidal Cha-Gold-Albumin Probe....Pages 401-405
A 2 Adenosine Receptors in Neutrophils from Healthy Volunteers and Patients with Rheumatoid Arthritis....Pages 407-410
The Antiinflammatory Effects of Methotrexate are Mediated by Adenosine....Pages 411-416
Endogenous Adenosine Formation can Regulate Human Neutrophil Function....Pages 417-421
Acadesine: Preclinical Overview....Pages 423-426
Regulation of Endogenous Adenosine Levels in the CNS: Potential for Therapy in Stroke, Epilepsy and Pain....Pages 427-430
Enzymes of Adenosine Metabolism in the Heart, Cardiomyocytes and Endothelium....Pages 431-434
Adenosine Uptake and Metabolism in Human Endothelial Cells....Pages 435-438
Sah-Hydrolase Activity in Haemolysate and Intact Erythrocytes; The Effect of Adenosine Analogues....Pages 439-441
Chemotherapeutic Options in HIV Infection....Pages 443-451
Changes of Purine Nucleotide Content in Lymphocytes from Patients Infected with Human Immunodeficiency Virus....Pages 453-458
Anti-Retroviral and Pharmacological Properties of 9-(2-Phosphonylmethoxyethyl)Adenine (PMEA)....Pages 459-464
Properties of Mitochondrial DNA Metabolising Enzymes; Implications for Chemotherapy....Pages 465-469
Adenosine Deaminase (ADA) Deficiency as the Unexpected Cause of CD4+ T-Lymphocytopenia in Two HIV-Negative Adult Female Siblings....Pages 471-474
Serum Guanase Activity in Hepatitis C Virus Infection....Pages 475-482
Ganciclovir Nucleotide Analysis in Human Myocardial Tissue. Usefulness in the Diagnosis of Cytomegalovirus Myocarditis....Pages 483-485
Toxicity of Adenosine Analogues against Human Malaria ( Plasmodium falciparum )....Pages 487-491
Altered Purine Nucleoside Transport as a Target for Malaria Chemotherapy....Pages 493-498
Quantitative Determination of Trypanosoma cruzi Growth inside Host Cells in Vitro and Effect of Allopurinol....Pages 499-502
Polyamine Content of Acanthamoeba polyphaga at Different Stages of Development and the Effect of Pentamidine....Pages 503-508
Specific Enzyme Synthesizing Adenosine from Adenine and Ribose-1-Phosphate in Invertebrates....Pages 509-512
Molecular Characterization of a Carbamoyl-Phosphate Synthetase II (CPS II) Gene from Trypanosoma cruzi ....Pages 513-516
Development of a Mouse Model for the Study of Human Purine Metabolism....Pages 517-521
Effect of Galactosamine on Adenine and Uracil Nucleotide Levels in Isolated Hepatocytes of Young and Old Rats....Pages 523-526
Influence of Purines on Endothelial High Energy Phosphates....Pages 527-530
Ribose 1-P-Dependent ADP and ATP Formation in Rat Liver....Pages 531-535
Myocardial 5’Deoxy-5’Methylthioadenosine Phosphorylase....Pages 537-539
A Nucleoside Mixture and its Sparing Effect on de novo Purine Nucleotide Synthesis....Pages 541-544
 de novo Pyrimidine Nucleotide Biosynthesis in Synchronized Chinese Hamster Ovary Cells....Pages 545-548
The Catalytic Mechanism of Hamster Dihydroorotase....Pages 549-553
Stimulation by 6-Azauridine of de novo Pyrimidine Biosynthesis in BHK 165-23 Cells....Pages 555-558
Observed Resistance to Pyrimidine Analogs and Sensitivity to Uracil in Drosophila is attributed to Deregulation of Pyrimidine Metabolism....Pages 559-565
Expression of de novo Pyrimidine Biosynthesis Genes during Spermatogenesis in Drosophila melanogaster ....Pages 567-574
Regulation of Calf Thymus Cytosolic 5’-Nucleotidase/Nucleoside Phosphotransferase....Pages 575-578
Diverse Genetic Regulatory Elements are Required to Direct the Proper Tissue-Specific and Developmental Expression of the Murine Adenosine Deaminase Gene....Pages 579-584
The Muscle and Nonmuscle Isozymes of Adenylosuccinate Synthetase are Encoded by Separate Genes with Differential Patterns of Expression....Pages 585-590
Expressing Enzymatic Domains of Hamster CAD in CAD-Deficient Chinese Hamster Ovary Cells....Pages 591-595
Homology and Mutagenesis Studies of Hamster Dihydroorotase....Pages 597-601
Evolution of the GATase, CPSase, DHOase-LIKE, ATCase Multifunctional Protein in Eukaryotes: Genetic and Molecular Approaches with Yeasts S. cerevisiae and S. pombe ....Pages 603-606
Nucleoside Diphosphate Kinase: an Old Enzyme with New Functions?....Pages 607-611
Production of Adenosine and Nucleoside Analogues by an Exchange Reaction Catalyzed by Adenosine Kinase....Pages 613-616
Different Substrate Specificity of Two Isozymes of Cytosolic 5’-Nucleotidase from Rabbit Heart....Pages 617-621
Human Placental Cytosolic Purine 5’-Nucleotidase is Effectively Reactive with an Anti-Chicken Liver Enzyme Antibody....Pages 623-626
Characterization of the Adenine Phosphoribosyltransferase from Saccharomyces cerevisiae ....Pages 627-630
