Odontogenesis: Methods and Protocols

Front Matter ....Pages i-xviii
Front Matter ....Pages 1-1
 Microdissection and Isolation of Mouse Dental Epithelial Cells of Continuously Growing Mouse Incisors  (Hidemitsu Harada, Keishi Otsu)....Pages 3-11
 Establishment of an Immortalized Mouse Bmp2 Knockout Dental Papilla Mesenchymal Cell Line  (Wen’an Xu, Shuo Chen)....Pages 13-19
 Establishment of Stable Cell Lines from Primary Human Dental Pulp Stem Cells  (Elizabeth Guirado, Youbin Zhang, Anne George)....Pages 21-27
 Isolation of Dental Stem Cell-Enriched Populations from Continuously Growing Mouse Incisors  (Anamaria Balic)....Pages 29-37
 Application of Cell Lineage Tracing Combined with Immunofluorescence in the Study of Dentinogenesis  (Yan Jing, Chaoyuan Li, Jian Q. Feng)....Pages 39-48
 Tissue Recombination and Kidney Capsule Transplantation Assays for the Study of Epithelial-Mesenchymal Interactions  (Lucia Jimenez-Rojo, Thimios A. Mitsiadis)....Pages 49-55
Front Matter ....Pages 57-57
 Dental Mesenchymal Stem Cells: Dental Pulp Stem Cells, Periodontal Ligament Stem Cells, Apical Papilla Stem Cells, and Primary Teeth Stem Cells—Isolation, Characterization, and Expansion for Tissue Engineering  (Mey Al-Habib, George T. -J. Huang)....Pages 59-76
 Phenotypic Identification of Dental Pulp Mesenchymal Stem/Stromal Cells Subpopulations with Multiparametric Flow Cytometry  (Maxime Ducret, Jean-Christophe Farges, Marielle Pasdeloup, Emeline Perrier-Groult, Andreas Mueller, Frédéric Mallein-Gerin et al.)....Pages 77-90
 Dental Pulp Stem Cells: Isolation, Characterization, Expansion, and Odontoblast Differentiation for Tissue Engineering  (Qing Dong, Yuanyuan Wang, Fatemeh Mohabatpour, Li Zheng, Silvana Papagerakis, Daniel Chen et al.)....Pages 91-101
 In Vitro Analysis of Intramolecular Signaling Events in PDLSCs Using Confocal and TIRF Microscopy  (Annette Merkel, Anne George)....Pages 103-110
 A Mouse Model to Study Reparative Dentinogenesis  (R. C. Babb, D. Chandrasekaran, L. K. Zaugg, P. T. Sharpe)....Pages 111-119
 Multiwalled Carbon Nanotubes for Dental Applications  (Petros Kechagioglou, Eleftherios Andriotis, Petros Papagerakis, Silvana Papagerakis)....Pages 121-128
 Peptide-Mediated Biomimetic Regrowth of Human Enamel In Situ  (Kaushik Mukherjee, Qichao Ruan, Janet Moradian-Oldak)....Pages 129-138
 Bioengineering Tooth Bud Constructs Using GelMA Hydrogel  (Elizabeth E. Smith, Pamela C. Yelick)....Pages 139-150
 Whole-Mount In Situ Hybridization of Mouse Embryos Using DIG-Labeled RNA Probes  (Jingyi Wu, Xiaofang Wang)....Pages 151-159
Front Matter ....Pages 161-161
 In Situ Hybridization on Mouse Paraffin Sections Using DIG-Labeled RNA Probes  (Jingyi Wu, Jian Q. Feng, Xiaofang Wang)....Pages 163-171
 Methods for In Situ Protein Visualization in Dental Mineralized Tissues  (D. Hotton, A. Berdal, A. Bolaños)....Pages 173-180
 In Situ Hybridization in Mineralized Tissues: The Added Value of LNA Probes for RNA Detection  (G. Lignon, D. Hotton, A. Berdal, A. Bolaños)....Pages 181-190
 Immunofluorescence Procedure for Developing Enamel Tissues  (Xu Yang, Elia Beniash)....Pages 191-196
 Silver-Albumin Tissue Staining Protocol to Visualize Odontogenesis in Whole Embryos  (Julia C. Boughner, David M. L. Cooper)....Pages 197-210
 Isolation of SIBLING Proteins from Bone and Dentin Matrices  (Jingyi Wu, Xiaofang Wang)....Pages 211-218
 Immunohistochemical Co-Localization of Amelogenin and Ameloblastin in Developing Enamel Matrix  (Rucha Arun Bapat, Janet Moradian-Oldak)....Pages 219-228
 The Expression and Purification of Recombinant Mouse Ameloblastin in E. coli  (Jingtan Su, Rucha Arun Bapat, Janet Moradian-Oldak)....Pages 229-236
