Protein Crosslinking: Biochemical and Molecular Aspects

Front Matter....Pages i-xix
Biologically Important Thiol-Disulfide Reactions and the Role of Cyst(E)Ine in Proteins: an Evolutionary Perspective....Pages 1-30
Disulfide Crosslinks and the Specificity of Protein Turnover in Plants....Pages 31-42
Protein Thiol-Disulfide Interchange and Interfacing with Biological Systems....Pages 43-50
On the Mechanism of Renaturation of Proteins Containing Disulfide Bonds....Pages 51-65
Disulfide Bonds: Key to Wheat Protein Functionality....Pages 67-88
Chemical Strategy for Studying the Antigenic Structures of Disulfide-Containing Proteins: Hen Egg-White Lysozyme as a Model....Pages 89-137
Crosslinking of Antibody Molecules by Bifunctional Antigens....Pages 139-148
Modification of the Biological Properties of Plant Lectins by Chemical Crosslinking....Pages 149-168
Introduction of Artificial Crosslinks into Proteins....Pages 169-186
Synthesis and Application of New Bifunctional Reagents....Pages 187-195
Synthesis and Application of Cleavable and Hydrophilic Crosslinking Reagents....Pages 197-206
Comparison of Hydrophobic and Strongly Hydrophilic Cleavable Crosslinking Reagents in Intermolecular Bond Formation in Aggregates of Proteins or Protein-RNA....Pages 207-214
Crosslinking of Ribosomes by Cleavable Bifunctional Mercaptoimidates....Pages 215-233
On the Introduction of Disulfide Crosslinks into Fibrous Proteins and Bovine Serum Albumin....Pages 235-245
Thiolation and Disulphide Cross-Linking of Insulin to Form Macromolecules of Potential Therapeutic Value....Pages 247-260
Crosslinked Insulins: Preparation, Properties, and Applications....Pages 261-282
The Enzymic Derivation of Citrulline Residues from Arginine Residues In Situ during the Biosynthesis of Hair Proteins that are Cross-Linked by Isopeptide Bonds....Pages 283-294
Thermodynamics of Cross Links....Pages 295-306
Physical and Chemical Consequences of Keratin Crosslinking, with Application to the Determination of Crosslink Density....Pages 307-327
An X-Ray Diffraction Study of Thermally-Induced Structural Changes in α-Keratin....Pages 329-344
Introduction of New Crosslinks into Proteins....Pages 345-354
Comparison of Wool Reactions with Selected Mono- and Bifunctional Reagents....Pages 355-382
The Effects of Ethylene Glycol on Wool Fibers....Pages 383-390
Protein: Polyanion Interactions. Studies on the Trehalose-P Synthetase as a Model System....Pages 391-404
Kinetic Studies of Immobilized α-Chymotrypsin in Apolar Solvents....Pages 405-413
Factors Affecting Cyanoborohydride Reduction of Aromatic Schiff’s Bases in Proteins....Pages 415-424
Chemistry of the Crosslinking of Collagen during Tanning....Pages 425-440
Chemical Modification of Collagen and the Effects on Enzyme-Binding: Mechanistic Considerations....Pages 441-471
Strategies in the Racemization-Free Synthesis of Polytripeptide Models of Collagen....Pages 473-490
Conformational Properties of Polypeptide Models of Collagen....Pages 491-507
Ionizing Radiation-Induced Crosslinking in Proteins....Pages 509-547
Peroxydisulfate Anion-Induced Crosslinking of Proteins....Pages 549-556
Cross Linking in the Radiolysis of Some Enzymes and Related Proteins....Pages 557-570
Isolation and Characterization of Stable Protein-DNA Adducts Induced in Chromatin by Ultraviolet Light....Pages 571-594
Identification of Binding Sites on the E. Coli Ribosome by Affinity Labeling....Pages 595-609
Photoinduced Nucleic Acid-Protein Crosslinkage in Ribosomes and Ribosome Complexes....Pages 611-631
Crosslinking of Nucleic Acids and Proteins by Bisulfite....Pages 633-640
Cross-Linking of Amino Acids by Formaldehyde. Preparation and 13 C NMR Spectra of Model Compounds....Pages 641-647
Electron Microscopy of an Oligomeric Protein Stabilized by Polyfunctional Cross-Linking....Pages 649-656
Fish Myofibrillar Protein and Lipid Interaction in Aqueous Media as Detected by Isotope Labeling, Sucrose Gradient Centrifugation, Polyacrylamide Electrophoresis and Electron Paramagnetic Resonance....Pages 657-686
Gas-Liquid Chromatography and Mass Spectrometry of Lanthionine, Lysinoalanine, and S-Carboxyethylcysteine....Pages 687-712
Mass Spectra of Cysteine Derivatives....Pages 713-726
A Nuclear Magnetic Double Resonance Study of N-β-Bis-(β′-Chloroethyl) Phosphonylethyl-DL-Phenylalanine....Pages 727-743
Back Matter....Pages 745-760