Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices

Front Matter....Pages i-xxiii
Journey of Human Gametes In Vitro: 1978–2010....Pages 1-5
Front Matter....Pages 7-7
Building the Laboratory....Pages 9-15
Air Quality Management....Pages 17-25
Organizational Aspects of the Laboratory in a Tertiary Care ART Center....Pages 27-32
Quality Control Management....Pages 33-39
Daily, Weekly, and Regular Preparations for the IVF Laboratory....Pages 41-44
Risk and Safety in the IVF Clinic....Pages 45-51
Front Matter....Pages 53-53
Essential Instruments and Disposable Supplies for an IVF Laboratory....Pages 55-59
Co 2 and Low-O 2 Incubators....Pages 61-70
IVF Workstations....Pages 71-78
Culture Media in IVF: Decisions for the Laboratory....Pages 79-90
Front Matter....Pages 91-91
Oocyte Denuding....Pages 93-104
Assessment of Oocyte Quality....Pages 105-119
Polarization Microscopy....Pages 121-125
Cumulus Cell Gene Expression in Assessment of Oocyte Quality....Pages 127-130
Front Matter....Pages 131-131
Short Culture: Day 1/Day 2/Day 3 Embryo Culture....Pages 133-140
Extended Culture in IVF....Pages 141-150
In Vitro Maturation of Human Oocytes....Pages 151-160
In Vivo Embryo Culture Device....Pages 161-169
Microfluidics for Gamete Manipulation and Embryo Culture....Pages 171-181
Front Matter....Pages 183-183
Sperm Assessment: Traditional Approaches and Their Indicative Value....Pages 185-192
Sperm Assessment: Novel Approaches and Their Indicative Value....Pages 193-198
Sperm Processing for IVF....Pages 199-205
PESA/TESA/TESE Sperm Processing....Pages 207-220
Processing Sperm Samples in HIV-Positive Patients....Pages 221-228
Intracytoplasmic Morphologically Selected Sperm Injection....Pages 229-240
Sperm Testing and ICSI Selection by Hyaluronic Acid Binding: The Hyaluronic Acid-Coated Glass Slide and Petri Dish in the Andrology and IVF Laboratories....Pages 241-257
Electrophoretic Sperm Separation....Pages 259-263
Magnetic-Activated Cell Sorting of Human Spermatozoa....Pages 265-272
Polscope-Based Sperm Selection....Pages 273-277
Front Matter....Pages 279-279
Intrauterine Insemination....Pages 281-296
Conventional IVF Insemination....Pages 297-305
Intracytoplasmic Sperm Injection....Pages 307-320
Mechanism of Human Oocyte Activation During ICSI and Methodology for Overcoming Low or Failed Fertilization....Pages 321-326
Front Matter....Pages 327-327
Hydraulic Manipulators for ICSI....Pages 329-334
Research Instruments Micromanipulators....Pages 335-340
Eppendorf Micromanipulator: Setup and Operation of Electronic Micromanipulators....Pages 341-345
The Leica Microsystems’ IMSI System....Pages 347-352
Automated Robotic Intracytoplasmic Sperm Injection....Pages 353-359
Oocyte Treatment and Preparation for Microinjection....Pages 361-369
Front Matter....Pages 327-327
Livestock Production via Micromanipulation....Pages 371-375
Front Matter....Pages 377-377
Pronuclear Scoring in Human In Vitro Fertilization....Pages 379-384
Cumulative Morphological Assessment of Embryo Quality....Pages 385-404
Metabolomics: The ViaMetrics-E ™ Procedure for Assessing Embryo Viability....Pages 405-412
Oxygen Consumption as an Indicator of Oocyte and Embryo Viability....Pages 413-420
Gene Expression Changes During Human Early Embryo Development: New Applications for Embryo Selection....Pages 421-430
Amino Acid Turnover as a Biomarker of Embryo Viability....Pages 431-438
Real-Time Embryo Monitoring Device for Embryo Selection....Pages 439-444
Assisted Hatching in IVF....Pages 445-451
Front Matter....Pages 453-453
Polar Body Biopsy....Pages 455-459
Cleavage-Stage Embryo Biopsy....Pages 461-471
Embryo Biopsy for PGD: Current Perspective....Pages 473-481
Microarrays and CGH for PGD of Chromosome Abnormalities and Gene Defects....Pages 483-490
Front Matter....Pages 491-491
Sperm Cryopreservation....Pages 493-507
Slow Freezing of Oocytes....Pages 509-515
Vitrification: Research in Animal Models....Pages 517-521
Oocyte Vitrification....Pages 523-528
Slow Freezing of Embryos....Pages 529-538
Vitrification of Embryos....Pages 539-546
Directional Freezing of Reproductive Cells, Tissues, and Organs....Pages 547-550
Front Matter....Pages 491-491
Ovarian Tissue Cryopreservation....Pages 551-556
Front Matter....Pages 557-557
Assessment of Uterine Receptivity....Pages 559-566
Single Embryo Transfer....Pages 567-570
Ultrasound-Guided Embryo Transfer (Abdominal/Vaginal): An Evidence-Based Evaluation....Pages 571-574
Cumulus-Aided Embryo Transfer....Pages 575-580
Front Matter....Pages 581-581
Data Management in the ART Laboratory: Requirements and Solutions....Pages 583-592
Regulation, Licensing, and Accreditation of the ART Laboratory....Pages 593-604
Legislation in the United Kingdom....Pages 605-609
Regulation, Licensing, and Accreditation of the ART Laboratory in Europe....Pages 611-618
Regulation, Licensing, and Accreditation of ART Laboratories in India....Pages 619-630
Troubleshooting in the Clinical Embryology Laboratory: The Art of Problem-Solving in ART....Pages 631-637
Front Matter....Pages 639-639
The Role of Mitochondria in the Establishment of Developmental Competence in Early Human Development....Pages 641-657
Nuclear and Cytoplasmic Transfer: Human Applications and Concerns....Pages 659-665
Cytoskeletal Architecture of Human Oocytes with Focus on Centrosomes and Their Significant Role in Fertilization....Pages 667-676
Molecular Mining of Follicular Fluid for Reliable Biomarkers of Human Oocyte and Embryo Developmental Competence....Pages 677-685
Back Matter....Pages 687-703