Protein Phosphorylation Part B: Analysis of Protein Phosphorylation, Protein Kinase Inhibitors, and Protein Phosphatases

Content: 
Contributors to volume 201
Pages ix-xii

Preface
Pages xiii-xiv
Bartholomew M. Sefton, Tony Hunter

Volumes in series
Pages xv-xxxi

[1] Separation of phosphotyrosine, phosphoserine, and phosphothreonine by high-performance liquid chromatography Original Research Article
Pages 3-10
David P. Ringer

[2] Chemical properties and separation of phosphoamino acids by thin-layer chromatography and/or electrophoresis Original Research Article
Pages 10-21
Bertrand Duclos, Sylvie Marcandier, Alain J. Cozzone

[3] Determination of phosphoamino acid composition by acid hydrolysis of protein blotted to immobilon Original Research Article
Pages 21-27
Mark P. Kamps

[4] Comparison of three methods for detecting tyrosine-phosphorylated photeins Original Research Article
Pages 28-43
Lynn M. Kozma, Anthony J. Rossomando, Michael J. Weber

[5] Generation and use of antibodies to phosphothreonine Original Research Article
Pages 44-53
Daphna Heffetz, Mati Fridkin, Yehiel Zick

[6] Generation and use of anti-phosphotyrosine antibodies raised against bacterially expressed abl protein Original Research Article
Pages 53-65
Jean Y.J. Wang

[7] Preparation and use of anti-phosphotyrosine antibodies to study structure and function of insulin receptor Original Research Article
Pages 65-79
Morris F. White, Jonathan M. Backer

[8] Generation of monoclonal antibodies against phosphotyrosine and their use for affinity purification of phosphotyrosine-containing proteins Original Research Article
Pages 79-92
A.Raymond Frackelton Jr., M. Posner, B. Kannan, F. Mermelstein

[9] Isolation of tyrosine-phosphorylated proteins and generation of monoclonal antibodies Original Research Article
Pages 92-100
John R. Glenney

[10] Generation and use of anti-phosphotyrosine antibodies for immunoblotting Original Research Article
Pages 101-110
Mark P. Kamps

[11] Phosphopeptide mapping and phosphoamino acid analysis by two-dimensional separation on thin-layer cellulose plates Original Research Article
Pages 110-149
William J. Boyle, Peter van der Geer, Tony Hunter

[12] Cyanogen bromide cleavage and proteolytic peptide mapping of proteins immobilized to membranes Original Research Article
Pages 149-152
Kunxin Luo, Tamara Rhurley, Bartholomew M. Sefton

[13] Analysis of the in vivo phosphorylation states of proteins by fast atom bombardment mass spectrometry and other techniques Original Research Article
Pages 153-168
Philip Cohen, Bradford W. Gibson, Charles F.B. Holmes

[14] Determination and location of phosphoserine in proteins and peptides by conversion to S-ethylcysteine Original Research Article
Pages 169-185
Helmut E. Meyer, Edeltraut Hoffmann-Posorske, Ludwig M.G. Heilmeyer Jr.

[15] Solid-phase sequencing of 32P-labeled phosphopeptides at picomole and subpicomole levels Original Research Article
Pages 186-199
Richard E.H. Wettenhall, Ruedi H. Aebersold, Leroy E. Hood

[16] Identification of phosphorylation sites in peptides using a microsequencer Original Research Article
Pages 200-206
Peter T. Roach, Yuhuan Wang

[17] Sequence analysis of phosphotyrosine-containing peptides Original Research Article
Pages 206-224
Helmut E. Meyer, Edeltraut Hoffmann-Posorske, Arianna Donella-Deana, Horst Korte

[18] Synthesis of O-phosphoserine- and O-phosphothreonine-containing peptides Original Research Article
Pages 225-233
John W. Perich

[19] Synthesis of O-phosphotyrosine-containing peptides Original Research Article
Pages 234-245
John W. Perich

