Protoplasts 1983: Poster Proceedings

Front Matter....Pages I-XIX
Front Matter....Pages 1-1
Protoplast Isolation from Morphogenetic Cultures of Rice Oryza Sativa L....Pages 2-3
Preliminary Studies on Isolation and Culture of Protoplasts from Sandalwood (Santalum Album)....Pages 4-5
Isolation and Identification of Enzymes that are Responsible for the Isolation of Plant Protoplasts....Pages 6-7
Isolation and Culture of Leaf Protoplasts from Ulmus sp.: Preliminary Report....Pages 8-9
Isolation of High Yields of Viable Protoplasts from Quaking Aspen Seedlings and Cultured Loblolly Pine Cell Suspensions....Pages 10-11
Approaches to Cereal Protoplast Culture: Morphogeny Cultures in Wheat (Triticum)....Pages 12-13
Towards a Protoplast Culture System for Wheat ( Triticum aestivum )....Pages 14-15
Approaches to Cereal Protoplast Culture: Analysis of Ustilago Maydis Induced Cell Divisions in Zea Mays....Pages 16-17
Mix-Culture of Nicotiana and Capsicum Mesophyll Protoplasts....Pages 20-21
Study of Hypocotyl and Mesophyll Protoplasts....Pages 22-23
Isolation and Culture of Cotyledon Protoplasts of Cotton ( Gossypium Barbadense cv. Giza 70)....Pages 24-25
Studies on Isolation of Protoplast from Some Oil-Yielding Plants: Niger ( Guizotia Abyssinica Cass) and Safflower ( Carthamus Tinctorius L.)....Pages 26-27
Developmental Potential of Mega and Normal Protoplasts in Populus....Pages 28-29
A Study of Protoplast Regeneration of Ornamental Species within the Compositae ....Pages 30-31
Callus Formation from Cotyledon Protoplasts of Browallia , Hyoscyamus and Physalis Species....Pages 32-33
Culture of Apical Protoplasts from Shoot Cultures in the Orders Fabales, Rosales and Caryophyllales....Pages 34-35
Production, Culture and Callus Formation from Leaf Protoplasts of Hedysarum Coronarium L. ....Pages 36-37
Sustained Division of Sugarbeet (Beta vulgaris) Protoplasts : Stimulating Effect of Conditioned Media....Pages 38-39
Plant Regeneration from Suspension Culture and Mesophyl Protoplaste of Alfalfa....Pages 40-41
Protoplast Isolation, Culture and Plant Formation of Psycomitrella....Pages 42-43
Front Matter....Pages 1-1
Optimisation of Turnip (Brassica Rapa) Protoplast Culture for Cauliflower Mosaic Virus Transformation....Pages 44-45
Lycopersicon Esculentum: Globular Embryos from Microspores and Calli from Diploid Protoplasts....Pages 46-47
Isolated Tetrad Protoplasts Develop to the Binucleate Stage in Tobacco (Nicotiana Tabacum CV Havana)....Pages 48-49
Protoplast Regeneration of Some Legume Species....Pages 50-51
Isolation and Culture of Coffee Leaf Protoplasts....Pages 52-53
Viability And Development Of Potato Protoplast Culture....Pages 56-57
Somatic Embryos and Plants from Cultured Protoplasts of Pennisetum Purpureum Schum. (Napier Grass)....Pages 58-59
Plant Regeneration from Mesophyll Protoplasts of White Clover ( Trifolium repens L.)....Pages 60-61
The isolation, purification and culture of Brassica Napus Cv. Lingot cotyledon protoplasts....Pages 62-63
Improved Culture Ability of the Genus Brassica by Using Hypocotyls as the Source for Protoplasts....Pages 64-65
Somatic Embryogenesis and Plant Regeneration from Protoplasts of Eggplant ( Solanum Melongena L.)....Pages 66-67
Plants Regenerated from Isolated Protoplasts of Solanum Brevidens....Pages 68-69
Factors Influencing the Frequency of Heterokaryon Formation in Forage Legume Protoplasts....Pages 72-73
