Protein Engineering Handbook [2 vols] [biochem]

Front Matter (pages I–XLI)......Page 1
Guidelines for the Functional Analysis of Engineered and Mutant Enzymes (pages 1–13)......Page 42
Engineering Enantioselectivity in Enzyme-Catalyzed Reactions (pages 15–46)......Page 55
Mechanism and Catalytic Promiscuity Emerging Mechanistic Principles for Identification and Manipulation of Catalytically Promiscuous Enzymes (pages 47–79)......Page 87
Φ-Value Analysis of Protein Folding Transition States (pages 81–120)......Page 120
Protein Folding and Solubility Pathways and High-Throughput Assays (pages 121–145)......Page 160
Protein Dynamics and the Evolution of Novel Protein Function (pages 147–185)......Page 185
Gaining Insight into Enzyme Function through Correlation with Protein Motions (pages 187–211)......Page 224
Structural Frameworks Suitable for Engineering (pages 213–232)......Page 249
Microbes and Enzymes Recent Trends and New Directions to Expand Protein Space (pages 233–269)......Page 269
Inteins in Protein Engineering (pages 271–293)......Page 306
From Prospecting to Product – Industrial Metagenomics Is Coming of Age (pages 295–323)......Page 329
Computational Protein Design (pages 325–342)......Page 358
Assessing and Exploiting the Persistence of Substrate Ambiguity in Modern Protein Catalysts (pages 343–362)......Page 376
Designing Programmable Protein Switches (pages 363–389)......Page 396
The Cyclization of Peptides and Proteins with Inteins (pages 391–407)......Page 423
A Method for Rapid Directed Evolution (pages 409–439)......Page 440
Evolution of Enantioselective Bacillus subtilis Lipase (pages 441–451)......Page 471
Circular Permutation of Proteins (pages 453–471)......Page 482
Incorporating Synthetic Oligonucleotides via Gene Reassembly (ISOR) A Versatile Tool for Generating Targeted Libraries (pages 473–480)......Page 501
Protein Engineering by Structure-Guided SCHEMA Recombination (pages 481–492)......Page 509
Chimeragenesis in Protein Engineering (pages 493–514)......Page 521
Protein Generation Using a Reconstituted System (pages 515–535)......Page 543
Equipping in vivo Selection Systems with Tunable Stringency (pages 537–561)......Page 564
Protein Engineering by Phage Display (pages 563–603)......Page 589
Screening Methodologies for Glycosidic Bond Formation (pages 605–620)......Page 630
Yeast Surface Display in Protein Engineering and Analysis (pages 621–648)......Page 646
In Vitro Compartmentalization (IVC) and Other High-Throughput Screens of Enzyme Libraries (pages 649–667)......Page 674
Colorimetric and Fluorescence-Based Screening (pages 669–711)......Page 693
Confocal and Conventional Fluorescence-Based High Throughput Screening in Protein Engineering (pages 713–751)......Page 736
Alteration of Substrate Specificity and Stereoselectivity of Lipases and Esterases (pages 753–775)......Page 775
Altering Enzyme Substrate and Cofactor Specificity via Protein Engineering (pages 777–796)......Page 798
Protein Engineering of Modular Polyketide Synthases (pages 797–827)......Page 818
Cyanophycin Synthetases (pages 829–848)......Page 849
Biosynthetic Pathway Engineering Strategies (pages 849–876)......Page 869
Natural Polyester-Related Proteins Structure, Function, Evolution and Engineering (pages 877–914)......Page 897
Bioengineering of Sequence-Repetitive Polypeptides Synthetic Routes to Protein-Based Materials of Novel Structure and Function (pages 915–938)......Page 935
Silk Proteins – Biomaterials and Bioengineering (pages 939–959)......Page 959
Index (pages 961–973)......Page 980