Proteinase Inhibitors: Proceedings of the 2nd International Research Conference

Front Matter....Pages I-XVI
Front Matter....Pages 1-1
A Turbulent Life....Pages 2-10
Welcome....Pages 11-12
Front Matter....Pages 13-13
Biochemistry of Proteinase Inhibitors from Human Plasma: A Review of Recent Development....Pages 14-22
Purification, Properties, and Composition of α 1 -Trypsin Inhibitor from Human Plasma....Pages 23-30
Properties of Human α 1 -Antitrypsin....Pages 31-39
Human α 1 -Antitrypsin Phenotypes....Pages 40-46
Isolation and Fragmentation of α 1 -Antitrypsin....Pages 47-52
The Interaction of Human Cationic Trypsin and Chymotrypsin II with Human Serum Inhibitors....Pages 53-62
Studies on the Quaternary Structure of Human Serum α 2 -Macroglobulin....Pages 63-71
The Unique Nature of the Interaction of α 2 -Macroglobulin with Proteinases....Pages 72-77
Biology and Pathology of Plasma Proteinase Inhibitors....Pages 78-95
Interaction between Endogenous Proteases and Plasma Protease Inhibitors in vitro and in vivo ....Pages 96-105
Plasma Clearance of Human Antiproteinase/Proteinase Complexes....Pages 106-108
Protease Inhibitors in Human Synovial Fluids of Patients with Joint Diseases....Pages 109-110
Proteinase Inhibitors of the Respiratory Tract: Studies on the Structural Relationship between Acid-Stable Inhibitors Present in the Respiratory Tract, Plasma and Urine....Pages 111-116
Front Matter....Pages 117-117
Biochemistry and Clinical Significance of the Trypsin-Like Proteinase Acrosin from Boar and Human Spermatozoa....Pages 118-127
Studies on the Purification and Characterization of Boar Acrosin....Pages 128-135
Proteinase Inhibitors and Proteinases of Human Semen....Pages 136-146
Characterization of Two Proteinase Inhibitors from Human Seminal Plasma and Spermatozoa....Pages 147-155
Isolation of Proteinase Inhibitors from Boar Sperm Acrosomes and Boar Seminal Plasma and Effect on Fertilization....Pages 156-163
Front Matter....Pages 117-117
Purification, Characterization, and Structural Studies of Proteinase Inhibitors from Boar Seminal Plasma and Boar Spermatozoa....Pages 164-177
Proteinase Inhibitors in Human Cervical Mucus and Their in vitro Interactions with Human Acrosin....Pages 178-186
Front Matter....Pages 187-187
Human Pancreatic Secretory Trypsin Inhibitor....Pages 188-198
Isolation of Human Pancreatic Inhibitor and the Study of Its Interaction with Mammalian and Human Proteases....Pages 199-212
Studies on Inhibition of the Two Human Trypsins....Pages 213-222
Sequence of Cleavages in Temporary Inhibitors: Porcine Inhibitor Form II and Bovine Isoinhibitors A and C....Pages 223-234
Temporary Inhibition— A Result of Consecutive Peptide Bond Hydrolysis....Pages 235-242
Measurement of the Bovine Pancreatic Trypsin Inhibitors by Radioimmunoassay....Pages 243-249
Properties and Inhibition Spectrum of a New Human Pancreatic Protease....Pages 250-251
Front Matter....Pages 253-253
Amino Acid Sequence of Snail Inhibitor K and Correlation of Structure and Specificity....Pages 254-264
Novel Proteinase Inhibitors in Snake Venoms: Distribution, Isolation, and Amino Acid Sequence....Pages 265-276
Broad Specificity Inhibitors from Sea Anemones....Pages 277-281
Discussion Remark....Pages 282-283
Discussion Remark....Pages 284-285
The Partial Linear Sequence of Garden Bean Inhibitor II and Location of the Protease Reactive Sites....Pages 286-290
Elastase Inhibitors as Impurities in Commercial Preparations of Soybean Trypsin Inhibitor (Kunitz)....Pages 291-304
A New Alkaline Proteinase Inhibitor from Streptomyces albogriseolus ....Pages 305-307
Front Matter....Pages 309-309
Replacements, Insertions, and Modifications of Amino Acid Residues in the Reactive Site of Soybean Trypsin Inhibitor (Kunitz)....Pages 311-324
Chemical Structure and Inhibitory Activities of Soybean Proteinase Inhibitors....Pages 325-343
