Practical Biochemistry for Colleges

Content: 
Titles of Related Interest, Page ii
Front Matter, Page iii
Copyright, Page iv
Preface, Pages xi-xii, E.J. WOOD
Some Properties of an Enzyme: a Demonstration Experiment with Alkaline Phosphatase, Page 3, D F EVERED
A Simple Laboratory Experiment to Demonstrate Transamination, Pages 5-6, SUSAN DEWHURST, IAN SMALLMAN
Simple Visual Demonstrations of the Catalytic Activity of Immobilized Cells and Enzymes, Pages 7-8, PETER S J CHEETHAM, CHRISTOPHER BUCKE
Activation and Inactivation of Sulphydryl and Aspartate Proteases, Pages 9-11, JISNUSON SVASTI
Distribution of Activity of Tyrosinase in the Mushroom, Pages 13-15, M BOIRET, A MARTY, M DEUMIÉ
Demonstration of Carboxylase Enzyme Activity in Pea Pod and Seed Tissues, Pages 17-18, D N PRICE, M E DONKIN
An Experiment in Enzyme Characterization: Banana Polyphenoloxidase, Pages 19-20, MICHAEL C ARCHER, JAMES K PALMER
Purification of Catalase, Pages 21-23, MONTSERRAT BUSQUETS, RAFAEL FRANCO
Purification and Characterization of Escherichia coli Alkaline Phosphatase, Pages 25-27, LIANNA MUNSON, R RAY FALL
Determination of Blanch Efficacy by Means of a Rapid Colorimetric Peroxidase Assay, Pages 29-30, BRUCE P WASSERMAN, JEFFREY D WAGNER
The Peroxidase-Glucose Oxidase Enzyme System, Pages 31-32, ELISA WOOLRIDGE, SANDRA L TURCHI, JOHN R EDWARDS
Tyrosine Amino Transferase, Pages 33-35, MARIE-CHRISTINE RAIN-GUION, HENRIETTE CHAMBON
Purification and Characterization of Firefly Luciferase, Pages 37-39, JUDY A HALL, JoANN J WEBSTER, FRANKLIN R LEACH
Simple Practical Investigations Using Invertase, Pages 41-42, EMMA ASARE-BROWN, CLIVE BULLOCK
Purification of Egg-white Lysozyme by Ion-exchange Chromatography, Pages 43-44, R H C STRANG
Factors Influencing the Rate of an Enzyme Catalyzed Reaction: Lysozyme, Pages 45-47, BARTH H RAGATZ, DIANE K WERTH, JOHN F BONNER JR
Acid Phosphatase as a Teaching Protein, Pages 49-50, FRANCO BONOMI
Inhibition of α-Chymotrypsin by Diethyl p-Nitrophenyl Phosphate, Pages 51-52, R W HANSON
Electrophoresis of Creatine Kinase Isozymes, Pages 53-54, STEVEN H GROSSMAN
Chymotrypsin — a Combined Experiment and Computer Simulation of Pre-steady State and Steady-state Enzyme Kinetics, Pages 55-56, T BRITTAIN
An Integrated Enzyme Kinetics Laboratory Sequence, Pages 57-58, MICHAEL L BUCHOLTZ
Cation-sensitive ATPases, Page 59, J L HOWLAND, A W GARFIELD
Experiments Illustrating Gel Filtration, Pages 63-64, JEAN M WALLACH
Gel Filtration of Haemoglobin, Pages 65-66, H B F DIXON
Introductory practical classes in gel filtration, ion-exchange and thin layer chromatography, Pages 67-68, VERA VERSÉE
A Simple and Colourful Procedure to Demonstrate the Principles of Affinity Chromatography, Pages 69-71, ANTHONY J TURNER
Disc Gel Electrophoresis of Histones and Protamines, Pages 73-74, SALVATORE F RUSSO, RANDALL G ENGEL, MARVIN FRITZ
Polyacrylamide Gel Electrophoresis Using a 12 volt Battery Charger as a Power Pack. High Resolution Microgels on a Low Budget, Pages 75-76, J H WALKER
The Anthocyanin–Betalain Alternative: Electrophoresis, TLC and Chemotaxonomy, Pages 77-78, B P KREMER
Enzymatic Synthesis of Uridine Diphosphate [14C] Glucose, Pages 79-80, JOHN S ANDERSON
Affinity Chromatography and Radioimmunoassay: Purification and Assay of Insulin, Pages 81-83, BONNIE LEE, JEAN-CLAUDE DILLON, GEORGE WOLF
An Inexpensive Oxygen Electrode Apparatus, Pages 85-87, B J GRAY, R HUMPHERSON, E J WOOD
A Tyrosinase Electrode, Pages 89-91, J L IBORRA, E VILANOVA, J A LOZANO
A Simple Scoring Method to Find Membrane-Spanning Peptides, Pages 93-96, LAWRENCE C DAVIS
A Visual-practical Method for Following the Immobilization of Biomolecules, Pages 97-98, A MANJÓN, M J BONETE, F I LLORCA, A JIMENO, J L IBORRA
Glycolysis and Gluconeogenesis, Pages 101-102, R W HANSON
Bottled Glycolysis, Pages 103-104, HUGH A AKERS, JAMES M LONG
Glycolysis Revisited, Pages 105-106, A R JONES
A Study of the Distribution of Flux Control Coefficients in an in vitro Metabolic System, Pages 107-108, N V TORRES, E MELÉNDEZ-HEVIA, J M RIOL-CIMAS
Hormonal Regulation of Gluconeogenesis in Isolated Rat Hepatocytes, Page 109, DAVID A HUME, MAURICE J WEIDEMANN
