Production of Biologicals from Animal Cells in Culture

Content: 
Front Matter, Page iii
Copyright, Page iv
Organizing Committee, Page v
Sponsors, Page vi
ESACT Executive Committee 1988–89, Page vii
10th ESACT Meeting, Avignon, France, Page viii
Chairman's statement, Pages ix-x, R.E. Spier
Introduction, Pages xi-xii, R.E. Spier, J.B. Griffiths, B. Meignier
ANIMAL CELL CULTURE: THE PROBLEMS AND REWARDS, Pages 3-12, N B Finter
THE ISOLATION OF IMMORTAL MURINE MACROPHAGE CELL LINES, Pages 15-17, Ute Kreuzburg, Caroline MacDonald
PROPAGATION OF HUMAN CD4-IMMUNOGLOBULIN PRODUCING CELL LINES, Pages 18-27, E.-J. Schlaeger, B. Schumpp
CHARACTERISATION OF CELL BANKS BY DNA FINGERPRINTING, Pages 28-33, G.N. Stacey, S. Lanham, S.J. Booth, B.J. Bolton
EVALUATING THE SAFETY OF MURINE AND HUMAN HYBRIDOMAS: NEW PROBLEMS AND NEW TECHNIQUES, Pages 34-38, David Onions, Gillian Lees
DEFECTIVE ENDOGENOUS RETROVIRUSLIKE PARTICLES OF CHINESE HAMSTER OVARY CELLS, Pages 39-45, Kevin P. Anderson, Mari-Anne L. Low, Yolanda S. Lie, Richard Lazar, Gilbert Keller, Marshall Dinowitz
REFERENCE CELL LINES, Pages 46-47, R Bliem, JB Griffiths
A EUROPEAN DATABASE FOR ANIMAL CELL LINES, Page 48, A Doyle, J B Griffiths, C Caulcott
CELL LINE DATA BASE: AN ON-LINE DATA BANK WHERE INDUSTRY MEETS RESEARCHERS, Pages 49-51, Ottavia Aresu, Beatrice Iannotta, Barbara Parodi, Paolo Romano, Tiziana Ruzzon
ADHESION AND MOTILITY OF EMBRYONIC AND CANCER CELLS, Pages 52-54, J.P. Thiery, J.L Duband, S. Dufour, B. Boyer, G.C. Tucker, A.M. Valles, J. Gavrilovic, G. Moens, J. Jouanneau
IMMORTALIZATION OF CELLS WITH ONCOGENES, Pages 55-57, Jacques SAMARUT
LIPID METABOLISM OF ANIMAL CELLS IN CULTURE - A REVIEW, Pages 61-66, Georg Schmid
LIPOPROTEIN REQUIREMENTS OF CULTURED MAMMALIAN CELLS, Pages 67-69, Guy Hewlett
EFFECT OF NUTRIENTS AND WASTE METABOLITES ON GROWTH, METABOLISM AND PROTEIN SYNTHESIS IN HYBRIDOMA, Pages 70-72, Mohamed Al-Rubeai, A N Emery
HYBRIDOMA GROWTH, METABOLISM, AND PRODUCT FORMATION IN HEPES-BUFFERED MEDIUM: EFFECT OF PASSAGE NUMBER AND pH, Pages 73-75, Georg Schmid, Harvey W. Blanch, Charles R. Wilke
A SCREENING EXPERIMENT OF MEDIA SUPPLEMENTS ON CHO CELLS, Pages 76-78, Carol Gebert, Peter P. Gray
ENERGETICS OF GLUTAMINOLYSIS - A THEORETICAL EVALUATION, Pages 79-81, Lena Häggström
DEGRADING EFFECT OF LIGHT ON CELL CULTURE MEDIA, Pages 82-84, Hans Ingolf Nielsen, Kjell Bertheussen
CULTURE OF HYBRIDOMA IN DIALYSED MEDIA, Pages 85-89, C. MAZARS, L. POUGET, B. PINGEON, S. PROVENCHERE, ET P. HENNO