Differential Substrate Properties of Mammalian Ribonucleotide Reductase....Pages 631-635
Altered Kinetic Properties of Recombinant Human Cytosolic Thymidine Kinase (TK1) as Compared with the Native Form....Pages 637-640
The Promoter of the Cho APRT Gene Contains Three Regulatory Regions....Pages 641-645
The Mouse APRT Gene as a Model for Studying Epigenetic Gene Inactivation....Pages 647-652
Analysis of in Vivo Somatic Mutations at the APRT Locus....Pages 653-656
Identification of Polymorphic Markers Flanking the Human APRT Gene....Pages 657-660
Germline and Somatic Mutation at the APRT Locus of Mice and Man....Pages 661-664
Isolation and Characterization of Mutations in the Mouse APRT Gene that Encode Functional Enzymes with Resistance to Toxic Adenine Analogs....Pages 665-670
Analysis of APRT Mutations by Reverse-Transcription PCR....Pages 671-674
Molecular Characterization of a Novel Mutation in APRT Heterozygotes....Pages 675-678
Direct Evidence for a Hot Spot of Germline Mutation at HPRT Locus....Pages 679-682
Regulation of Low Km (Ecto) 5’-Nucleotidase Gene Expression in Leukemic Cells....Pages 683-687
Ecto-5’-Nucleotidase (CD73): Genomic Cloning and Characterization of Regions Upstream of the Translation Start Site....Pages 689-692
Molecular Cloning and Sequence Analyses of Rat Liver Dihydroorotate Dehydrogenase....Pages 693-697
Mutagenesis Studies of Conserved Residues in Mammalian Dihydroorotase....Pages 699-702
Gene Mutations for Human Erythrocyte AMP Deaminase Deficiency....Pages 703-706
Point Mutations in PRPS1 , the Gene Encoding the PRPP Synthetase (PRS) 1 Isoform, Underlie X-Linked PRS Superactivity Associated with Purine Nucleotide Inhibitor-Resistance....Pages 707-710
2’, 3’-Dideoxycytidine Phosphorylation by Recombinant Mouse and Human Deoxycytidine Kinase....Pages 711-714
Feedback of S. cerevisiae CPSase-ATcase: Selection, Cloning and Sequencing of Mutant Alleles....Pages 715-720
Gene Structure and Regulation of the Expression of the R1 and R2 Subunits of Mouse Ribonucleotide Reductase....Pages 721-724
Molecular Characterization of IMP Dehydrogenase in Acquired Resistance to Mycophenolic Acid....Pages 725-728
Function of the Polypeptide Chain Segment Connecting the Dihydroorotase and Aspartate Transcarbamylase Domains in the Mammalian Multifunctional CAD....Pages 729-733
Mapping a Gene that Determines Erythrocytic Guanosine-5’-Triphosphate Concentration (Gtpc) on Mouse Chromosome 9....Pages 735-738
Regulation of Mammalian Serine Hydroxymethyltransferase....Pages 739-739
Regulation and Role of Inosine-5’-Monophosphate Dehydrogenase in Cell Replication, Malignant Transformation, and Differentiation....Pages 741-746
Reciprocal Alterations of Enzymic Phenotype of Purine and Pyrimidine Metabolism in Induced Differentiation of Leukemia Cells....Pages 747-751
Extracellular Concentrations of Oxypurines in Xanthine Oxidase-Deficient Hepatoma-Derived Cell Line HuH-7....Pages 753-756
Induction of Cell Differentiation by IMPDH Antisense Oligomer in HL-60 and K562 Human Leukemia Cell Lines....Pages 757-760
Inhibition of CTP Synthetase Induces Differentiation of HL-60 Cells and Down-Regulation of the C-MYC Oncogene....Pages 761-764
Adenosine Transport and Metabolism in Cardiomyocytes of Hyperthyroid Rat....Pages 765-768
Nucleoside Transport and Metabolism in Lymphocytes, Polymorphonuclear Cells and Cerebral Synaptosomes....Pages 769-774
Inhibition of Nucleoside Transport by Reactive Oxygen Species in Bovine Heart Microvascular Endothelial Cells....Pages 775-778
Novel Nucleoside Transport Inhibitors of Natural Origin....Pages 779-782
Modification of Tumor Necrosis Factor (TNF) Production and Survival Rate by a Nucleoside Mixture in Lipopolysaccharide-Injected Rats....Pages 783-786
The Effect of Dietary Nucleic Acid Deficiency and the Administration of a Nucleotide and Nucleosides Mixture Solution on Endotoxin Shock in Rats....Pages 787-790
Radioimmunoassay of Folic Acid and its Correlation with Age....Pages 791-793
Uric Acid and Allantoin in Rat Liver after Oxonic Acid and 14 C-Formate....Pages 795-799
The Measurement of Deoxynucleotide (dNTP) Pools by Radioimmunoassay (RIA)....Pages 801-804
Rapid Simultaneous Measurement of Nucleotides, Nucleosides and Bases in Tissues by Capillary Electrophoresis....Pages 805-810
Application of Capillary Zone Electrophoresis for Rapid Screening of Enzymatic Defects in Purine Metabolism....Pages 811-814
A Reverse-Phase HPLC Method for Cyclic Nucleotide Phosphodiesterases Activity and Classification....Pages 815-820
Determination of the Activity of Recombinant Human Phosphoribosylpyrophosphate Synthetase Isoform 1 by a Non-Isotopic, One-Step Method....Pages 821-824
Back Matter....Pages 825-836