Plant Cold Acclimation: Methods and Protocols

Front Matter ....Pages i-xii
 Introduction: Plant Cold Acclimation and Winter Survival  (Dirk K. Hincha, Ellen Zuther)....Pages 1-7
 Measuring Freezing Tolerance of Leaves and Rosettes: Electrolyte Leakage and Chlorophyll Fluorescence Assays  (Anja Thalhammer, Majken Pagter, Dirk K. Hincha, Ellen Zuther)....Pages 9-21
 Differential Thermal Analysis: A Fast Alternative to Frost Tolerance Measurements  (Andrey V. Malyshev, Ilka Beil, Juergen Kreyling)....Pages 23-31
 Infrared Thermal Analysis of Plant Freezing Processes  (Gilbert Neuner, Edith Lichtenberger)....Pages 33-41
 Conducting Field Trials for Frost ToleranceBreeding in Cereals  (Luigi Cattivelli, Cristina Crosatti)....Pages 43-52
 A Whole-Plant Screening Test to Select Freezing-Tolerant and Low-Dormant Genotypes  (Annick Bertrand, Annie Claessens, Josée Bourassa, Solen Rocher, Vern S. Baron)....Pages 53-60
 Mapping of Quantitative Trait Loci (QTL) Associated with Plant Freezing Tolerance and Cold Acclimation  (Evelyne Téoulé, Carine Géry)....Pages 61-84
 Identification of Arabidopsis Mutants with Altered Freezing Tolerance  (Carlos Perea-Resa, Rafael Catalá, Julio Salinas)....Pages 85-97
 Cryo-Scanning Electron Microscopy to Study the Freezing Behavior of Plant Tissues  (Seizo Fujikawa, Keita Endoh)....Pages 99-117
 Using Pixel-Based Microscope Images to Generate 3D Reconstructions of Frozen and Thawed Plant Tissue  (David P. Livingston III, Tan D. Tuong)....Pages 119-139
 Phenotyping Plant Cellular and Tissue Level Responses to Cold with Synchrotron-Based Fourier-Transform Infrared Spectroscopy and X-Ray Computed Tomography  (Ian R. Willick, Jarvis Stobbs, Chithra Karunakaran, Karen K. Tanino)....Pages 141-159
 Proteomic Approaches to Identify Proteins Responsive to Cold Stress  (Anna M. Jozefowicz, Stefanie Döll, Hans-Peter Mock)....Pages 161-170
 Proteomic Approaches to Identify Cold-Regulated Plasma Membrane Proteins  (Md Mostafa Kamal, Daisuke Takahashi, Takato Nakayama, Yushi Miki, Yukio Kawamura, Matsuo Uemura)....Pages 171-186
 A Lipidomic Approach to Identify Cold-Induced Changes in Arabidopsis Membrane Lipid Composition  (Yu Song, Hieu Sy Vu, Sunitha Shiva, Carl Fruehan, Mary R. Roth, Pamela Tamura et al.)....Pages 187-202
 Multiplexed Profiling and Data Processing Methods to Identify Temperature-Regulated Primary Metabolites Using Gas Chromatography Coupled to Mass Spectrometry  (Alexander Erban, Federico Martinez-Seidel, Yogeswari Rajarathinam, Frederik Dethloff, Isabel Orf, Ines Fehrle et al.)....Pages 203-239
 Determining the ROS and the Antioxidant Status of Leaves During Cold Acclimation  (Andras Bittner, Thomas Griebel, Jörn van Buer, Ilona Juszczak-Debosz, Margarete Baier)....Pages 241-254
 Analysis of Changes in Plant Cell Wall Composition and Structure During Cold Acclimation  (Daisuke Takahashi, Ellen Zuther, Dirk K. Hincha)....Pages 255-268
 Subcellular Compartmentation of Metabolites Involved in Cold Acclimation  (Imke I. Hoermiller, Thomas Nägele, Arnd G. Heyer)....Pages 269-275
 Mathematical Modeling of Plant Metabolism in a Changing Temperature Regime  (Lisa Fürtauer, Thomas Nägele)....Pages 277-287
 Characterization of Ice-Binding Proteins from Sea-Ice Microalgae  (Maddalena Bayer-Giraldi, EonSeon Jin, Peter W. Wilson)....Pages 289-302
 Isolation and Characterization of Ice-Binding Proteins from Higher Plants  (Melissa Bredow, Heather E. Tomalty, Laurie A. Graham, Audrey K. Gruneberg, Adam J. Middleton, Barbara Vanderbeld et al.)....Pages 303-332
Back Matter ....Pages 333-336