EVALUATION OF FETAL BOVINE SERUM SUBSTITUTES ON CELL GROWTH AND RECOMBINANT PROTEIN PRODUCTION, Pages 90-94, Luciano Ramos, Melvin S. Oka, Amy A. Murnane
CELL GROWTH ACTIVATORS (CGA) AND CELL FUNCTION MODULATORS (CFM) OF BLOOD: EFFECT ON CELL REPLICATION AND PROTEIN EXPRESSION, Pages 95-103, S.K. Dinka, P. Dehazya, J.S. Dinka, P. Gariepy, E. Szekely, P. Barber
INCREASED EXPRESSION OF FACTOR VIII BY BUTYRATE IN CHINESE HAMSTER OVARY CELLS, Pages 104-106, V. GANNE, P. GUERIN, T. FAURE, G. MIGNOT
POLYAMINE ENHANCED PRODUCT EXPRESSION FROM TRANSFORMED AND RECOMBINANT CELL LINES, Pages 107-109, Stephen J. Froud, John McLean, Andrew Mint
THE DEVELOPMENT OF A PROCESS FOR THE PRODUCTION OF HIV1 GP120 FROM RECOMBINANT CELL LINES, Pages 110-116, S.J. Froud, G.J. Clements, M.E. Doyle, E.L.V. Harris, C. Lloyd, P. Murray, A. Preneta, P.E. Stephens, S. Thompson, G.T. Yarranton
“SAFE” ANIMAL DERIVED MEDIUM SUPPLEMENTS FOR CELL CULTURES, Pages 117-124, Adolf Von Seefried, Paul Haffenden
EFFECT OF DESFERRIOXAMINE ON TRANSFERRIN RECEPTOR EXPRESSION, GROWTH & IgG PRODUCTION OF THE RAT HYBRIDOMA MA C57, Pages 125-129, N.J. Tomlinson, L. Davis, J. Craig-Gray, N. Jenkins
DEVELOPMENT OF SERUM-FREE MEDIA FOR MAMMALIAN CELL CULTURE, Pages 133-136, J. Kierulff, B. Persson, P. Rexen, C. Morcel, C. Emborg
CONCEPT AND STRATEGY FOR THE DEVELOPMENT OF A GENERAL PURPOSE SERUM-FREE MEDIUM FOR ANCHORAGE-DEPENDENT CELLS, Pages 137-139, L. Kaspi, D. Fiorentini, R. Talmon, R. Levi, G. Bennett
GROWTH OF MAMMALIAN CELL LINES UNDER SERUM-FREE CONDITIONS, Pages 140-143, Bente Rasmussen, Claus Koch
DEVELOPMENT OF A LOW-SERUM GROWTH MEDIUM FOR PRODUCTION OF A HUMAN GLYCOPROTEIN BY RECOMBINANT BHK CELLS, Pages 144-147, G. Schmid, H. Zilg, R. Johannsen
OPTIMALISATION OF A SERUM FREE MEDIUM FOR LARGE SCALE CONTINUOUS GROWTH AND IMMUNOGLOBULIN PRODUCTION BY A MOUSE HYBRIDOMA, Page 148, Kristin Heirwegh, Toon De Kesel, Peter De Waele
Longterm Serum-Free Culture of Murine Hybridomas, Pages 149-154, S.A. Clark, A.J. Racher, J.B. Griffiths
Serum-free media suitable for upstream and downstream processing, Pages 155-166, Volker Jäger
PRODUCTION OF HUMAN FIBRONECTIN FROM SERUM- FREE CONTINUOUS CULTURED HUMAN HEPATOBLASTOMA CELL LINE, HUH-6, Pages 167-169, K. Nagamine, M. Shiraishi, Z. Kong, K. Shinohara, H. Murakami
DEVELOPMENT OF A PROTEIN-FREE MEDIUM FOR BIOREACTOR CULTURES OF HYBRIDOMA CELL LINES, Pages 170-179, O-W Merten, H Keller, L Cabanié
CONDITIONS FOR THE PRODUCTION OF RECOMBINANT IL-2 IN STIRRED SUSPENSION CULTURE USING A PROTEIN FREE MEDIUM, Pages 180-186, Mona Lucki-Lange, Roland Wagner
