Part B: Proteolytic Enzymes

Content: 
Series Editors
Page ii

Copyright page
Page iv

Contributors to Volume 452
Pages xiii-xIX

Preface
Pages xxi-xxii
Daniel J. Klionsky

Volumes in Series
Pages xxiii-xlix

Chapter 1 Monitoring Autophagy in Mammalian Cultured Cells through the Dynamics of LC3 Review Article
Pages 1-12
Shunsuke Kimura, Naonobu Fujita, Takeshi Noda, Tamotsu Yoshimori

Chapter 2 Methods for Monitoring Autophagy Using GFP‐LC3 Transgenic Mice Review Article
Pages 13-23
Noboru Mizushima

Chapter 3 Using Photoactivatable Proteins to Monitor Autophagosome Lifetime Review Article
Pages 25-45
Dale W. Hailey, Jennifer Lippincott‐Schwartz

Chapter 4 Assaying of Autophagic Protein Degradation Review Article
Pages 47-61
Chantal Bauvy, Alfred J. Meijer, Patrice Codogno

Chapter 5 Sequestration Assays for Mammalian Autophagy Review Article
Pages 63-83
Per O. Seglen, Anders Øverbye, Frank Sætre

Chapter 6 Assays to Assess Autophagy Induction and Fusion of Autophagic Vacuoles with a Degradative Compartment, Using Monodansylcadaverine (MDC) and DQ‐BSA Review Article
Pages 85-95
Cristina Lourdes VГЎzquez, MarГ­a Isabel Colombo

Chapter 7 The GST‐BHMT Assay and Related Assays for Autophagy Review Article
Pages 97-118
Patrick B. Dennis, Carol A. Mercer

Chapter 8 Monitoring Starvation‐Induced Reactive Oxygen Species Formation Review Article
Pages 119-130
Ruth Scherz‐Shouval, Zvulun Elazar

Chapter 9 Flow Cytometric Analysis of Autophagy in Living Mammalian Cells Review Article
Pages 131-141
Elena Shvets, Zvulun Elazar

Chapter 10 Monitoring Autophagy by Electron Microscopy in Mammalian Cells Review Article
Pages 143-164
Päivi Ylä‐Anttila, Helena Vihinen, Eija Jokitalo, Eeva‐Liisa Eskelinen

Chapter 11 Monitoring Mammalian Target of Rapamycin (mTOR) Activity Review Article
Pages 165-180
Tsuneo Ikenoue, Sungki Hong, Ken Inoki

Chapter 12 Monitoring Autophagic Degradation of p62/SQSTM1 Review Article
Pages 181-197
Geir Bjørkøy, Trond Lamark, Serhiy Pankiv, Aud Øvervatn, Andreas Brech, Terje Johansen

Chapter 13 Cytosolic LC3 Ratio as a Quantitative Index of Macroautophagy Review Article
Pages 199-213
Motoni Kadowaki, Md. Razaul Karim

Chapter 14 Method for Monitoring Pexophagy in Mammalian Cells Review Article
Pages 215-226
Junji Ezaki, Masaaki Komatsu, Sadaki Yokota, Takashi Ueno, Eiki Kominami

Chapter 15 Mitophagy in Mammalian Cells: The Reticulocyte Model Review Article
Pages 227-245
Ji Zhang, Mondira Kundu, Paul A. Ney

Chapter 16 Assessing Mammalian Autophagy by WIPI‐1/Atg18 Puncta Formation Review Article
Pages 247-260
Tassula Proikas‐Cezanne, Simon G. Pfisterer

Chapter 17 Correlative Light and Electron Microscopy Review Article
Pages 261-275
Minoo Razi, Sharon A. Tooze

Chapter 18 Semiconductor Nanocrystals in Autophagy Research: Methodology Improvement at Nanosized Scale Review Article
Pages 277-296
Oleksandr Seleverstov, James M. Phang, Olga Zabirnyk

Chapter 19 Methods to Monitor Chaperone‐Mediated Autophagy Review Article
Pages 297-324
Susmita Kaushik, Ana Maria Cuervo

Chapter 20 Methods to Monitor Autophagy of Salmonella enterica serovar Typhimurium Review Article
Pages 325-343
Cheryl L. Birmingham, John H. Brumell

Chapter 21 Monitoring Autophagy during Mycobacterium tuberculosis Infection Review Article
Pages 345-361
Marisa Ponpuak, Monica A. Delgado, Rasha A. Elmaoued, Vojo Deretic

Chapter 22 Streptococcus‐, Shigella‐, and Listeria‐Induced Autophagy Review Article
Pages 363-381
Michinaga Ogawa, Ichiro Nakagawa, Yuko Yoshikawa, Torsten Hain, Trinad Chakraborty, Chihiro Sasakawa

Chapter 23 Kinetic Analysis of Autophagosome Formation and Turnover in Primary Mouse Macrophages Review Article
Pages 383-402
Michele S. Swanson, Brenda G. Byrne, Jean‐Francois Dubuisson

Chapter 24 Monitoring Macroautophagy by Major Histocompatibility Complex Class II Presentation of Targeted Antigens Review Article
Pages 403-421
Monique GannagГ©, Christian MГјnz

Chapter 25 Detachment‐Induced Autophagy In Three‐Dimensional Epithelial Cell Cultures Review Article
Pages 423-439
Jayanta Debnath

Chapter 26 Methods for Inducing and Monitoring Liver Autophagy Relative to Aging and Antiaging Caloric Restriction in Rats Review Article
Pages 441-455
Alessio Donati, Gabriella Cavallini, Ettore Bergamini

Chapter 27 Physiological Autophagy in the Syrian Hamster Harderian Gland Review Article
Pages 457-476
Ignacio Vega‐Naredo*, Ana Coto‐Montes

Author Index
Pages 477-497

Subject Index
Pages 499-508