Proteins: Structure and Function

Front Matter....Pages iii-xv
Front Matter....Pages 1-1
Use of Short Microbore HPLC Columns for the Concentration, Separation and Recovery of Subnanomole Amounts of Protein and Polypeptides for Microsequence Analysis....Pages 5-20
Practical and Theoretical Aspects of Microbore HPLC of Proteins, Peptides and Amino Acid Derivatives....Pages 21-28
Microisolation and Sequence Analysis of Human Epidermal Growth Factor....Pages 29-36
Complete Amino Acid Sequence Determination of Rat Epidermal Growth Factor:Characterization of a Truncated Form with Full In Vitro Biological Activity....Pages 37-44
Use of HPLC Comparative Peptide Mapping in Structure/Function Studies....Pages 45-52
Precolumn Derivatization of Peptides with Phenylisothiocyanate (PITC)....Pages 53-59
High-Performance Liquid Chromatography as a Means of Characterizing Isoforms of Steroid Hormone Receptor Proteins....Pages 61-74
Large Scale Purification of a Human Hepatoma Derived Endothelial Cell Growth Factor....Pages 75-81
Very Rapid Microanalysis of IgG in Ascites Fluids by HPLC Using a Novel Anion-Exchange Column....Pages 83-90
Application of Immunoaffinity Chromatography to the Purification of Polypeptides for Microsequence Analysis....Pages 91-101
A Novel Approach to Isolation of Proteins for Microsequence Analysis: Electroblotting Onto Activated Glass....Pages 105-119
Blotting of Protein-Detergent Complexes on Glass and Glass Coated with Polybases: A Comparative Study. Acid Hydrolysis and Gas-Phase Sequencing of Gel-Separated Proteins Immobilized on Polybrene Glass-Fiber....Pages 121-131
Two-Dimensional Gel Electrophoresis of Polypeptides....Pages 133-138
Carboxymethylated Hemoglobin as a Structural Analogue for Carbamino Hemoglobin....Pages 141-147
Gossypol: Interaction with Ribonuclease A....Pages 149-154
Studies on Nonenzymic Glycosylation of Peptides in a Simple Model System....Pages 155-160
Reaction of Glyceraldehyde (Aldotriose) with Proteins is a Prototype of Nonenzymic Glycation: Protein Cross-Linking as a Consequence of in Vitro Nonenzymic Glycation....Pages 161-168
Reversible Dihydroxypropylation of Amino Groups in Proteins: Application in Primary Structural Studies of Streptococcal M-Proteins....Pages 169-176
Enzymatic Carboxylmethylation and Non-Enzymatic Demethylation at L-Isoaspartyl Residues: Possible Applications in Peptide Chemistry....Pages 177-184
A Practical Guide to the General Application of PTC-Amino Acid Analysis....Pages 187-195
Front Matter....Pages 1-1
High Performance Liquid Chromatography of Phenylthiohydantoin and Phenylthiocarbamyl Amino Acids....Pages 197-205
Amino Acid Analysis Using Pre-Column Derivatization with Phenylisothiocyanate: Matrix Effects and Tryptophan Analysis....Pages 207-213
An Improved Reverse Phase HPLC Separation and Postcolumn Detection of Amino Acids....Pages 215-223
HPLC Assay of Phosphoamino Acids by Fluorescence Detection....Pages 225-231
Amino Acid Analysis by Reverse-Phase High Performance Liquid Chromatography: Separation of Phenylthiocarbamyl Amino Acids by Spherisorb Octadecylsilane Columns....Pages 233-238
Fast Atom Bombardment Mass Spectrometry: Application to Peptide Structural Analysis....Pages 241-249
Protein Sequence Determination and FAB Mass Spectrometry....Pages 251-268
The Use of HPLC and Mass Spectrometric Techniques to Quantify Endogenous Opioid Peptides....Pages 269-282
Structural Analysis of Proteins of the Nervous System....Pages 283-298
Pancreatic Preprosomatostatin Processing: Isolation and Structure of Intermediates and Final Products of Processing....Pages 299-304
Progress Toward Polybrene Purification and Utility in Micro-Peptide/Protein Sequencing....Pages 307-313
Approaches for Microsequencing: Extraction from SDS Gel, Composition, Terminal Analysis....Pages 315-322
Manual Gas Phase Microsequencing on Filterchips for Simultaneous Edman Degradation of Multiple Peptide Samples....Pages 323-332
Strategies for Sequencing Peptide Mixtures by Selective Blocking....Pages 333-341
Amidation of Protein Carboxyl Groups....Pages 343-353
A Method for Direct Amino Acid Sequence Analysis of the NH 2 -Terminal Regions of Fibrinogen....Pages 355-360
PTH Amino Acid Analysis....Pages 363-381
An On-Line Isocratic HPLC System for the Analysis of PTH-Amino Acids on A Gas-Phase Sequencer....Pages 383-394
Routine Analysis of Low-Picomole-Level Phenylthiohydantoins by HPLC Using a Diisopropylethylamine-Acetate/THF Buffer and Acetonitrile Gradient....Pages 395-402
Identification of Side-Chain Protected L-Phenylthiohydantoins on Cyano HPLC Columns: An Application to Gas-Phase Microsequencing of Peptides Synthesized on Solid-Phase Supports....Pages 403-413
Front Matter....Pages 1-1