A PROTEIN-FREE SERUM REPLACEMENT (SSR) FOR CELL CULTURE, Pages 187-189, Hans Ingolf Nielsen, Kjell Bertheussen
Adaptation and Characterization of Hybridomas Growing in Low Protein and Protein-Free Medium, Pages 190-195, Frieder Hofmann, Kathie Fritchman
CHARACTERIZATION OF PROTEASE ACTIVITY IN SERUM-FREE CULTURE SUPERNATANTS OF HYBRIDOMAS AND RECOMBINANT MAMMALIAN CELLS, Pages 196-203, Waldemar Lind, Volker Jäger, Mona Lucki-Lange, Roland Wagner
ANIMAL CELLS IN CULTURE MAKE SECONDARY METABOLITES TOO, Pages 207-217, R.E. Spier
REMOVAL OF INHIBITORY FACTORS FROM HYBRIDOMA CELL CULTURES BY GEL FILTRATION, Pages 218-225, øystein W. Rønning, Mona Schartum
THE EFFECT OF METABOLIC BY-PRODUCTS ON ANIMAL CELLS IN CULTURE, Pages 226-228, Michael Butler, Thomas Hassell, Christopher Doyle, Susan Gleave, Philip Jennings
CYTOSKELETAL MICROFILAMENT NETWORK AND ENERGY METABOLISM AFFECT ABILITY OF ANIMAL CELLS TO RESIST SHEAR INJURY, Pages 229-235, E. Terry Papoutsakis, J.F. Petersen, L.V. McIntiret
INTRACELLULAR CONCENTRATION OF ATP AND OTHER NUCLEOTIDES DURING CONTINUOUS CULTIVATION OF HYBRIDOMA CELLS, Pages 236-243, Thomas Ryll, Volker Jäger, Roland Wagner
DETERMINATION OF THE “CRITICAL SHEAR STRESS LEVEL” FOR ADHERENT BHK CELLS, Pages 244-246, Gerlinde Kretzmer, Andreas Ludwig, Karl Schügerl
EFFECTS OF DISSOLVED OXYGEN SUPPLY AND GLUCOSE CONCENTRATION ON HUMAN LYMPHOBLASTOID CELL GROWTH AND METABOLISM IN CONTINUOUS CULTURE, Pages 247-255, F-X. DERAMOUDT, G. MIGNOT, O.W. MERTEN, X. DROUET
THE ROLE OF DISSOLVED OXYGEN IN GROWTH OF ANIMAL CELLS, Pages 256-258, Caroline DEMANGEL, Dominique DUVAL, Isabelle GEAHEL
THE EFFECT OF OSMOLARITY ON HYBRIDOMA CELL GROWTH AND ANTIBODY PRODUCTION IN SERUM-FREE MEDIA, Pages 259-261, C.L. Marshall, R. Boraston, M.E. Brown
PHYSIOLOGIGAL INVESTIGATIONS IN HIGH DENSITY PERFUSION CULTURE OF FREE SUSPENDED ANIMAL CELLS, Pages 262-265, C. Fenge, E. Fraune, K. Schügerl
FACTORS AFFECTING HYBRIDOMA CELL ATTACHMENT TO DERIVATIZED POLYACRYLAMIDE BEADS, Pages 266-275, Y. Kaplan, A. Freeman
ADAPTATION OF ANCHORAGE-DEPENDENT CELLS TO GLUTAMINE-FREE MEDIUM, Pages 276-278, R.H. Thomas, H.A. Jenkins, M. Butler
The 2′5′oligoadenylate synthetase, a mutienzyme system involved in the growth properties and the sensitivity to viruses of mammalian cells: selection of antibodies in order to investigate their specific biological roles, Pages 279-284, S. Lowagie, A. Herrera, E. Sexsmith, V. Chotteau, E. Ledent, B. Williams, J. Wérenne
Recent Developments in Mammalian Expression Systems, Pages 287-303, C.C. Hentschel
