Polyamines: Methods and Protocols

Front Matter ....Pages i-xv
 Polyamines in the Context of Metabolic Networks  (Wegi Wuddineh, Rakesh Minocha, Subhash C. Minocha)....Pages 1-23
 Molecules for Sensing Polyamines and Transducing Their Action in Plants  (Tomonobu Kusano, G. H. M. Sagor, Thomas Berberich)....Pages 25-35
 Polyamine Metabolism Responses to Biotic and Abiotic Stress  (Fernando M. Romero, Santiago J. Maiale, Franco R. Rossi, Maria Marina, Oscar A. Ruíz, Andrés Gárriz)....Pages 37-49
 Thermospermine: An Evolutionarily Ancient but Functionally New Compound in Plants  (Taku Takahashi)....Pages 51-59
 Determination of Polyamines by Capillary Electrophoresis Using Salicylaldehyde-5-Sulfonate as a Derivatizing Reagent  (Takashi Kaneta)....Pages 61-68
 Detection of Thermospermine and Spermine by HPLC in Plants  (Taku Takahashi, Ayaka Takano, Jun-Ichi Kakehi)....Pages 69-73
 Development of Amine-Oxidase-Based Biosensors for Spermine and Spermidine Analysis  (Cristina Tortolini, Gabriele Favero, Franco Mazzei)....Pages 75-80
 Identification of Branched-Chain Polyamines in Hyperthermophiles  (Ryota Hidese, Wakao Fukuda, Masaru Niitsu, Shinsuke Fujiwara)....Pages 81-94
 Analysis of Polyamines Conjugated with Hydroxycinnamoyl Acids by High-Performance Liquid Chromatography Coupled to Electrospray Ionization Tandem Mass Spectrometry  (Laura Torras-Claveria, Jaume Bastida, Francesc Viladomat, Antonio F. Tiburcio)....Pages 95-104
 Analysis of Glutathione in Biological Samples by HPLC Involving Pre-Column Derivatization with o-Phthalaldehyde  (Yongqing Hou, Xilong Li, Zhaolai Dai, Zhenlong Wu, Fuller W. Bazer, Guoyao Wu)....Pages 105-115
 Determination of Arginine and Ornithine Decarboxylase Activities in Plants  (Rubén Alcázar, Antonio F. Tiburcio)....Pages 117-122
 Determination of S-Adenosylmethionine Decarboxylase Activity in Plants  (Antonio F. Tiburcio, Rubén Alcázar)....Pages 123-128
 Determination of Copper Amine Oxidase Activity in Plant Tissues  (Riccardo Angelini, Alessandra Cona, Paraskevi Tavladoraki)....Pages 129-139
 Determination of di−/Polyamine Oxidase Activity in Plants by an In-Gel Spermidine Oxidation Assay  (Panagiotis N. Moschou)....Pages 141-147
 Pentamine as a Substrate for Measuring Spermine Oxidase Activity  (Koichi Takao, Yoshiaki Sugita)....Pages 149-154
 Spectrophotometric Quantification of Reactive Oxygen, Nitrogen and Sulfur Species in Plant Samples  (Chrystalla Antoniou, Andreas Savvides, Egli C. Georgiadou, Vasileios Fotopoulos)....Pages 155-161
 Novel Route for Agmatine Catabolism in Aspergillus niger: 4-Guanidinobutyrase Assay  (Tejaswani Saragadam, Narayan S. Punekar)....Pages 163-172
 Determination of Transglutaminase Activity in Plants  (S. Del Duca, P. L. R. Bonner, I. Aloisi, D. Serafini-Fracassini, G. Cai)....Pages 173-200
 Procedures for ADC Immunoblotting and Immunolocalization for Transmission Electron Microscopy During Organogenic Nodule Formation in Hop  (Ana Margarida Fortes, Jose M. Seguí-Simarro)....Pages 201-214