Separation of Aminoacid Phenylthiohydantoin Derivatives by High Pressure Liquid Chromatography....Pages 415-422
Hydrophobic Moments as Tools for Analysis of Protein Sequences and Structures....Pages 425-436
Identification of Protein Surfaces and Interaction Sites by Hydrophilicity Analysis....Pages 437-443
The Protein Identification Resource (PIR): An On-Line Computer System for the Characterization of Proteins Based on Comparisons with Previously Characterized Protein Sequences....Pages 445-453
Computer Analysis of Protein Sequencing Data....Pages 455-460
Simultaneous Multiple Peptide Synthesis: The Rapid Preparation of Large Numbers of Peptides....Pages 463-470
Mapping Functional Domains of Human Platelet Thrombospondin with Electro-Blotting and High Sensitivity Sequencing....Pages 471-477
Immunoprotection — A Novel Approach for Mapping Epitopes on an Antigen....Pages 479-486
Micro Method for the Detection of Heparin-Binding Proteins and Peptides....Pages 487-492
Rapid and Sensitive Determination of Protein Disulfide Bonds....Pages 493-501
Simulation of Protein Hydrodynamic Changes Observed by Urea Gradient Gel Electrophoresis....Pages 503-507
Front Matter....Pages 509-509
Site-Specific Mutations in Dihydrofolate Reductase at the Dihydrofolate Binding Site....Pages 513-519
Site Saturation Mutagenesis of Active Site Residues of β-Lactamase....Pages 521-528
Enzyme Thermostability and its Enhancement by Genetic Engineering....Pages 529-537
Expression of Human C5a in E. Coli....Pages 539-549
Use of Trinitrobenzene Sulfonate to Determine the p K a Values of Two Active-Site Lysines of Ribulosebisphosphate Carboxylase/Oxygenase 1 ....Pages 553-568
Advances in Affinity Labeling of Purine Nucleotide Sites in Dehydrogenases....Pages 569-580
 Escherichia Coli ADPglucose Synthetase Substrate-Inhibitor Binding Sites Studied by Site(S) Directed Chemical Modification and Mutant Enzyme Characterization....Pages 581-598
Active Site and Other Sequence Data from Torpedo Californica Acetylcholinesterase....Pages 599-605
Acetylcholine Receptor α-Bungarotoxin Interactions Studied by Chemical Modification, Enzyme Digestion and HPLC....Pages 607-614
Front Matter....Pages 509-509
Identification of Functional Domains of the Inhibitor Protein of Camp-Dependent Protein Kinase....Pages 631-639
Atrial Natriuretic Factor Receptor in Adrenal Plasma Membrane: Identification by Photo-Affinity Labeling....Pages 641-648
Structural Analysis of a 29/38kDa Heparin-Binding Domain of Fibronectin: Evidence that Two Different Subunits of Human Plasma Fibronectin Arise by Alternative mRNA Splicing....Pages 649-656
Structural and Functional Studies on the Purified Insulin Receptor....Pages 657-663
Localization and Interaction of Functional Sites on Antithrombin III. Use of an Anti-Hapten Antibody as a Structural Probe....Pages 665-672
Human C3b/C4b Receptor (Cr1): Isolation, Protein Sequence Analysis, and Cloning of a Partial cDNA from Human Tonsil....Pages 733-739
HTLV-III/LAV particle-associated proteins: I. Virus purification, inactivation and basic characterization....Pages 741-747
Structure and Function of Fish Antifreeze Polypeptides....Pages 749-756
Sequence of a Glycine-Rich Protein from Lizard Claw: Unusual Dilute Acid and Heptafluorobutyric Acid Cleavages....Pages 757-764
Primary Structure Studies on Serine Hydroxymethyltransferase....Pages 765-770
The Proteins in Avian Eggs: A Test of Protein Separation Methods Based On HPLC....Pages 771-777
Amino Terminal Sequence of 7B2: A Novel, Human Pituitary/Brain Polypeptide Present in Gonadotrophs and Released by LHRH....Pages 779-786
Imidoesters and the Mechanism of Liver Alcohol Dehydrogenase....Pages 615-622
Investigation of Gramicidin Channel Function by Single Amino Acid Replacement Using Non-Genetic Code Amino Acids....Pages 623-628
Properties of the Hydrolase that Catalyzes Removal of the Blocked NH 2 -Terminal Amino Acid Residues from Polypeptides....Pages 675-681
Kinetic Product Analysis of Aspartyl Proteinases Utilizing New Synthetic Substrates and Reversed Phase HPLC....Pages 683-688
The Esterase-Like Activity of Covalently Bound C3....Pages 689-696
Structural Analysis of Carcinoembryonic Antigen (CEA) and a Related Tumor-Associated Antigen (TEX)....Pages 699-710
Determination of the Location of N G , N G -Dimethylarginine in a Glycine-Rich Region of Nucleolar Protein C23....Pages 711-716
Structural Characterization of a Murine Lymphocyte Homing Receptor Suggests a Ubiquitinated Branched-Chain Glycoprotein....Pages 717-730
Front Matter....Pages 787-787
The Symposium Test Peptide (STP): Synthesis and Characterization of a Model Peptide....Pages 789-795
Workshop in Microanalytical Methods — Experiences with an Unknwon Synthetic Peptide....Pages 797-802
Back Matter....Pages 803-817