GENETIC ENGINEERING OF CELLULAR PHYSIOLOGY, Pages 304-308, S.L. Bell, C.R. Bebbington, M.E. Bushell, P.G. Sanders, M.F. Scott, R.E. Spier, J.N. Wardell
STABILITY OF AMPLIFIED DNA IN CHINESE HAMSTER OVARY CELLS, Pages 309-315, N.H. Cossons, P.M. Hayter, M.F. Tuite, N. Jenkins
METHOTREXATE AND CHO CELLS: PRODUCTIVITY AND GENETICS OF AMPLIFIED EXPRESSION VECTOR SEQUENCES, Pages 316-326, Florian M. Wurm, Vivek Bajaj, Wally Tanaka, Victor Fung, Amy Smiley, Adriana Johnson, Maria G. Pallavicini, Robert Arathoon
THE STABILITY OF EXPRESSION OF HYBRIDOMA CELLS IN HOMOGENEOUS CONTINUOUS CULTURE SYSTEMS, Pages 327-334, C.A.M.van der Velden-de Groot, J.M.Coco Martin, D.E. Martens, J.W. Oberink, E.C. Beuvery
CLONING AND EXPRESSION OF A BOVINE ANTI-TESTOSTERONE MONOCLONAL ANTIBODY, Pages 335-337, T. Jackson, B.A. Morris, P.G. Sanders
USE OF DICISTRONIC TRANSCRIPTION UNITS FOR THE CORRELATED EXPRESSION OF TWO GENES IN MAMMALIAN CELLS, Pages 338-344, Manfred Wirth, Lorin Schumacher, Hansjörg Hauser
LARGE-SCALE RECOMBINANT PROTEIN PRODUCTION USING THE INSECT CELLBACULOVIRUS EXPRESSION VECTOR SYSTEM: ANTISTASIN AND β-ADRENERGIC RECEPTOR, Pages 345-351, D. Jain, K. Ramasubramanyan, S. Gould, A. Lenny, M. Candelore, M. Tota, C. Strader, K. Alves, G. Cuca, J.S. Tung, G. Hunt, B. Junker, B.C. Buckland, M. Silberklang
QUANTITATION OF mRNA SPECIFIC FOR HEAVY AND LIGHT CHAINS OF IgG IN HYBRIDOMAS DURING DIFFERENT PHASES OF BATCH CULTURE, Pages 352-360, OTTO W MERTEN, MICHEL LENO, JEAN HACHE
DEVELOPMENT OF MAMMALIAN CELL MANUFACTURING PROCESSES, Pages 363-369, James Hope, Morris Rosenberg, William Tolbert
A NEW LOOK AT ANIMAL CELL BIOREACTOR DEVELOPMENT, Pages 370-380, Wei-Shou Hu, Matthew T. Scholz, Eric Favre, T. Craig Seamans
MAMMALIAN CELL FERMENTATION: Are specialized bioreactors a necessity?, Pages 381-384, Bo Persson, J. Kierulff, C. Emborg
ENGINEERING FOR STERILITY AND CONTAINMENT: DESIGN OF A 225L “POLYMODAL” FERMENTER FOR THE CONTAINMENT OF P3 PATHOGENS, Pages 387-389, Ross Cameron, Bryan Griffiths, Peter Hambleton
MONOCLONAL ANTIBODY PRODUCTION USING AN AIRLIFT FERMENTERSYSTEM CONSISTING OF A CONTINUOUS SEED FERMENTER AND A FED BATCH PRODUCTION FERMENTER, Pages 390-393, Kurt Konopitzky, Otto Kanzler, Katharina Windhab
BLEEDING OUT DEAD CELLS FROM FERMENTATION SYSTEMS, Pages 394-399, Torsten Björling, Ulf Malmström
HIGH INTENSITY GROWTH OF ADHERENT CELLS ON A POROUS CERAMIC MATRIX, Pages 400-406, D.W. Lee, J.R. Grace, P. Allardyce, D.G. Kilburn
CONSIDERATIONS IN THE DESIGN, DEVELOPMENT AND SCALE-UP OF GLASS BEAD PACKED REACTORS, Pages 407-415, R. Bliem, R. Oakley, V. Taiariol, K. Matsuoka, J. Long