 Analysis of the Intracellular Localization of Transiently Expressed and Fluorescently Labeled Copper-Containing Amine Oxidases, Diamine Oxidase and N-Methylputrescine Oxidase in Tobacco, Using an Agrobacterium Infiltration Protocol  (Tsubasa Shoji)....Pages 215-223
 Techniques Used for Functional Characterization of Polyamine Transporters  (Claudio A. Pereira, Melisa Sayé, Chantal Reigada, Mariana R. Miranda)....Pages 225-232
 Quantitative Trait Loci for Root Growth Response to Cadaverine in Arabidopsis  (Nicole M. Gibbs, Laura Vaughn Rouhana, Patrick H. Masson)....Pages 233-256
 Methods Related to Polyamine Control of Cation Transport Across Plant Membranes  (Isaac Zepeda-Jazo, Igor Pottosin)....Pages 257-276
 Analysis of DNA Methylation Content and Patterns in Plants  (Andreas Finke, Wilfried Rozhon, Ales Pecinka)....Pages 277-298
 Investigating Ornithine Decarboxylase Posttranscriptional Regulation Via a Pulldown Assay Using Biotinylated Transcripts  (Anh Mai, Shannon L. Nowotarski)....Pages 299-308
 Analysis of Cotranslational Polyamine Sensing During Decoding of ODC Antizyme mRNA  (R. Palanimurugan, Daniela Gödderz, Leo Kurian, R. Jürgen Dohmen)....Pages 309-323
 Modulation of Protein Synthesis by Polyamines in Mammalian Cells  (Keiko Kashiwagi, Yusuke Terui, Kazuei Igarashi)....Pages 325-336
 Determination of Posttranslational Modifications by 2D PAGE: Applications to Polyamines  (Marta Bitrián, Antonio F. Tiburcio, Rubén Alcázar)....Pages 337-341
 Generation of EMS-Mutagenized Populations of Arabidopsis thaliana for Polyamine Genetics  (Kostadin E. Atanasov, Changxin Liu, Antonio F. Tiburcio, Rubén Alcázar)....Pages 343-346
 Transcriptome Analysis of PA Gain and Loss of Function Mutants  (Francisco Marco, Pedro Carrasco)....Pages 347-371
 High-Throughput Phenotyping in Plant Stress Response: Methods and Potential Applications to Polyamine Field  (D. Marko, N. Briglia, S. Summerer, A. Petrozza, F. Cellini, R. Iannacone)....Pages 373-388
 Abiotic Stress Phenotyping of Polyamine Mutants  (Thomas Berberich, G. H. M. Sagor, Tomonobu Kusano)....Pages 389-403
 Phenotypic and Genotypic Characterization of Mutant Plants in Polyamine Metabolism Genes During Pathogenic Interactions  (Franco R. Rossi, Fernando M. Romero, Oscar A. Ruíz, Maria Marina, Andrés Gárriz)....Pages 405-416
 Real-Time In Vivo Monitoring of Reactive Oxygen Species in Guard Cells  (Ky Young Park, Kalliopi A. Roubelakis-Angelakis)....Pages 417-425
 Genome-Wide Association Mapping Analyses Applied to Polyamines  (Luis Barboza-Barquero, Paul Esker, Rubén Alcázar)....Pages 427-432
 Polyamine Metabolism in Climacteric and Non-Climacteric Fruit Ripening  (Ana Margarida Fortes, Patricia Agudelo-Romero)....Pages 433-447
 Application of Polyamines to Maintain Functional Properties in Stored Fruits  (María Serrano, Daniel Valero)....Pages 449-458
 Acrolein: An Effective Biomarker for Tissue Damage Produced from Polyamines  (Kazuei Igarashi, Takeshi Uemura, Keiko Kashiwagi)....Pages 459-468
 Polyamines and Cancer  (Elisabetta Damiani, Heather M. Wallace)....Pages 469-488
 Potential Applications of Polyamines in Agriculture and Plant Biotechnology  (Antonio F. Tiburcio, Rubén Alcázar)....Pages 489-508
Back Matter ....Pages 509-512