[20] Measurement of stoichiometry of protein phosphorylation by biosynthetic labeling Original Research Article
Pages 245-251
Bartholomew M. Sefton

[21] Estimation of phosphorylation stoichiometry by separation of phosphorylated isoforms Original Research Article
Pages 251-261
Jonathan Acooper

[22] Microchemical determination of phosphate in proteins isolated from polyacrylamide gels Original Research Article
Pages 261-264
Victor Dvacquier, Gary W. Moy

[23] Production of phosphorylation state-specific antibodies Original Research Article
Pages 264-283
Andrew Jczernik, Jean-Antoine Girault, Angus C. Nairn, Jonathan Chen, Gretchen Snyder, John Kebabian, Paul Greengard

[24] Pseudosubstrate-based peptide inhibitors Original Research Article
Pages 287-304
Bruce E. Kemp, Richard B. Pearson, Colin M. House

[25] Utilization of the inhibitor protein of adenosine cyclic monophosphate-dependent protein kinase, and peptides derived from it, as tools to study adenosine cyclic monophosphate-mediated cellular processes Original Research Article
Pages 304-316
Donal Awalsh, David B. Glass

[26] Use of sphingosine as inhibitor of protein kinase C Original Research Article
Pages 316-328
Yusuf A. Hannun, Alfred H. Merrill Jr., Robert M. Bell

[27] Properties and use of H-series compounds as protein kinase inhibitors Original Research Article
Pages 328-339
Hiroyoshi Hidaka, Masato Watanabe, Ryoji Kobayashi

[28] Use and specificity of staurosporine, UCN-O1, and calphostin C as protein kinase inhibitors Original Research Article
Pages 340-347
Tatsuya Tamaoki

[29] Inhibition of protein-tyrosine kinases by tyrphostins Original Research Article
Pages 347-361
Alexander Levitzki, Aviv Gazit, Nir Osherov, Israel Posner, Chaim Gilon

[30] Use and specificity of genistein as inhibitor of protein-tyrosine kinases Original Research Article
Pages 362-370
Tetsu Akiyama, Hiroshi Ogawara

[31] Use and selectivity of herbimycin A as inhibitor of protein-tyrosine kinases Original Research Article
Pages 370-379
Yoshimasa Uehara, Hidesuke Fukazawa

[32] Use of erbstatin as protein-tyrosine kinase inhibitor Original Research Article
Pages 379-385
Kazuo Umezawa, Masaya Imoto

[33] Classification of protein- serine/threonine phosphatases: identification and quantitation in cell extracts Original Research Article
Pages 389-398
Philip Cohen

[34] Cloning of protein- serine/threonine phosphatases Original Research Article
Pages 398-408
Patricia T.W. Cohen

[35] Reactivation of protein phosphatase 1 expressed at high levels from recombinant baculovirus Original Research Article
Pages 408-414
Patricia T.W. Cohen, Norbert Berndt

[36] Targeting subunits for protein phosphatases Original Research Article
Pages 414-427
Michael J. Hubbard, Philip Cohen

[37] Purification of protein-tyrosine phosphatases from human placenta Original Research Article
Pages 427-442
Nicholas K. Tonks, Curtis D. Diltz, Edmond H. Fischer

[38] Purification and assay of CD45: An integral membrane protein-tyrosine phosphatase Original Research Article
Pages 442-451
Nicholas K. Tonks, Curtis D. Diltz, Edmond H. Fischer

[39] Resolution and characterization of multiple protein-tyrosine phosphatase activities Original Research Article
Pages 451-465
Thomas S. Ingebritsen

[40] Use of okadaic acid to inhibit protein phosphatases in intact cells Original Research Article
Pages 469-476
D.Grahame Hardie, Timothy A.J. Haystead, Alistair T.R. Sim

[41] Use of vanadate as protein-phosphotyrosine phosphatase inhibitor Original Research Article
Pages 477-482
Julius A. Gordon

Author index
Pages 483-504

Subject index
Pages 505-547