ON-LINE REMOVAL OF CELLS FROM CONTINUOUS SUSPENSION CULTURES, Pages 416-420, Peter C. Brown, John C. Lane, Mark T. Wininger, Robert Chow
MICROSPHERE-INDUCED AGGREGATE CULTURE OF ANIMAL CELLS, Pages 423-428, Stephane Goetghebeur, Wei-Shou Hu
THE GROWTH OP CHO AND BHK CELLS AS SUSPENDED AGGREGATES IN SERUM-FREE MEDIUM, Pages 429-433, Jack Litwin
CULTISPHER - MACROPOROUS GELATIN MICROCARRIER - NEW APPLICATIONS, Pages 434-438, Joakim Almgren, Charlotte Nilsson, Ann-Christin Petersson, Kjell Nilsson
CULTIVATION OF CHO-CELLS ON MICROCARRIERS DORMACELL, Pages 439-442, M. Jacobs, G. Gummich, I. Keller, D. Schwengers
FERMENTATION OF AT III PRODUCING BHK CELLS IN A DOUBLE MEMBRANE PERFUSION BIOREAKTOR; some aspects about fermentation strategy and process optimization, Pages 445-450, Shu-ying Li, Bettina Röder, Manfred Wirth
OXYGEN TRANSFER CHARACTERISTICS IN CELL CULTURE FERMENTERS: DIRECT SPARGING - MEMBRANE OXYGENATION - BUBBLE-FREE AERATION THROUGH A ROTATING SIEVE, Pages 451-453, Thomas Thaler, Jan Vanàk
HYBRIDOMA CULTURE IN A MEMBRANE SYSTEM: THE INFLUENCE OF OXYGEN ON A REACTOR'S PERFORMANCE AND ON THE EVALUATION OF CELLULAR DENSITY, Pages 454-456, V. GEAUGEY, F. PASCAL, A. MARC, J.M. ENGASSER, D. DUVAL
HYBRIDOMA, ANTIBODY AND GROWTH FACTOR DISTRIBUTIONS IN THE SHELL-SIDE OF ULTRAFILTRATION HOLLOW FIBER BIOREACTORS, Pages 457-459, J.M. Piret, C.L. Cooney
CULTIVATION OF INSECT CELL LINES IN STIRRED MEMBRANE REACTORS, Pages 460-466, C. Schütz, V. Jäger, A.J. Driesel, R. Wagner
High Density Culture of Hybridoma Cells using a Perfusion Culture Apparatus with Multi-settling Zones, Pages 467-469, Michiyuki Tokashiki, Takami Arai
HIGH CELL DENSITY PERFUSION CULTURE OF INSECT CELLS FOR PRODUCTION OF BACULOVIRUS AND RECOMBINANT PROTEIN, Pages 470-476, Martina Klöppinger, Georg Fertig, Elisabeth Fraune, Herbert G. Miltenburger
METABOLITE PARAMETERS MODULATING MONOCLONAL ANTIBODY PRODUCTION BY HYBRIDOMA CELLS IN FLASK AND MACROPOROUS GLASS SPHERE CULTURE, Pages 479-485, AJ Racher, D Looby, JB Griffiths
OPTIMIZATION OF OKT3 HYBRIDOMA CULTURES IN A PERFUSION STIRRED REACTOR, Pages 486-488, H. PINTON, J.N. RABAUD, J.M. ENGASSER, A. MARC
BIOCHEMICAL CONTROL OF MONOCLONAL ANTIBODY SECRETION IN HOLLOW FIBRE BIOREACTORS, Pages 489-494, A. Handa-Corrigan, S. Nikolay, R.E. Spier
OPTIMIZATION OF HUMAN ANTI RHESUS IgG PRODUCTION USING HOLLOW FIBER TECHNOLOGY, Pages 495-497, F. DHAINAUT, L. POUGET, M.J. RICHER-HERS, G. MIGNOT
