Protein Engineering

Content: 
Editors-In-Chief
Page ii

Contributors to Volume 388
Pages ix-xii

Preface
Pages xiii-xiv

Methods In Enzymology
Pages xv-xxxvii

Gene Site Saturation Mutagenesis: A Comprehensive Mutagenesis Approach Original Research Article
Pages 3-11
Keith A Kretz, Toby H Richardson, Kevin A Gray, Dan E Robertson, Xuqiu Tan, Jay M Short

Single-Stranded DNA Family Shuffling Original Research Article
Pages 11-21
Osamu Kagami, Miho Kikuchi, Shigeaki Harayama

Module Shuffling Original Research Article
Pages 22-34
Satoshi Kaneko

SCHEMA-Guided Protein Recombination Original Research Article
Pages 35-42
Jonathan J Silberg, Jeffrey B Endelman, Frances H Arnold

Staggered Extension Process In Vitro DNA Recombination Original Research Article
Pages 42-49
Huimin Zhao

Using Incremental Truncation to Create Libraries of Hybrid Enzymes Original Research Article
Pages 50-60
Alexander R Horswill, Todd A Naumann, Stephen J Benkovic

Random Multirecombinant Polymerase Chain Reaction Original Research Article
Pages 61-75
Toru Tsuji, Michiko Onimaru, Masanobu Kitagawa, Kanehisa Kojoh, Noriko Tabata, Hiroshi Yanagawa

Gene Library Synthesis by Structure-Based Combinatorial Protein Engineering Original Research Article
Pages 75-91
Paul E. O\'Maille, Ming-Daw Tsai, Bryan T. Greenhagen, Joseph Chappell, Joseph P. Noel

New Enzymes from Combinatorial Library Modules Original Research Article
Pages 91-102
Werner Besenmatter, Peter Kast, Donald Hilvert

Construction of Protein Fragment Complementation Libraries Using Incremental Truncation Original Research Article
Pages 103-116
David E Paschon, Marc Ostermeier

GigaMatrix: A Novel Ultrahigh Throughput Protein Optimization and Discovery Platform Original Research Article
Pages 119-134
Mike Lafferty, Mark J Dycaico

High Throughput Microplate Screens for Directed Protein Evolution Original Research Article
Pages 134-145
Melissa L Geddie, Lori A Rowe, Omar B Alexander, Ichiro Matsumura

Periplasmic Expression as a Basis for Whole Cell Kinetic Screening of Unnatural Enzyme Reactivities Original Research Article
Pages 145-156
Grazyna E Sroga, Jonathan S Dordick

Engineering the Thermotolerance and pH Optimum of Family 11 Xylanases by Site-Directed Mutagenesis Original Research Article
Pages 156-167
Ossi Turunen, Janne Jänis, Fred Fenel, Matti Leisola

Screening for Oxidative Resistance Original Research Article
Pages 167-175
Joel R Cherry, Michael H Lamsa

Consensus-Based Engineering of Protein Stability: From Intrabodies to Thermostable Enzymes Original Research Article
Pages 176-186
Boris Steipe

Improving the Functional Expression of N-Carbamoylase by Directed Evolution using the Green Fluorescent Protein Fusion Reporter System Original Research Article
Pages 187-195
Hee-Sung Park, Ki-Hoon Oh, Hak-Sung Kim

Directed Evolution of Lipases and Esterases Original Research Article
Pages 199-207
Marlen Schmidt, Markus Baumann, Erik Henke, Monika Konarzycka-Bessler, Uwe T Bornscheuer

Protein Engineering of the Cytochrome P450 Monooxygenase from Bacillus megaterium Original Research Article
Pages 208-224
Vlada B Urlacher, Rolf D Schmid

Directed Evolution of Aldolases Original Research Article
Pages 224-238
Dirk Franke, Che-Chang Hsu, Chi-Huey Wong

Changing the Enantioselectivity of Enzymes by Directed Evolution Original Research Article
Pages 238-256
Manfred T Reetz

Control of Stereoselectivity in Phosphotriesterase Original Research Article
Pages 256-266
Suk-Bong Hong, Frank M Raushel

Manipulation and Analysis of Polyketide Synthases Original Research Article
Pages 269-293
Pawan Kumar, Chaitan Khosla, Yi Tang

Reactions Catalyzed by Mature and Recombinant Nonribosomal Peptide Synthetases Original Research Article
Pages 293-315
Uwe Linne, Mohamed A Marahiel

Engineering Carotenoid Biosynthetic Pathways Original Research Article
Pages 315-329
Benjamin N Mijts, Pyung C Lee, Claudia Schmidt-Dannert

Directed Evolution of Bacteriorhodopsin for Device Applications Original Research Article
Pages 333-347
Jason R Hillebrecht, Kevin J Wise, Jeremy F Koscielecki, Robert R Birge

Engineering Antibody Affinity by Yeast Surface Display Original Research Article
Pages 348-358
David W Colby, Brenda A Kellogg, Christilyn P Graff, Yik A Yeung, Jeffrey S Swers, K.Dane Wittrup

A Conditionally Replicating Virus as a Novel Approach Toward an HIV Vaccine Original Research Article
Pages 359-379
Atze T Das, Koen Verhoef, Ben Berkhout

Author Index
Pages 381-404

Subject Index
Pages 405-413