Intergeneric Fusion of Therrestrial Orchid Protoplasts Induced by Different Fusion Promoting Agents....Pages 74-75
Complementation of Nitrate Reductase Deficient Mutants in Somatic Hybrids between Nicotiana Species....Pages 76-77
Towards Somatic Hybridization in the Genus Linum (Flax)....Pages 78-79
Assessment of Somatic Hybridisation between Lycopersicon Esculentum and L. Peruvianum ....Pages 80-81
Factors Affecting Somatic Hybridization between Sexually Incompatible Species of Petunia ....Pages 82-83
Ultrastructure and Isozyme Analysis of Cultured Soybean — Nicotiana Fusion Products....Pages 84-85
The Transfer of LpDH Activity as Marker in Somatic Hybrid Plant between Tobacco Tumour B6S3 and Normal Tobacco Xanthi....Pages 86-87
Front Matter....Pages 1-1
Production and Characterization of Albino Mutants for Use in Protoplast Culture....Pages 88-90
Increasing the Variability of Lycopersicon Mill. by Protoplast Fusion with Petunia L.....Pages 90-91
Carrot Ⓧ Tobacco Somatic Cell Hybrids Selected by Amino Acid Analog Resistance Complementation....Pages 92-93
Molybdenum-Cofactor Gene Transfer into Nitrate Reductase Deficient Plant Cells through Intergeneric Protoplast Fusion....Pages 94-95
Developmentally Regulated Fusion of Carrot Protoplasts....Pages 96-97
Genetic Behavior of Somatic Hybrids in the Genus Nicotiana : N. Otophora + N. Tabacum and N. Sylvestris + N. Tabacum ....Pages 98-99
Nitrate-Reductase Deficients in the Progeny After Selfing Complemented Somatic Hybrids of Nicotiana Plumbaginifolia Mutants....Pages 100-101
Fusion of Protoplasts Isolated from Chlorella Saccharophila (Krüger) Nadson 211-1a....Pages 102-103
Production of Somatic Hybrids Using Auxotrophic Nurse Cultures....Pages 104-105
Confirmation of Chloroplast Segregation in Somatic Hybrids of Datura by DNA-DNA Hybridization....Pages 106-107
Species Specific DNA Used to Identify Interspecific Somatic Hybrids....Pages 108-109
Hyoscyamus-Nicotiana Fusion Hybrids Selected via Auxotroph Complementation and Verified by Species-Specific DNA Hybridisation....Pages 110-111
Fusion Complementation Tests on a Group of Independently-Isolated Auxotrophic and Temperature-Sensitive Clones of Hyoscyamus Muticus and Nicotiana Tabacum....Pages 112-113
A Simplified Method for Bulk Production of Cytoplasts from Suspension-Culture Derived Protoplasts of Solanum Nigrum (L)....Pages 116-117
Identification of Plastomes and Chondriomes of Somatic Hybrid Plants Resulting from Protoplast Fusion by Molecular Probing of Fractionated DNA....Pages 118-119
Isolation, Purification and Sorting by Flow Cytometry of Metaphase Chromosomes of Haplopappus Gracilis ....Pages 120-121
First Steps to Chromosome Transplantation with Haplopappus Gracilis Using Flow Cytometry....Pages 122-123
Selection for Cytoplasmic Streptomycin Resistance after Protoplast Fusion as a Tool for Transfer of Cytoplasmic Male Sterility /CMS/ in Nicotiana ....Pages 124-125
Rescue of the Cytoplasmic Lincomycin Resistance Factor from Nicotiana Sylvestris into Nicotiana Plumbaginifolia by Protoplast Fusion....Pages 126-127
Chimeric Genes as Dominant Selectable Markers in Plant Cells....Pages 130-131
Front Matter....Pages 1-1
Isolation of Tobacco Clones Resistant to Naphtalene Acetic Acid and Affected in Root Morphogenesis....Pages 156-157
Isolation of Amino Acid — Requiring Lines by Negative Selection in Haploid Protoplasts of N. Plumbaginifolia....Pages 158-159
