New Trends in Enzyme Catalysis and Biomimetic Chemical Reactions

Cover Page......Page 1
Title Page......Page 4
ISBN 1402010060......Page 5
Contents......Page 6
Preface......Page 10
1.1.1 X-ray structural analysis......Page 12
1.1.2 Infrared, Raman and light absorptin spectroscopy......Page 13
1.1.3 Fluoresecence and phosphorescence......Page 17
1.1.4 Flourescence-photochrome labeling techniques......Page 22
1.1.5 Electron spin resonance (ESR)......Page 25
1.1.6 Nuclear magnetic resonance......Page 32
1.1.7 Mass spectrometry (MS)......Page 37
1.2.1 Kinetic isotope effect (KIE)......Page 39
1.2.2 Transition state analogues methods......Page 42
1.2.3 Nano-second temperature jump......Page 44
2.1 General principles of enzymatic catalysis......Page 46
2.2 Electron transfer (ET)......Page 47
2.2.1 Theoretical models......Page 48
2.2.2 Experimental data......Page 60
2.3 Hydrogen transfer......Page 66
2.3.1 Theoretical grounds......Page 67
2.3.2 Experimental data......Page 70
2.4 Electron-proton coupling. Mechanism of ATPase reactions in energy-conversion systems......Page 71
2.5.1 Synchronization factor......Page 74
2.5.2.. The principle of “optimum motion” in elementary acts of chemical and enzymatic processes......Page 76
2.6 Multi-electron mechanisms of redox reactions. Switching molecular devices......Page 77
2.7 Stabilization of enzyme reactions transition states......Page 78
2.8 Pretransition states......Page 81
2.9 Principle of “optimum motion” and mechanisms of enzymes reactions......Page 82
2.10 Radical mechanisms of enzyme catalysis......Page 86
2.11 Substrate channeling......Page 87
2.12 Relationships between the energy and entropy activation of enzymatic processes......Page 88
3.1.1 Overview......Page 92
3.1.2 Structure and physicochemical properties of the nitrogenase active sites......Page 93
3.1.3 Kinetics and mechanism of the nitrogenase reaction......Page 97
3.1.4 ATP centers and ATP hydrolysis......Page 100
3.1.5 Dinitrogen reduction......Page 102
3.2.1 Overview......Page 108
3.2.2 Energy of hydroxylation reaction......Page 109
3.2.3 Structure of cytochrome P450 active site......Page 110
3.2.4 Mechanism of the cytochrome P450 catalyzed reactions......Page 111
3.3.1 Overview......Page 119
3.3.2 Structure of MMOH active site......Page 120
3.3.3 Mechanism of hydroxylation catalyzed by the MMO complex......Page 122
3.4 Nitric oxide synthase......Page 124
3.5.1 Reaction centers from photosynthetic bacreria......Page 126
3.5.2 Plant photosynthesis......Page 136
4.1.1 Overview......Page 143
4.1.2 Low-temperature protein dynamics......Page 145
4.1.3 Protein dynamics at ambient temperature......Page 147
4.1.4 Dynamics of enzymes active sites......Page 150
4.1.5 Simulation of protein molecular dynamics......Page 151
4.1.6 Mechanisms of proteins intramolecular dynamics......Page 153
4.1.7 Protein dynamics and their functional activity......Page 157
4.2.1 Theoretical calculations......Page 160
4.2.2 Experimental approaches......Page 161
4.3.1 Overview......Page 168
4.3.2 β-glycosidase Sulfolobus solfataricus......Page 169
5.1 Antibody catalysis......Page 175
5.2 Enzymes in organic solvents......Page 177
5.3 Enzymes in synthetic chemistry......Page 178
5.4 Enzymes design and redesign......Page 180
6.1 General principles......Page 183
6.2 Reduction of dinitrogen......Page 184
6.3 Hydroxylation of organic compounds......Page 186
6.4 Light energy conversion......Page 190
6.5 Water oxidation......Page 195
6.6 Organic reactions......Page 196
References......Page 200
G......Page 239
Q......Page 240
X......Page 241