Peptidomics: Methods and Strategies

Front Matter ....Pages i-xvii
Front Matter ....Pages 1-1
 Origins, Technological Development, and Applications of Peptidomics  (Michael Schrader)....Pages 3-39
 Brain Tissue Sample Stabilization and Extraction Strategies for Neuropeptidomics  (Elva Fridjonsdottir, Anna Nilsson, Henrik Wadensten, Per E. Andrén)....Pages 41-49
 Isolation of Endogenous Peptides from Cultured Cell Conditioned Media for Mass Spectrometry  (Kazuki Sasaki, Takashi Tsuchiya, Tsukasa Osaki)....Pages 51-58
 Mass Spectrometric Identification of Endogenous Peptides  (Mikel Azkargorta, Iraide Escobes, Ibon Iloro, Felix Elortza)....Pages 59-70
 Bioinformatics for Prohormone and Neuropeptide Discovery  (Bruce R. Southey, Elena V. Romanova, Sandra L. Rodriguez-Zas, Jonathan V. Sweedler)....Pages 71-96
 Substrate Capture Assay Using Inactive Oligopeptidases to Identify Novel Peptides  (Vanessa Rioli, Emer S. Ferro)....Pages 97-105
 Non-targeted Identification of d-Amino Acid-Containing Peptides Through Enzymatic Screening, Chiral Amino Acid Analysis, and LC-MS  (Hua-Chia Tai, James W. Checco, Jonathan V. Sweedler)....Pages 107-118
Front Matter ....Pages 119-119
 Quantitative Peptidomics: General Considerations  (Lloyd Fricker)....Pages 121-140
 Quantitative Peptidomics with Isotopic and Isobaric Tags  (Kurt Boonen, Wouter De Haes, Joris Van Houtven, Rik Verdonck, Geert Baggerman, Dirk Valkenborg et al.)....Pages 141-159
 Quantitative Peptidomics Using Reductive Methylation of Amines  (Sayani Dasgupta, Leandro M. Castro, Alexandre K. Tashima, Lloyd Fricker)....Pages 161-174
 Metabolic Labeling to Quantify Drosophila Neuropeptides and Peptide Hormones  (Thomas Otto Kunz, Jiangtian Chen, Megha, Christian Wegener)....Pages 175-185
 Data Preprocessing, Visualization, and Statistical Analyses of Nontargeted Peptidomics Data from MALDI-MS  (Harald Tammen, Rüdiger Hess)....Pages 187-196
Front Matter ....Pages 197-197
 Affinity Purification of Neuropeptide Precursors from Mice Lacking Carboxypeptidase E Activity  (Lloyd Fricker)....Pages 199-208
 Mass Spectrometry Based Immunopeptidomics for the Discovery of Cancer Neoantigens  (Michal Bassani-Sternberg)....Pages 209-221
 Milk Peptidomics to Identify Functional Peptides and for Quality Control of Dairy Products  (David Dallas, Søren Drud Nielsen)....Pages 223-240
 Neuropeptidomic Analysis of Zebrafish Brain  (Kristien A. Van Camp, Geert Baggerman, Ronny Blust, Steven J. Husson)....Pages 241-246
 Identification, Quantitation, and Imaging of the Crustacean Peptidome  (Kellen DeLaney, Amanda Buchberger, Lingjun Li)....Pages 247-269
 Identification of Endogenous Neuropeptides in the Nematode C. elegans Using Mass Spectrometry  (Sven Van Bael, Samantha L. Edwards, Steven J. Husson, Liesbet Temmerman)....Pages 271-291
 EndoProteoFASP as a Tool to Unveil the Peptidome-Protease Profile: Application to Salivary Diagnostics  (Fábio Trindade, Inês Falcão-Pires, Adelino Leite-Moreira, Pedro S. Gomes, Julie Klein, Rita Ferreira et al.)....Pages 293-310
 Methodology for Urine Peptidome Analysis Based on Nano-HPLC Coupled to Fourier Transform Ion Cyclotron Resonance Mass Spectrometry  (Alexey S. Kononikhin, Victoria A. Sergeeva, Anna E. Bugrova, Maria I. Indeykina, Natalia L. Starodubtseva, Vitaliy V. Chagovets et al.)....Pages 311-318
 Identification of Components in Frog Skin Secretions with Therapeutic Potential as Antidiabetic Agents  (J. Michael Conlon, R. Charlotte Moffett, Jérôme Leprince, Peter R. Flatt)....Pages 319-333
 High-Accuracy Mass Spectrometry Based Screening Method for the Discovery of Cysteine Containing Peptides in Animal Venoms and Toxins  (Luuk N. van Oosten, Martijn W. H. Pinkse, Mervin Pieterse, Pierre Escoubas, Peter D. E. M. Verhaert)....Pages 335-348
 Analysis of the Snake Venom Peptidome  (Solange M. T. Serrano, André Zelanis, Eduardo S. Kitano, Alexandre K. Tashima)....Pages 349-358
 Identification of Peptides in Spider Venom Using Mass Spectrometry  (Rafael L. Lomazi, Erika S. Nishiduka, Pedro I. Silva Jr, Alexandre K. Tashima)....Pages 359-367
 Single Cell Peptidomics: Approach for Peptide Identification by N-Terminal Peptide Derivatization  (Susanne Neupert)....Pages 369-378
 Peptidomic Identification of Cysteine-Rich Peptides from Plants  (Xinya Hemu, Aida Serra, Dina A. Darwis, Tobias Cornvik, Siu Kwan Sze, James P. Tam)....Pages 379-393
 Analysis of Endogenous Peptide Pools of Physcomitrella patens Moss  (Igor Fesenko, Regina Khazigaleeva, Vadim Govorun, Vadim Ivanov)....Pages 395-405
 The Bright Future of Peptidomics  (Peter D. E. M. Verhaert)....Pages 407-416
Back Matter ....Pages 417-423