Attempt to Isolate Auxotrophic Mutants, using Protoplasts of Nicotiana Sylvestris ....Pages 160-161
Selection and Characterization of Nitrate Reductase Deficient Mutants of Petunia ....Pages 162-163
The Advantages of Isolated Protoplasts for Plant Growth Regulator Studies....Pages 178-179
Evidence for Extraosmotic Absorption of Water by Radish Tubers....Pages 180-181
Comparison of Tobacco Mosaic Virus (TMV) Synthesis in Protoplasts from Soybean Cells in Suspension Culture and Protoplasts from Tobacco Leaves....Pages 166-167
Use of Potato Protoplasts in Regeneration and Virus Resistance Studies....Pages 168-169
Lectin — Like Material Responsible for Specific Attachment of Pseudomonas Syringae PV. Glycinea to Resistant Soybean Leaf Cells....Pages 172-173
Protoplast Yields are Low and Tissue Browning is High in Potato Leaves Resistant to the Late Blight Fungus Phytophthora Infestans....Pages 174-175
Colony Formation during Joint Cultivation of Tobacco Protoplasts and Bacterial Plasmids....Pages 132-133
Protoplast and Chloroplast of Chlorella , the ARS Gene of Chlorella Chloroplast Replicates in Yeast....Pages 134-135
Expression of Foreign Genes in Plant Cells Following Fusion of Agrobacterium Spheroplasts with Isolated Mesophyll protoplasts of Nicotiana tabacum ....Pages 136-137
Proliferation of Cauliflower Mosaic Virus in Protoplast-Derived Clones of Turnip (Brassica Rapa)....Pages 138-139
A Reproducible Method for the Introduction of Nucleic Acids in Tobacco Protoplasts....Pages 140-141
Protoplast Culture Induces Genetic Variability in Nicotiana Sylvestris ....Pages 144-145
Variability of Different Cell Clones Issued from one Catharanthus Roseus Tissue Strain by Protoplast Isolation....Pages 146-147
Protoclonal Variation in a Dutch Commercial Cultivar of Potato ( Solanum Tuberosum L. cv. Bintje)....Pages 148-149
Variation in Plants Regenerated from Protoplasts and Complex Explants of Potato....Pages 150-151
Somaclonal Variation in Protoplast Derived Plants of Su/su Heterozygotes of Nicotiana Tabacum ....Pages 152-153
Front Matter....Pages 1-1
Microfluorometric Determination of Early Regeneration of Moss Protoplasts of Ceraton purpureus (Hedw.)....Pages 182-183
Oleic Acid as a Bioche — Mical Marker to Study Growth Photoregulation of Internode Protoplasts....Pages 184-185
Detection of Different Forms of O-Acetyl-L-Serine Sulfhydrylase in Mesophyll Protoplasts and Bundle Sheath Cells from Wheat and Maize Leaves....Pages 186-187
Isolation of Anthocyanin-Containing Vacuoles From Protoplast of a Cell Suspension Culture of Daucus Carota....Pages 188-189
Formation of Various Fractions of Structural Polysaccharides in the Course of Cell Wall Regeneration by Tobacco and Bean Protoplasts....Pages 190-191
The Role of Photosynthesis in the Process of Cell Wall Regeneration by Isolated Protoplasts....Pages 192-193
Ultrastructural Dynamics of Cell Wall Regeneration Around Isolated Bean Leaves Protoplasts....Pages 194-195
Non-Specific Inhibition of Cell Division in Regenerating Tobacco Protoplasts by Inhibitors of Ethylene Biosynthesis....Pages 196-197
Isolation of Vacuoles via Protoplasts from Sugarcane Stalk Tissue....Pages 198-199
Evolution of the Protoplast Budding Rate Versus the Plant Physiological State....Pages 200-201
Use of Maize Root Protoplasts for Herbicide Absorption Studies....Pages 202-203
Distribution of Vacuolar pH Values in a Population of Acer Pseudoplatanus Protoplasts....Pages 204-205
