Practical Plant Virology: Protocols and Exercises

Front Matter....Pages I-XVI
Front Matter....Pages 1-4
Mechanical Inoculation of Plants....Pages 5-13
Inoculation of Protoplasts Isolated from Leaves....Pages 14-28
Differential Temperature Treatment of Infected Plants....Pages 29-31
Infectivity of Tobacco Mosaic Tobamovirus ....Pages 32-33
Front Matter....Pages 35-41
Description of External Symptoms....Pages 42-47
Determination of Internal Symptoms....Pages 48-56
Systemic Acquired Resistance (SAR)....Pages 57-59
Demonstration of Phloem Necrosis in Plants Affected by Potato Leafroll....Pages 60-62
Inclusions in Hair Cells of Leaves of Tobacco Infected with Tobacco Mosaic Tobamovirus ....Pages 63-64
Front Matter....Pages 65-65
Infectivity Assay on Local-Lesion Hosts....Pages 67-77
Infectivity Assay on Hosts Producing Starch Lesions....Pages 78-81
Infectivity Assay on Hosts Producing Systemic Symptoms Only....Pages 82-85
Effect of Temperature on the Susceptibility of Plants....Pages 86-87
Effect of Abrasives on the Infection....Pages 88-89
Effect of Inhibitors on the Infection....Pages 90-91
Effect of Light on the Susceptibility of Plants....Pages 92-93
Effect of Time of the Day on the Susceptibility of Plants....Pages 94-95
Front Matter....Pages 97-99
Determination of the Dilution End-Point....Pages 100-101
Determination of the Thermal Inactivation Point....Pages 102-104
Determination of the Longevity In Vitro....Pages 105-107
Front Matter....Pages 97-99
Determination of the Dilution End-Point....Pages 108-109
Determination of the Thermal Inactivation Point....Pages 110-110
Determination of the Longevity In Vitro....Pages 111-111
Front Matter....Pages 113-115
Virus Transmission by Grafting....Pages 116-124
Virus Transmission Through Soil....Pages 125-127
Virus Transmission by Nematodes....Pages 128-142
Virus Transmission by Fungi....Pages 143-147
Virus Transmission by Aphids....Pages 148-158
Transmission by Leafhoppers (Cicadellidae)....Pages 159-168
Transmission by Whiteflies (Aleyrodidae)....Pages 169-173
Transmission by Thrips (Thripidae)....Pages 174-177
Wedge-Grafting of Tobacco....Pages 178-179
Separation of a Nonpersistently and a Persistently Transmitted Virus by Differential Transmission....Pages 180-183
Visualisation of Salivary Sheaths Formed by Aphids....Pages 184-185
Separation of a Semipersistently Transmitted and a Circulative-Propagative Virus by Differential Transmission....Pages 186-187
Front Matter....Pages 189-191
Freezing....Pages 192-193
Dehydration....Pages 194-197
Freeze-Drying....Pages 198-199
Short-Term Storage....Pages 200-201
Front Matter....Pages 203-206
Heat Treatment....Pages 207-208
Front Matter....Pages 203-206
Meristem-Tip Culture....Pages 209-215
Heat Treatment Combined With Meristem-Tip Culture....Pages 216-217
Front Matter....Pages 219-231
Density-Gradient Centrifugation....Pages 232-237
Virus Purification Protocols....Pages 238-273
Front Matter....Pages 275-275
UV Light Absorption....Pages 277-283
Buoyant Density in Caesium Chloride....Pages 284-290
Particle Morphology and Size as Determined with the Electron Microscope....Pages 291-297
Front Matter....Pages 299-301
Determination of the Molecular Mass of Coat Proteins by Gel Electrophoresis....Pages 302-307
RNA Extraction from Purified Virus Particles....Pages 308-311
Determination of the Molecular Mass of Ribonucleic Acids by Gel Electrophoresis....Pages 312-315
Front Matter....Pages 317-321
Production of Antiserum....Pages 322-328
Absorption of Antiserum....Pages 329-332
Chloroplast Agglutination Test....Pages 333-335
Microprecipitin Test....Pages 336-340
Gel Double-Diffusion Test in Plates....Pages 341-347
Enzyme-Linked Immunosorbent Assay....Pages 348-362
Immunoblotting Assays....Pages 363-372
Standard DAS-ELISA....Pages 373-375
Front Matter....Pages 377-379
Electron Microscope Serology....Pages 380-391
Light Microscope Serology....Pages 392-400
Front Matter....Pages 401-403
Northern Blotting....Pages 404-407
Northern Hybridisation....Pages 408-412
Dot-Blot Hybridisation (Nucleic Acid Spot Hybridisation)....Pages 413-414
Polymerase Chain Reaction....Pages 415-425
Diagnosis of a Disease and Identification of the Causal Virus(es)....Pages 427-434
Back Matter....Pages 435-459