Appendix: Preparation of Isolated Liver Cells, Page 111, FRANCISCO J ROMERO, JOSÉ VIÑA
Laboratory Experiments on the Regulation of Pyruvate Kinase Isoenzymes, Pages 113-114, E HANNAPPEL, W FISCHER, K BRAND
Investigation of Oxidative Phosphorylation by Mitochondria with the Aid of an Apple IIe Microcomputer, Pages 115-117, R P LEARMONTH
The Stimulation of Protein Kinase by Cyclic-AMP, Page 119, A D LANDMAN
Characterization of the Purine Transport System in Yeast, Pages 121-122, R SCHMIDT, U REICHERT
Amino Acid and Glucose Transport in Small Intestine Everted Sac, Pages 123-124, ZECHARIA MADAR
Diauxie and Determination of Sugars, Pages 125-126, MARGARET ESSENBERG, JUDY A HALL
Catabolite Repression, Catabolite Inhibition and the Regulation of β-Galactosidase Synthesis in Escherichia coli, Pages 127-130, IAN D HAMILTON, CHARLES A FEWSON, W HARRY HOLMES
Selective Cation Transport by Specific Antibiotics, Pages 131-132, BARTH H RAGATZ, GINA MODRAK
Herbicides: Inhibition of the Hill Reaction, Pages 133-135, J W DICKS
Analysis of Chromatin Structure and Composition, Pages 139-141, J E PÉREZ-ORTÍN, E MATALLANA, V TORDERA
Isolation of Biologically Active mRNA, Pages 143-145, STEPHEN SPINDLER, PAUL SIEBERT, FRITZ COFFMAN, FRANCES JURNAK
Preparation of mRNA-dependent Rabbit Reticulocyte Lysate, Pages 147-148, JAMES TOMLINSON, ANN MARIE PENDERGAST, TINA HRONIS, FRANCES JURNAK
Restriction Enzyme Mapping of Bacteriophage Lambda DNA, Pages 149-151, NOREEN CUNNINGHAM, JAMES TOMLINSON, FRANCES JURNAK
Rapid Plasmid Isolation. A Laboratory Experiment for Intermediate and Advanced Students, Pages 153-155, J E PEREZ-ORTIN, D RAMON, S FERRER, V TORDERA
An Introduction to Techniques used in Gene Cloning: Use of Biotin-labelled Probes, Pages 157-159, P M GOODWIN, M UWINS
Cloning and Expression of the Catechol 2,3-oxygenase Gene (xyl E) Using High- and Low-copy Number Vectors, Pages 161-165, JOACHIM FREY, F C H FRANKLIN, JACQUES DESHUSSES
Cloning and Characterization of the PL Promoter of Bacteriophage Lambda, Pages 167-170, RAUL H ANDINO, ALEJANDRA MANDEL, GABRIEL AISEMBERG, HECTOR N TORRES
Estimation of the Asymmetrical Arrangement of Plasma Membrane Aminophospholipids, Pages 171-173, J SANCHEZ-YAGÜE, J A CABEZAS, M LLANILLO
Molecular Models: Paper Formulae and Pop-up Models, Pages 177-179, BERNARD S BROWN, ELIZABETH M EVANS
The Use of Paper Molecular Models as an Aid in Lectures on Oligosaccharide and Polysaccharide Structures, Pages 181-182, M ALEMANY
A Cut-out Model of the α-Helix for Student Use, Pages 183-184, JOSE C CAMESELLE, ANTONIO SILLERO
A Large Polypeptide Chain Model Capable of Realistic Folding, Page 185, ROBERT J YON
A Solomon\'s Knot DNA, Page 187, JOHN FIELDHOUSE
An Inexpensive and Flexible Model-building System, Pages 189-192, JOHN FIELDHOUSE
A Soft Bacteriophage, Page 193, FIONA WILSON
The Sucrose Content of Breakfast Cereals, Page 197, DAN P ROSSON, THOMAS P CHIRPICH
The Determination of Sialic Acid — An Experiment That Demonstrates Many Important Aspects of Spectrophotometric Assays, Pages 199-200, RICHARD O\'KENNEDY
Investigation of Milk Sugar, Pages 201-202, CHRISTOPHER A SMITH, MAUREEN M DAWSON
Determination of Glycosylated Hemoglobin, Pages 203-204, M H KOENST, R D EDSTROM
Demonstration of the Specificity of an Enzymatic Method for Plasma Paracetamol Estimation, Pages 205-206, JOHN A O\'MULLANE
Determining Blood Enzyme Concentrations: a Unified Laboratory Experiment, Pages 207-208, JACK E DIXON
Acetylator Phenotyping and Genotyping of Students Using Sulphadimidine, Pages 209-210, A H BITTLES
Physiological Interactions of Bilirubin, Pages 211-213, M SHANLEY, J NAGYVARY
Platelet Aggregation in Whole Blood: A Laboratory Experiment for a Medical Biochemistry Course, Pages 215-216, JÜRGEN LÜTHJE, ADALING OGILVIE
Estimation of Amniotic Fluid Phospholipids in Assessing Fetal Lung Maturity, Pages 217-219, BARTH H RAGATZ, GINA MODRAK
Glucocorticoid Receptors: A Glass Fiber Filter Assay to Determine Steroid Specificity and Equilibrium Binding Properties, Pages 221-223, NANCY J JOHN, GARY L FIRESTONE
Index, Page 225