Modulation of Fatty Acid Patterns During Protoplast Isolation....Pages 206-207
Protoplast Viability — A Relative Concept....Pages 208-209
Reorganization of Microtubules in Protoplasts of Vicia Hajastana , Grossh. During the First 48 Hours of Culturing....Pages 212-213
Brief Cellulase Treatment Permits Anti-Tubulin Staining of an Entire Filamentous Organism (Moss)....Pages 214-215
Coated Vesicles From Plant Protoplasts....Pages 216-217
Mesophyll Protoplasts and Plant Regeneration of Brassica Napus : Cytology and Morphology of Callus Growth....Pages 218-219
Selection of Monohaploid Potato Genotypes to Produce Stable Shootcultures as a Source of Haploid Protoplasts for Work on Recessive Mutations....Pages 220-221
Ultrastructure of Intermediate Filament Bundles Associated with the Cytoskeleton of Protoplasts and Cells from Maize ( Zea mays L.) Suspensions....Pages 222-223
Front Matter....Pages 1-1
Gene Expression in Freshly Isolated Protoplasts from Nicotiana Sylvestris....Pages 240-241
Hormonal Control OF Protein Synthesis in Tobacco Protoplasts....Pages 242-243
Immobilisation of Nicotiana Cells and Protoplasts in Alginate Beads....Pages 226-227
Flow Cytometry and Sorting on pH and Alkaloids in Catharanthus Protoplasts....Pages 228-229
Obtention of Protoplasts of Sylibum Marianum and its Use in the Coagulation of Milk....Pages 230-231
A Screening for Shoot Regeneration in Root Cultures of Solanaceae Species....Pages 246-247
Somatic Hybridization and Cryopreservation Studies on Rice x Pea and Wheat x PEA....Pages 248-249
Tissue Culture Studies on Safflower ( Carthamus Tinctorius L.)....Pages 250-251
Preliminary Investigations on in vitro Fertilization in Cowpea V. Unguiculata (L.) Walp....Pages 252-253
Detection of Tobacco Mosaic Virus in Tissues and Protoplasts by dot Molecular Hybridization....Pages 256-257
Improved culture ability of Shoot Culture Derived Protoplasts of Solanum Tuberosum L. by Use of activated charcoal....Pages 258-259
Immobilization of Daucus Carota Protoplasts....Pages 260-261
Characterisation and Separation of Petunia Hybrida and Lycopersicon Esculentum Protoplasts Based on Nutural Fluorescence or FITC Added to Labell Antibodies in Immunoreaction with the Protoplast Membrane....Pages 262-263
Size Fractionation of plant Protoplasts by Centrifugal Elutriation....Pages 264-265
Culture in Agarose Improves Protoplast Plating and Proliferation, and Permits Division in Otherwise Unresponsive Systems....Pages 266-267
Attempts to Develop a Plant Analogue to the Oocyte System....Pages 268-269
Microcarrier-Anchored Plant Protoplasts....Pages 270-271
Production of Monoclonal Antibodies Directed Against Developmentally-Regulated Protoplast Antigens....Pages 234-235
Heritable Differences in in vitro Regenerability in Petunia,at the Protoplast and at the Seedling Stage....Pages 236-237
Steps Towards the Introduction of Bremia Resistance in Lactuca Sativa Using Tissue Culture Methods....Pages 274-275
Front Matter....Pages 1-1
Intra- and Intersubfamilial Somatic Hybridisation within the Solanaceae....Pages 276-277
Somatic Hybrization of Medicago Sativa and Medicago Falcata ....Pages 278-279
Selection of Somatic Hybrids by Fusion of Protoplasts from Brassica Oleracea and B. Campestris....Pages 280-281
Plant Regeneration from Protoplasts of Different Potato Genotypes....Pages 282-283