PARAMETERS FOR OPTIMISATION OF FERMENTATION OF AN AT III PRODUCING CELL LINE, Pages 498-501, U. Eberhard, G. Schmid, R. Johannsen
ENHANCED PRODUCTIVITY OF HYBRIDOMA AND RECOMBINANT CHO CELL CULTURES BY PLURONIC F-68 AND OTHER MEDIUM COMPONENTS, AND BY INCREASED PERFUSION RATES, IN FLUIDIZED-BED BIOREACTORS, Pages 502-512, N.G. Ray, A.S. Tung, P.W. Runstadler, J.N. Vournakis
Improved Performance of the Fluidized Bed Reactor for the Cultivation of Animal Cells, Pages 513-515, J. Keller, I.J. Dunn, E. Heinzle
BIOREACTORS FOR PRODUCTION OF HIV - A COMPARATIVE EVALUATION OF FLASK, SUSPENSION, AND FIXED BED CULTURES, Pages 519-522, J.B. Clarke, J.B. Griffiths
COMPARISON OF SEMICONTINUOUS CULTURE AND PERFUSION CULTURE IN A 75 L BIOREACTOR M. Hjertstedt,, Pages 523-524, F. Buzsaky, E. Lindner-Olsson
COMPARISON OF TWO SYSTEMS BASED ON POROUS BEADS FOR THE PRODUCTION OF RECOMBINANT VON WILLEBRAND FACTOR BY CHO CELLS, Pages 525-527, J. JANNIN, V. GASPARD, C. ZAOT, T. FAURE, F. DHAINAUT, G. MIGNOT
COMPARISON OF THE PRODUCTION EFFICIENCY OF MAMMALIAN CELLS GROWN IN A FLUIDIZED BED AND IN A STIRRED TANK BIOREACTOR, Pages 528-532, R. Kratje, V. Jäger, R. Wagner
MONOCLONAL ANTIBODY PRODUCTION IN THREE DIFFERENT HOLLOW FIBRE BIOREACTOR CONFIGURATIONS, Pages 533-536, S. Nikolay, A. Garcia de Castro, M. Chadd, A. Handa-Corrigan
A comparision of two reactors for cell cultivation, Pages 537-539, H. Rupp-Brunswig, W.-D. Deckwer
STAINING OF CHINESE HAMSTER OVARY CELLS ON MACROPOROUS MATRICES, Pages 543-547, Bruce Rubin, Luciano Ramos, Amy A. Murnane
The Role of Oxidation-Reduction Potential in Monitoring Growth of Cultured Mammalian Cells, Pages 548-568, Christopher Hwang, Anthony J. Sinskey
POTENTIAL AND PITFALLS OF USING LDH RELEASE FOR THE EVALUATION OF ANIMAL CELL DEATH KINETICS, Pages 569-575, A. MARC, A. WAGNER, A. MARTIAL, J.L. GOERGEN, J.M. ENGASSER, V. GEAUGEY, H. PINTON
FLOW CYTOMETRIC (FCM) ANALYSIS ON THE GENETIC STABILITY OF CONTINUOUS CELL LINES, Pages 576-579, G. Fertiq, M. Klöppinger, H.G. Miltenburger
BIOMASS ESTIMATION IN ANCHORAGE-DEPENDENT ANIMAL CELL CULTURES, Pages 580-588, Véronique CHOTTEAU, Luc FABRY, Serge LOWAGIE, John WERENNE
DYNAMIC ASSAY OF SYNTHETIC ACTIVITIES IN SINGLE HYBRIDOMA CELLS, Pages 589-593, Mohamed Al-Rubeai, S. Chalder, A.N. Emery
RAPID MONITORING OF MONOCLONAL ANTIBODIES IN CELL CULTURE MEDIA BY HIGH PERFORMANCE LIQUID AFFINITY CHROMATOGRAPHY (HPLAC), Pages 594-596, Ann Holmberg, Sten Ohlson, Torgny Lundgren
APPLICATION OF THE PCFIA TO RAPID OFF-LINE PROCESS MONITORING, Pages 597-599, E. JERVIS, D.W. LEE, D.G. KILBURN
