Protein Structure and Engineering

Front Matter....Pages i-ix
Introduction....Pages 1-2
Exploitation of Geometric Redundancies as a Source of Phase Information in X-Ray Structure Analysis of Symmetric Protein Assemblies — Including a worked example: the three-dimensional structure of the icosahedral β 60 capsid of heavy riboflavin synthase from Bacillus subtilis ....Pages 3-33
 Escherichia Coli Aspartate Transcarbamylase: The Relationship Between Structure and Function....Pages 35-48
Fundamentals of Neutron Diffraction....Pages 49-59
Purification of Complexes between Peptide Antigens and Class II Major Histocompatibility Complex Antigens Using Biotinylated Peptides....Pages 61-68
NMR Method for Protein Structure Determination in Solution....Pages 69-78
Determination of Structural Uncertainty from NMR and Other Data: The Lac Repressor Headpiece....Pages 79-95
The Generation of Three-Dimensional Structures from NMR-Derived Constraints....Pages 97-109
2D-NMR for 3D-Structure of Membrane Spanning Polypeptides: Gramacidin A and Fragments of Bacteriorhodopsin....Pages 111-138
The Dynamics of Oligonucleotides and Peptides Determined by Proton NMR....Pages 139-154
Methods of Stable-Isotope-Assisted Protein NMR Spectroscopy in Solution....Pages 155-192
NMR Studies of Protein Dynamics and Folding....Pages 193-207
Understanding the Specificity of the Dihydrofolate Reductase Binding Site....Pages 209-220
Histone H1 Solution Structure and the Sealing of Mammalian Nucleosome....Pages 221-241
 1 H NMR Studies of Genetic Variants and Point Mutants of Myoglobin: Modulation of Distal Steric Tilt of Bound Cyanide Ligand....Pages 243-256
Spectroscopy of Molecular Structure and Dynamics....Pages 257-267
Molecular Dynamics: Applications to Proteins....Pages 269-290
Protein Engineering and Biophysical Studies of Metal Binding Proteins....Pages 291-308
Growth Hormones: Expression, Structure and Protein Engineering....Pages 309-324
Cloning, Sequencing and Expression of a New β-Galactosidase from the Extreme Thermophilic Sulfolobus Solfataricus ....Pages 325-331
Site-Directed Mutagenesis and the Mechanism of Flavoprotein Disulphide Oxidoreductases....Pages 333-346
Resonance Raman and Site-Directed Mutagenesis Studies of Myoglobin Dynamics....Pages 347-354
Comparison of the Secondary Structures of Human Class I and Class II MHC Antigens by Ftir and CD Spectroscopy....Pages 355-365
Specificities of Germ Line Antibodies....Pages 367-376
Back Matter....Pages 377-385