Practical Methods in Cardiovascular Research

Front Matter....Pages 1-1
Anaesthesia (Inhalative or i.v. Narcosis) of Laboratory Animals....Pages 5-25
Extracorporeal Circulation....Pages 26-36
Open-chest Models of Acute Myocardial Ischemia and Reperfusion....Pages 37-57
Models of Chronic Ischemia and Infarction....Pages 58-71
Models on Left Ventricular Hypertrophy (LVH)....Pages 72-82
Experimental Models of Heart Failure....Pages 83-110
In-Vivo Models of Arrhythmias: a Canine Model of Sudden Cardiac Death....Pages 111-128
In-Vivo Models of Atrial Fibrillation....Pages 129-149
Front Matter....Pages 151-151
The Langendorff Heart....Pages 155-172
The Working Heart....Pages 173-189
Isolated Papillary Muscles....Pages 190-197
Isolated Vessels....Pages 198-211
Optical Techniques for the Recording of Action Potentials....Pages 215-232
Recording Action Potentials Using Voltage-Sensitive Dyes....Pages 233-255
Epicardial Mapping in Isolated Hearts — Use in Safety Pharmacology, Analysis of Torsade de Pointes Arrhythmia and Ischemia-Reperfusion-Related Arrhythmia....Pages 256-271
Voltage-Clamp and Patch-Clamp Techniques....Pages 272-323
L-Type Calcium Channel Recording....Pages 324-354
Recording Cardiac Potassium Currents with the Whole-Cell Voltage Clamp Technique....Pages 355-380
Recording the Pacemaker Current I f ....Pages 381-396
Recording Gap Junction Currents....Pages 397-416
Front Matter....Pages 151-151
Recording Monophasic Action Potentials....Pages 417-431
Heart on a Chip — Extracellular Multielectrode Recordings from Cardiac Myocytes in Vitro....Pages 432-453
Immunohistochemistry for Structural and Functional Analysis in Cardiovascular Research....Pages 457-484
Classical Histological Staining Procedures in Cardiovascular Research....Pages 485-499
Practical Use of in situ Hybridisation and RT in situ PCR in Cardiovascular Research....Pages 500-524
Practical Use of Transmission Electron Microscopy for Analysis of Structures and Molecules in Cardiovascular Research....Pages 525-545
DAF Technique for Real-Time NO Imaging in the Human Myocardium....Pages 546-554
Isolation and Culture of Adult Ventricular Cardiomyocytes....Pages 557-567
Culture of Neonatal Cardiomyocytes....Pages 568-576
Culture of Embryoid Bodies....Pages 577-591
Culturing and Differentiation of Embryonic and Adult Stem Cells for Heart Research and Transplantation Therapy....Pages 592-609
Preparation of Endothelial Cells from Microand Macrovascular Origin....Pages 610-629
In Vitro Cultivation of Vascular Smooth Muscle Cells....Pages 630-639
Engineering Heart Tissue for In Vitro and In Vivo Studies....Pages 640-658
High-Performance Liquid Chromatography (HPLC)....Pages 661-685
Protein Biochemistry....Pages 686-722
Receptor and Binding Studies....Pages 723-783
Recording and Analysing Concentration-Response Curves....Pages 784-798
Measurement of NO and Endothelial Function in Cardiovascular Research....Pages 799-812
Biomechanical Stimulation of Vascular Cells In Vitro ....Pages 813-828
Front Matter....Pages 151-151
Measurement of Function and Regulation of Adrenergic Receptors....Pages 829-847
Measurement of Function and Regulation of Muscarinic Acetylcholine Receptors....Pages 848-862
Cytometry in Cardiovascular Research....Pages 863-885
Front Matter....Pages 887-887
Real-Time PCR Analysis of Cardiac Samples....Pages 891-906
Use of cDNA Arrays to Explore Gene Expression in Genetically Manipulated Mice and Cell Lines....Pages 907-915
Using Antibody Arrays to Detect Protein-Protein Interactions....Pages 916-935
Surface Plasmon Resonance as a Method to Study the Kinetics and Amplitude of Protein- Protein Binding....Pages 936-947
How to Solve a Protein Structure by Nuclear Magnetic Resonance — The Connexin43 Carboxyl Terminal Domain....Pages 948-958
Managing a Transgenic Mouse Colony: A Guide for the Novice....Pages 959-975