MATHEMATICAL ESTIMATOR FOR THE EVALUATION OF CELL DENSITY AND MEDIUM COMPOSITION IN HYBRIDOMA CULTURES, Pages 603-605, E. Mailly, C. Fonteix, J.M. Engasser, A. Marc
KINETIC EFFECTS OF GROWTH FACTORS ON BATCH AND CONTINUOUS HYBRIDOMA CULTURES, Pages 606-608, A. MARTIAL, P. NABET, J.M. ENGASSER, A. MARC
BATCH PRODUCTION KINETICS OF HYBRIDOMAS: PULSE-EXPERIMENTS, Pages 609-615, O.-W. Merten, H. Keller, K. Siami, L. Cabanié, M. Leno
ANALYSIS OF HYBRIDOMA GROWTH, METABOLISM, AND PRODUCT FORMATION IN CONTINUOUS SUSPENSION CULTURE, Pages 616-621, G. Schmid, C.R. Wilke, H.W. Blanch
KINETICS OF NUTRIENT UTILIZATION IN A HYBRIDOMA BATCH CULTURE, Pages 622-624, H. Frieberg, L. Persson, KO Grönvik, L. Häggström
MODELLING GROWTH OF AND ANTIBODY PRODUCTION BY HYBRIDOMAS IN GLUTAMINE LIMITED SUSPENSION CULTURES, Pages 625-630, Lars Keld Nielsen, Wardono Niloperbowo, Steven Reid, Paul F. Greenfield
A COMPUTER SIMULATION OF THE KINETICS AND ENERGETICS OF HYBRIDOMA CELL GROWTH AND ANTIBODY PRODUCTION, Pages 631-633, John Barford, Colin Harbour
A KINETIC MODEL FOR THE INFLUENCE OF SERUM IN BATCH AND CONTINUOUS HYBRIDOMA CULTURES, Pages 634-636, J.L. GOERGEN, A. MARTIAL, A. MARC, J.M. ENGASSER
ISOLATION OF BIOLOGICALS LEACHABLES AND CLEANING IN PLACE BECOME A CRUCIAL ISSUE IN LIQUID CHROMATOGRAPHY, Pages 639-650, E. BOSCHETTI
COMPOSITE HIGH FLOW RATE ION EXCHANGERS FOR ECONOMICAL SCALE-UP, Pages 651-657, Magnus Glad, Stefan Schornack
SERUM FREE MEDIA (SFM) AND SERUM SUBSTITUTES (SS) IN ANIMAL CELL CULTURE: DOWNSTREAM CONSIDERATIONS, Pages 658-661, H. Graf, J.N. Rabaud, J.M. Egly
THE USE OF AFFINITY CHROMATOGRAPHY FOR THE PURIFICATION OF MONOCLONAL ANTIBODIES, Pages 662-665, H. Büntemeyer, D. Lütkemeyer, B. Bernat, J. Lehmann
Production of a pharmaceutical enzyme : Animal Cells or E.coli ?, Pages 669-694, Terence Cartwright, André Crespo, RHONE-POULENC SANTE
ORAL POLIO VACCINE (SABIN) PRODUCED ON LARGE-SCALE VERO CELL CULTURE, Pages 695-705, Bernard MONTAGNON, Bernard FANGET, Roger VINAS, Lucien PEYRON, Jean-Claude VINCENT-FALQUET, Pierre CAUDRELIER
STRATEGIES FOR IMPROVED PRODUCTION OF EPSTEIN-BARR VIRUS, Pages 706-708, A H Davies, J Huddelston, F J Evans, A B Rickinson, A N Emery
OPTIMAL CONDITIONS FOR THE GROWTH OF PIG KIDNEY ENDOTHELIAL CELLS (SK) PROLIFERATED ON MICROCARRIERS AND PRODUCTION OF PIG PARVOVIRUS (PPV) VACCINE, Pages 709-711, L. PORQUET, M. VAYREDA, C. AULINAS, J. PLANA DURAN
CONTINUOUS PRODUCTION OF A NOVEL THROMBOLYTIC FROM 50/100 LITRE PERFUSED MICROCARRIER CULTURES, Pages 712-714, C.J. Mannix, G.R. Pettman, W.A. Southwick