Protoplast Isolation, Plant Regeneration and Somatic Hybridization in Different Citrus Species and Microcitrus ....Pages 284-285
Intergeneric Cytoplasm Hybridization in Cruciferae by Protoplast Fusion....Pages 286-287
Front Matter....Pages 289-289
Electro — Fusion of Yeast Protoplasts....Pages 290-291
The Preparation of Protoplasts from an Oleaginous Yeast....Pages 292-293
Efficient Protoplast Isolation from Coprinus Macrorhizus and Other Basidiomycetes....Pages 294-295
Mating Pheromone Enhances Hybrid Formation: Protoplast Fusion in the Yeast Saccharomyces cerevisiae ....Pages 296-297
Fusion of Protoplasts from Conidiospores of Aspergillus Nidulans....Pages 298-299
Protoplasts from Mutants of Aspergillus Niger Using the Dialysis Membrane Technique....Pages 300-301
Preparation and Regeneration of Escherichia Coli Protoplasts....Pages 302-303
An Improved Method for the Production of Protoplasts of Podospora anserina ....Pages 304-305
Spontaneous Recombination of Mitochondrial Genomes in Interspecific Somatic Hybrids of Aspergillus....Pages 308-309
Hybridization of Penicillium Chrysogenum and Penicillium Baarnense by Protoplast Fusion; Genetic and Biochemical Analysis....Pages 310-311
Protoplast Fusion and Genetic Analysis in Cephalosporium Acremonium ....Pages 312-313
Genetic Effects of Protoplast Fusion in Penicillium Chrysogenum ....Pages 314-315
Intervariant and Interspecific Recombinants of Bacillus Thuringiensis Obtained by Protoplast Fusion....Pages 316-317
Protoplast Fusion to Overcome Vegetative Incompatibility in Verticillium Lecanii Parasexual Genetics....Pages 318-319
Front Matter....Pages 289-289
Detection and Location of Vegetative Incompatibility Genes Operating between Species of Aspergillus....Pages 320-321
Protoplast Fusion within Aspergillus Nidulans Group by Nutritional Selection....Pages 322-323
Mutagenesis of Micromonospora Rosaria Using Protoplasts and Mycelial Fragments....Pages 324-325
Complementation in Fusions between E. Coli and Bacillus Subtilis ....Pages 326-327
A Search for Biparentals in Bacillus Megaterium ....Pages 328-329
In Vitro, In Vivo Candida, Albicans Spheroplast (Protoplast) Studies....Pages 332-333
Respiration of Protoplasts Isolated from Aspergillus Nidulans ....Pages 334-335
Visualization of Gold Marker at the Surface of Protoplasts and Cell Wall Remnants in Yeast Saccharomyces by Extraction Replica Method....Pages 336-337
Fusion of cdc Mutants: Possible Effect of Cell Cycle on the Fusion of Yeast Protoplasts....Pages 338-339
Penicillin Production by Aspergillus Hybrids Obtained by Protoplast Fusion....Pages 342-343
The use of the Protoplast Fusion Method in Selection of Streptomyces griseus, the Producer of Streptothricine Antibiotic Grisin....Pages 344-345
Preparation of Fungal Protoplasts for Steroid Transformation....Pages 346-347
Protoplast Fusion in Strain Improvement of Micromonospora Inyoensis , a Sisomicin-Producer....Pages 348-349
Genetic Reassortment after Protoplast Fusion in the Fungus Gibberella Fujikuroi ....Pages 350-351
Cloning and Expression of Bacillus Amyloliquefaciens α -Amylase in Yeast Saccharomyces Cerevisiae....Pages 354-355
Towards Transformation of the Yeast Saccharomyces cerevisiae with an E. coli Gene for D-Xylose Isomerase....Pages 356-357
Some Genetic Applications of Streptomyces Protoplasts....Pages 358-359
Protoplast Fusion and Protoplast Transformation in Micromonospora Species....Pages 360-361
Back Matter....Pages 362-366