Plant respiration and internal oxygen : methods and protocols

Front Matter ....Pages i-xiii
 Assessing Metabolic Flux in Plants with Radiorespirometry  (Nicholas J. Kruger, Shyam K. Masakapalli, R. George Ratcliffe)....Pages 1-16
 Coupling Radiotracer Experiments with Chemical Fractionation for the Estimation of Respiratory Fluxes  (Toshihiro Obata, Laise Rosado-Souza, Alisdair R. Fernie)....Pages 17-30
 A Method for Imaging Oxygen Distribution and Respiration at a Microscopic Level of Resolution  (Hardy Rolletschek, Gregor Liebsch)....Pages 31-38
 VisiSens Technique to Measure Internal Oxygen and Respiration in Barley Roots  (Aprajita Kumari, Kapuganti Jagadis Gupta)....Pages 39-45
 MultiSense: A Multimodal Sensor Tool Enabling the High-Throughput Analysis of Respiration  (Peter Keil, Gregor Liebsch, Ljudmilla Borisjuk, Hardy Rolletschek)....Pages 47-56
 Measurement of Respiration and Internal Oxygen in Germinating Cicer arietinum L. Seeds Using Optic Microsensor  (Sonika Pandey, Aprajita Kumari, Chellapilla Bharadwaj, Kapuganti Jagadis Gupta)....Pages 57-62
 Using an Oxygen Microsensor to Measure Oxygen Dynamics in Tomato Plants in Response to Pseudomonas syringae Infection  (Pradeep Kumar Pathak, Kapuganti Jagadis Gupta)....Pages 63-69
 Measurement of Oxygen Status in Arabidopsis Leaves Undergoing the Hypersensitive Response During Pseudomonas Infection  (Aprajita Kumari, Gail M. Preston, Kapuganti Jagadis Gupta)....Pages 71-76
 Isolation of Physiologically Active and Intact Mitochondria from Chickpea  (Sonika Pandey, Aprajita Kumari, Kapuganti Jagadis Gupta)....Pages 77-85
 Isolation and Structural Studies of Mitochondria from Pea Roots  (Abhaypratap Vishwakarma, Kapuganti Jagadis Gupta)....Pages 87-95
 Mitochondrial Respiration and Oxygen Tension  (Daniel S. Shaw, Karlia Meitha, Michael J. Considine, Christine H. Foyer)....Pages 97-113
 Isolation of Mitochondria from Model and Crop Plants  (Sandra M. Kerbler, Nicolas L. Taylor)....Pages 115-142
 Procedures of Mitochondria Purification and Gene Expression to Study Alternative Respiratory and Uncoupling Pathways in Fruits  (Jurandi Gonçalves de Oliveira, Luis Miguel Mazorra Morales, Gláucia Michelle Cosme Silva, Kátia Daniella da Cruz Saraiva, Diederson Bortolino Santana, Clesivan Pereira dos Santos et al.)....Pages 143-165
 Measurement of Tricarboxylic Acid Cycle Enzyme Activities in Plants  (Rebeca Patricia Omena-Garcia, Wagner L. Araújo, Yves Gibon, Alisdair R. Fernie, Adriano Nunes-Nesi)....Pages 167-182
 Respiration Traits as Novel Markers for Plant Robustness Under the Threat of Climate Change: A Protocol for Validation  (Birgit Arnholdt-Schmitt)....Pages 183-191
 Calorespirometry: A Novel Tool in Functional Hologenomics to Select “Green” Holobionts for Biomass Production  (Birgit Arnholdt-Schmitt, Vinod Kumar Patil)....Pages 193-201
 Measurements of Electron Partitioning Between Cytochrome and Alternative Oxidase Pathways in Plant Tissues  (Nestor Fernandez Del-Saz, Miquel Ribas-Carbo, Gabriel Martorell, Alisdair R. Fernie, Igor Florez-Sarasa)....Pages 203-217
 A Driving Bioinformatics Approach to Explore Co-regulation of AOX Gene Family Members During Growth and Development  (José Hélio Costa, Birgit Arnholdt-Schmitt)....Pages 219-224
 A Step-by-Step Protocol for Classifying AOX Proteins in Flowering Plants  (José Hélio Costa, Clesivan Pereira dos Santos, Kátia Daniella da Cruz Saraiva, Birgit Arnholdt-Schmitt)....Pages 225-234
 Studying Individual Plant AOX Gene Functionality in Early Growth Regulation: A New Approach  (Birgit Arnholdt-Schmitt, Vinod Kumar Patil)....Pages 235-244
 Laser Capture Microdissection for Amplification of Alternative Oxidase (AOX) Genes in Target Tissues in Daucus carota L.  (Carla Ragonezi, Birgit Arnholdt-Schmitt)....Pages 245-252
 Measurement of Mitochondrial Respiration in Isolated Protoplasts: Cytochrome and Alternative Pathways  (Bobba Sunil, Agepati S. Raghavendra)....Pages 253-265
 Measuring Spatial and Temporal Oxygen Flux Near Plant Tissues Using a Self-Referencing Optrode  (Eric S. McLamore, D. Marshall Porterfield, Yinglang Wan)....Pages 267-281
Back Matter ....Pages 283-285