PRODUCTION OF RECOMBINANT HUMAN THYROTROPIN AND ITS INCOMPLETE PROCESSING IN CHO CELLS, Pages 715-717, Sachihiko Watanabe, Yoshinori Nishitani, Machiko Hirono, Hikaru Sonoda
Production of a monoklonal antibody against gelsolin, Pages 718-721, D. Lütkemeyer, H. Büntemeyer, J. Lehmann
APPEARANCE OF NONSPECIFIC AMOUNTS OF MONOCLONAL ANTIBODY DURING FERMENTATION CAUSED BY DECREASED CELL VIABILITY, Pages 722-725, U. Marx, V. Jäger, S.T. Kiessig, R. Grunow, R.v. Baehr
PRODUCTION OF BIOLOGICALLY ACTIVE INSULIN-LIKE GROWTH FACTOR BY CELLS TRANSFECTED WITH A RECOMBINANT RETROVIRAL VECTOR ENCODING IGF-1, Pages 726-731, Patricia Looby Watts, Caroline MacDonald
IN VITRO PRODUCTION OF GROWTH REGULATORS FOR HUMAN EPITHELIAL CELLS, Pages 732-734, Martin Clynes, Margaret Dooley, Breda Carey, Anne Godfrev, Angela O'Toole, Bernard Gregory, Eunan McGlinchey
PURIFICATION AND CHARACTERIZATION OF IMMUNOGLOBULIN PRODUCTION STIMULATING FACTOR IIα DERIVED FROM NAMALWA CELLS, Pages 735-741, Hiroki Murakami, Takuya Sugahara, Sanetaka Shirahata, Koji Yamada
PRODUCTION OF A CHIMERIC ANTIBODY FOR TUMOUR IMAGING AND THERAPY FROM CHINESE HAMSTER OVARY (CHO) AND MYELOMA CELLS, Pages 742-744, R.P. Field, H. Brand, G.L. Renner, H.A. Robertson, R. Boraston
OPTIMIZATION OF tPA PRODUCTION IN A 3 L CONTINUOUS PERFUSION BIOREACTOR, Pages 745-747, U. Källström, N. Chatzisavido, F. Buzsáky, E. Lindner-Olsson
THE PRODUCTION AND ASSAY OF tPA FROM MAMMALIAN CELLS, Pages 748-750, A.W. Grierson, C. Darnbrough, C. MacDonald
CONSTITUTIVE SECRETION OF SOLUBLE CD23 RECEPTORS IN HOLLOW FIBER REACTORS, Pages 751-753, A. Bernard, C. Cavegn, T. Jomotte, P. Graber, J.Y. Bonnefoy
PRODUCTION OF PROTEINS FROM CELL NUCLEI BY CYCLIC CONTINUOUS FERMENTATION, Pages 754-756, B. Röder, G.-W. Piehl, J. Lehmann
BIOLOGICALS AND REGULATORY ASPECTS IN THE E.E.C., Pages 759-774, Florian HORAUD
MAPPING OF N-LINKED OLIGOSACCHARIDES FROM HUMAN RECOMBINANT GLYCOPROTEINS: QUALITY CONTROL OF GLYCOSYLATED THERAPEUTIC POLYPEPTIDES, Pages 775-782, Harald S. Conradt, Karin Schaper, Christiane Proppe, Manfred Nimtz
VALIDATION OF DOWNSTREAM PROCESSING, Pages 783-788, Gillian Lees, David Onions
THE CULTURE ENVIRONMENT AFFECTS RECOMBINANT PROTEIN PROCESSING IN CHO CELLS, Pages 789-795, P. Hayter, E. Curling, A. Baines, A. Bull, I. Salmon, P. Strange, N. Jenkins
THE Medi-Cult® HYBRITEST - A NEW TEST FOR IN VITRO TOXICOLOGY, Pages 796-798, Hans Ingolf Nielsen, Kjell Bertheussen
List of exhibitors, Page 799
List of Participants, Pages 801-810
Subject index, Pages 811-821
Author index, Pages 823-826