Potassium Channels: Methods and Protocols

Front Matter ....Pages i-xvi
 Manipulating Potassium Channel Expression and Function in Hippocampal Neurons by In Utero Electroporation  (Kang Wang)....Pages 1-5
 Studying KCNQ1 Mutation and Drug Response in Type 1 Long QT Syndrome Using Patient-Specific Induced Pluripotent Stem Cell-Derived Cardiomyocytes  (Heming Wei, Jianjun Wu, Zhenfeng Liu)....Pages 7-28
 Monitoring Changes in the Abundance of Endogenously Expressed ATP-Sensitive Potassium (KATP) Channels in the Plasma Membrane Using Surface Biotinylation  (Jing-Syuna Ruan, Pei-Chun Chen)....Pages 29-36
 Nonsense-Mediated mRNA Decay of hERG Mutations in Long QT Syndrome  (Qiuming Gong, Zhengfeng Zhou)....Pages 37-49
 Probing Subunits Interactions in KATP Channels Using Photo-Crosslinking via Genetically Encoded p-Azido-l-phenylalanine  (Prasanna Devaraneni, Emily A. Rex, Show-Ling Shyng)....Pages 51-61
 Hyper-SUMOylation of K+ Channels in Sudden Unexplained Death in Epilepsy: Isolation and Primary Culture of Dissociated Hippocampal Neurons from Newborn Mice for Subcellular Localization  (Xu Chen, Shanshan Zhang, Jifang Huang, Wanying Dong, Hui Xiao, Huanjie Shao et al.)....Pages 63-71
 Simultaneous Real-Time Measurement of the β-Cell Membrane Potential and Ca2+ Influx to Assess the Role of Potassium Channels on β-Cell Function  (Nicholas C. Vierra, Matthew T. Dickerson, Louis H. Philipson, David A. Jacobson)....Pages 73-84
 Methods for Characterizing Disease-Associated ATP-Sensitive Potassium Channel Mutations  (Balamurugan Kandasamy, Show-Ling Shyng)....Pages 85-104
 Thallium Flux Assay for Measuring the Activity of Monovalent Cation Channels and Transporters  (C. David Weaver)....Pages 105-114
 Nuclear Magnetic Resonance Approaches for Characterizing Protein-Protein Interactions  (Yuki Toyama, Yoko Mase, Hanaho Kano, Mariko Yokogawa, Masanori Osawa, Ichio Shimada)....Pages 115-128
 Studying Mechanosensitivity of Two-Pore Domain K+ Channels in Cellular and Reconstituted Proteoliposome Membranes  (Josefina del Mármol, Robert A. Rietmeijer, Stephen G. Brohawn)....Pages 129-150
 Migration of PIP2 on KCNQ2 Surface Revealed by Molecular Dynamics Simulations  (Qiansen Zhang, Huaiyu Yang)....Pages 151-161
 Studying Structural Dynamics of Potassium Channels by Single-Molecule FRET  (Shizhen Wang, Joshua B. Brettmann, Colin G. Nichols)....Pages 163-180
 Patch-Clamp Recordings of the KcsA K+ Channel in Unilamellar Blisters  (Kimberly Matulef, Francis I. Valiyaveetil)....Pages 181-191
 Combinatorial Assembly of Lumitoxins  (David Nedrud, Daniel Schmidt)....Pages 193-209
 Characterization of MC4R Regulation of the Kir7.1 Channel Using the Tl+ Flux Assay  (Michael J. Litt, Roger D. Cone, Masoud Ghamari-Langroudi)....Pages 211-222
 Stopped-Flow Fluorometric Ion Flux Assay for Ligand-Gated Ion Channel Studies  (David J. Posson, Radda Rusinova, Olaf S. Andersen, Crina M. Nimigean)....Pages 223-235
 In Vivo Analysis of Potassium Channelopathies: Loose Patch Recording of Purkinje Cell Firing in Living, Awake Zebrafish  (Jui-Yi Hsieh, Diane M. Papazian)....Pages 237-252
 Site-Directed Unnatural Amino Acid Mutagenesis to Investigate Potassium Channel Pharmacology in Xenopus laevis Oocytes  (Robin Y. Kim, Harley T. Kurata)....Pages 253-263
 Random Spherically Constrained Single-Particle (RSC) Method to Study Voltage-Gated Ion Channels  (Liguo Wang)....Pages 265-277
 CW-EPR Spectroscopy and Site-Directed Spin Labeling to Study the Structural Dynamics of Ion Channels  (Cholpon Tilegenova, Benjamin W. Elberson, D. Marien Cortes, Luis G. Cuello)....Pages 279-288
 Ion Binding to Transport Proteins using Isothermal Titration Calorimetry  (Shian Liu, Steve W. Lockless)....Pages 289-303
 Building Atomic Models of the Ion Channels Based on Low Resolution Electron Microscopy Maps and Homology Modeling  (Valery Novoseletsky, Olfat A. Malak, Gildas Loussouarn, Olga S. Sokolova)....Pages 305-319
 Studying Kv Channels Function using Computational Methods  (Audrey Deyawe, Marina A. Kasimova, Lucie Delemotte, Gildas Loussouarn, Mounir Tarek)....Pages 321-341
Back Matter ....Pages 343-345