Front Matter ....Pages 237-237
 Protocols for Studying Formation and Mineralization of Dental Tissues In Vivo: Extraction Protocol for Isolating Dentin Matrix Proteins from Developing Teeth  (Yasuo Yamakoshi, Jan C.-C. Hu, Mari M. Saito, James P. Simmer)....Pages 239-250
 Purification of Developing Enamel Matrix Proteins Using Preparative SDS-PAGE  (Steven J. Brookes, Claire M. Gabe)....Pages 251-265
 Using ImageJ (Fiji) to Analyze and Present X-Ray CT Images of Enamel  (Steven J. Brookes)....Pages 267-291
 Scanning Electron Microscopy (SEM) Methods for Dental Enamel  (Steinar Risnes, Muhammad Saeed, Amer Sehic)....Pages 293-308
 Microcomputed Tomography Imaging in Odontogenesis Studies  (Kostas Verdelis, Phil Salmon)....Pages 309-324
 Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM) for the Examination of Dental Hard Tissues  (Victor E. Arana-Chavez, Leticia S. Castro-Filice)....Pages 325-332
Front Matter ....Pages 333-333
 Rodent Dental Fluorosis Model: Extraction of Enamel Organ from Rat Incisors  (M. Suzuki, J. D. Bartlett)....Pages 335-340
 Three-Dimensional Assessment of Crown Size and Eruption Space for Developing Third Molars: Data Collection Techniques Based on Cone-Beam Computed Tomography (CBCT)  (D. F. Marchiori, G. V. Packota, J. C. Boughner)....Pages 341-356
 Protocols to Study Dental Caries In Vitro: Microbial Caries Models  (Bennett T. Amaechi, Livia M. A. Tenuta, Antonio P. Ricomini Filho, Jaime A. Cury)....Pages 357-368
 In Vitro Caries Models for the Assessment of Novel Restorative Materials  (Basma Sulaiman Ghandourah, Anna Lefkelidou, Raed Said, Xanthippi Chatzistavrou, Susan Flannagan, Carlos Gonzáles-Cabezas et al.)....Pages 369-377
 Protocols to Study Dental Caries In Vitro: pH Cycling Models  (Bennett T. Amaechi)....Pages 379-392
 In Vivo Rodent Models for Studying Dental Caries and Pulp Disease  (June Hsiao, Yuanyuan Wang, Li Zheng, Ruirui Liu, Raed Said, Lubomir Hadjiyski et al.)....Pages 393-403
Front Matter ....Pages 405-405
 Protocol GenoDENT: Implementation of a New NGS Panel for Molecular Diagnosis of Genetic Disorders with Orodental Involvement  (Tristan Rey, Julien Tarabeux, Bénédicte Gerard, Marion Delbarre, Antony Le Béchec, Corinne Stoetzel et al.)....Pages 407-452
 Protocols for Genetic and Epigenetic Studies of Rare Diseases Affecting Dental Tissues  (Bruna Rabelo Amorim, Pollyanna Almeida Costa dos Santos, Caroline Lourenço de Lima, Denise Carleto Andia, Juliana Forte Mazzeu, Ana Carolina Acevedo)....Pages 453-492
 Protocols, Methods, and Tools for Genome-Wide Association Studies (GWAS) of Dental Traits  (Cary S. Agler, Dmitry Shungin, Andrea G. Ferreira Zandoná, Paige Schmadeke, Patricia V. Basta, Jason Luo et al.)....Pages 493-509
 Measurement of Early Childhood Oral Health for Research Purposes: Dental Caries Experience and Developmental Defects of the Enamel in the Primary Dentition  (Jeannie Ginnis, Andrea G. Ferreira Zandoná, Gary D. Slade, John Cantrell, Mikafui E. Antonio, Bhavna T. Pahel et al.)....Pages 511-523
 The Supragingival Biofilm in Early Childhood Caries: Clinical and Laboratory Protocols and Bioinformatics Pipelines Supporting Metagenomics, Metatranscriptomics, and Metabolomics Studies of the Oral Microbiome  (Kimon Divaris, Dmitry Shungin, Adaris Rodríguez-Cortés, Patricia V. Basta, Jeff Roach, Hunyong Cho et al.)....Pages 525-548
 Saliva and Gingival Crevicular Fluid (GCF) Collection for Biomarker Screening  (Petros Papagerakis, Li Zheng, Doohak Kim, Raed Said, Amber A. Ehlert, Kevin K. M. Chung et al.)....Pages 549-562
Back Matter ....Pages 563-565