Structure-Function Relationships in Lima Bean Protease Inhibitor....Pages 344-354
Front Matter....Pages 309-309
Structure-Activity Relationships of Several Trypsin and Chymotrypsin Inhibitors from Legume Seeds....Pages 355-361
Reactive Site Cleavage, Thermodynamic Control Resynthesis, and Properties of Chemically Derivatized Trypsin-Kallikrein-Inhibitors....Pages 362-377
The Interaction of Catalytically Inactive Proteolytic Enzymes with Protein Inhibitors Methyl-Chymotrypsin....Pages 378-383
Discussion Remark....Pages 384-385
Front Matter....Pages 387-387
Kinetics and Thermodynamics of the Interaction of Proteinases with Protein Inhibitors....Pages 389-411
Kinetic Studies of the Binding of Bovine Basic Pancreatic Trypsin Inhibitor to α-Chymotrypsin....Pages 412-419
The Mechanism of Association of Trypsin (or Chymotrypsin) with the Pancreatic Trypsin Inhibitors (Kunitz and Kazal) Kinetics and Thermodynamics of the Interaction....Pages 420-431
Kinetic Studies on the Interactions between Native, Acetylated and Succinylated Trypsin and Natural Proteinase Inhibitors....Pages 432-444
The Use of Fluorescence Techniques in the Study of the Interaction of the Basic Trypsin Inhibitor of Bovine Pancreas, Selectively Labelled at Lysine 15, with Chymotrypsin and Trypsin....Pages 445-453
Raman Studies of Native and Partially (Cys 14-Cys 38) Reduced Basic Pancreatic Trypsin Inhibitor....Pages 454-457
A Study on the Interaction of Trasylol with Trypsin and Kallikrein by Circular Dichroism....Pages 458-462
Some Kinetic Consequences of the Tight Binding of Protein-Proteinase-Inhibitors to Proteolytic Enzymes and their Application to the Determination of Dissociation Constants....Pages 463-469
Front Matter....Pages 471-471
Stereochemistry of Substrate Binding and Hydrolysis in the Trypsin Family of Enzymes....Pages 473-483
The Model of the Basic Pancreatic Trypsin Inhibitor Refined at 1.5 Å Resolution....Pages 484-496
Structure of the Complex Formed by Bovine Trypsin and Bovine Pancreatic Trypsin Inhibitor Refinement of the Crystal Structure Analysis....Pages 497-512
The Mode of Action of Soybean Trypsin Inhibitor as Revealed by Crystal Structure Analysis of the Complex with Porcine Trypsin....Pages 513-520
Front Matter....Pages 521-521
Organic Chemical Models for Proteinase Inhibitors....Pages 523-530
Progress in Designing Small Inhibitors which Discriminate among Trypsin-Like Enzymes....Pages 531-540
Homoarginine Oligomers as Trypsin and Chymotrypsin Substrates and Inhibitors....Pages 541-547
Proteinase Inhibitors of Microbial Origin A Review....Pages 548-559
Front Matter....Pages 521-521
Discussion Remark....Pages 560-561
Front Matter....Pages 563-563
Carboxypeptidase Inhibitor from Potatoes, Properties and Amino Acid Sequence....Pages 565-573
Inhibitors of Lysosomal Proteinases....Pages 574-580
Inhibition of Insulin Specific Protease by Proteinase Inhibitors from the Snail (Helix pomatia)....Pages 581-585
An Insulin Degrading Proteinase from Human Erythrocytes and Its Inhibition by Proteinase Inhibitors....Pages 586-593
Front Matter....Pages 595-595
Evolution of Specificity of Protein Proteinase Inhibitors....Pages 597-611
Front Matter....Pages 613-613
Proteases and Protease Inhibitors in Neoplasia....Pages 615-620
Secretion of Enzymes by Neoplastic Cells and Macrophages....Pages 621-630
Plasminogen Activators of Normal and Transformed Cells....Pages 631-648
Proteinase Inhibitors from Ascaris lumbricoides : Properties and their Physiological Role....Pages 649-666
Wound-Induced Accumulation of Proteinase Inhibitor I in Tomato Leaves....Pages 667-673
Front Matter....Pages 675-675
General Discussion Remark: Energetics of Inhibitor Binding....Pages 677-678
Resumé....Pages 679-684
Concluding Remarks....Pages 685-685
Back Matter....Pages 687-754