Physical Methods on Biological Membranes and Their Model Systems

Front Matter....Pages i-ix
Physical Methods on Biological Membranes and Their Model Systems: A Perspective....Pages 1-3
High Resolution NMR Spectroscopy in Liquids and Solids....Pages 5-25
Basic Principles of Deuterium and Phosphorus-31-Magnetic Resonance....Pages 27-37
Use of Electron Spin Resonance to Study Complex Biological Membranes....Pages 39-53
Freeze-Etching Electron Microscopy: Recent Developments and Application to the Study of Biological Membranes and Their Components....Pages 55-71
The Nature of Intramembraneous Particles....Pages 73-80
Lateral and Polymorphic Phase Transitions in Relation to the Barrier Function of a Lipid Membrane....Pages 81-93
Fluorescence Energy Transfer as a Structural Probe in Membranes and Membrane-Bound Proteins....Pages 95-122
The Interaction of Intrinsic Proteins and Lipids in Biomembranes....Pages 123-133
Conformational Studies of Membrane Proteins by High-Resolution NMR....Pages 135-140
Water Organization in Reversed Micelles....Pages 141-162
Lipid-Protein Interactions: From Crystalline Lipoproteins to Intact Membranes....Pages 163-177
Membrane Fusion and Lipid Polymorphism....Pages 179-192
Control of Membrane Fusion by Divalent Cations, Phospholipid Head-Groups and Proteins....Pages 193-218
Correlation between Steady-State and Time-Resolved Fluorescence Anisotropy Data....Pages 219-226
Membrane Lipid Fluidity at a Physiologically Relevant Scale; Determination by Fluorescence Polarization....Pages 227-243
Dynamic Interactions of Fluorescently Labeled Adenosine Deaminase in Intact Human Erythrocytes: Relation to Abnormal Activity in Malignant Cells....Pages 245-250
Phase Separation in Phospholipid Mixtures by Fluorescence Anisotropy....Pages 251-260
Membrane Fluidity and Lipid Composition....Pages 261-276
Membrane Fluidity and Membrane Activities....Pages 277-290
Membrane Fluorescence Anisotropy Behavior during Cell Cycle....Pages 291-296
Stable Biomembrane Surfaces Formed by Phospholipid Polymers....Pages 297-303
Utilizing Mast Cells and Basophils to Study Lipoxygenase Involvement in Membrane Activation....Pages 305-343
Immunoglobulin Surface Adsorption Studied by Total Internal Reflection with Fluorescence Correlation Spectroscopy....Pages 345-349
Enthalpy State of Different Liposome Preparations of Dimyristoyl Phosphatidylcholine and Their Stability in the Presence of a Complex Forming Protein....Pages 351-355
Free-Flow Electrophoresis as a Suitable Tool to Explore Intervesicular Interactions....Pages 357-361
Solid State 19 F NMR in ‘Model’ Membrane Systems....Pages 363-367
Organized Cultures of Nerve Tissue: A Novel Model System for Studies of Lipid Protein Interaction on the Functional Level....Pages 369-395
Partition of Proteins Between Water and Non-Polar Phases....Pages 397-406
Photomodification of Biomembranes....Pages 407-450
Back Matter....Pages 451-459