Plant Protoplasts and Genetic Engineering II

Front Matter....Pages I-XX
Genetic Engineering and In Vitro Manipulation of Plant Cells — Technical Advances....Pages 1-25
Genetic Manipulation: Generative Versus Somatic....Pages 26-53
Uptake and Integration of Exogenous DNA in Plants....Pages 54-74
DNA Recombinants and Transformation of Agricultural Crops....Pages 75-98
Genetically Transformed Maize ( Zea mays L.) Plants from Protoplasts....Pages 99-106
Protoplast Culture and Transformation Studies on Rice....Pages 107-121
Transformation in Potato....Pages 122-139
Transformation in Cotton....Pages 140-154
Transformation in Populus spp.....Pages 155-172
Transformation in Vinca rosea L.: An Example of Spheroplast Method for Gene Transfer....Pages 173-187
Transformation in Hyoscyamus ....Pages 188-196
Genetic Manipulation in Brassica ....Pages 197-216
Direct Gene Transfer in Protoplasts of Nicotiana plumbaginifolia ....Pages 217-227
Microinjection of Cells and Protoplasts: Integration of Foreign DNA....Pages 228-240
Electrofusion: The Technique and Its Application to Somatic Hybridization....Pages 241-256
Versatile Apparatuses for Electrogene Mapping, Electrophoresis and Electrofusion....Pages 257-282
Chloroplast Genomes as Genetic Markers....Pages 283-303
Flow Cytometric Analysis and Sorting of Somatic Hybrid and Transformed Protoplasts....Pages 304-327
Isolation of Nuclei and Their Transplantation into Plant Protoplasts....Pages 328-342
Chromosome Transplantation and Applications of Flow Cytometry in Plants....Pages 343-359
Transfer of Mitochondria Through Protoplast Fusion....Pages 360-375
Incorporation of the Firefly Luciferase Gene into Plant Cells....Pages 376-387
Uptake of Viruses by Plant Protoplasts and Their Use as Transforming Agents....Pages 388-405
Uptake of Fungal Protoplasts by Plant Protoplasts....Pages 406-427
Chloroplast Uptake and Genetic Complementation....Pages 428-446
Cytoplasts: Isolation and Uses....Pages 447-458
Vacuoles: Isolation, Purification, and Uses....Pages 459-479
Miniprotoplasts and Their Use in Genetic Engineering....Pages 480-493
Back Matter....Pages 495-499