New Developments and New Applications in Animal Cell Technology: Proceedings of the 15th ESACT Meeting

Genetic Manipulation of the Mammalian Genome....Pages 1-3
Engineering Glycosylation in Animal Cells....Pages 5-23
Sessions on: Biosynthesis and Post-Translational Modifications of Recombinant Proteins, and Cell Physiology and Metabolic Engineering of Animal Cells....Pages 25-26
Front Matter....Pages 27-27
Enhanced Recombinant Protein Expression in Insect Cells in the Presence of Dimethylsulfoxide (DMSO)....Pages 29-33
Studies of the Hepatitis C Virus Recombinant Protein Production and Its Effects on SF-9 Insect Cells Metabolism....Pages 35-37
The Human Multidrug Transporter (MDR1) Expressed in the Baculovirus-SF9 Insect Cell System....Pages 39-41
Glutamine Synthetase Transfected Cells May Avoid Selection by Releasing Glutamine....Pages 43-49
Study of Different Cell Culture Conditions for the Production of a “Reshaped” Mab in NSO Cells....Pages 51-53
Increasing Monoclonal Antibody Productivity by Semicontinuous Substitution of Production Medium for Growth Medium....Pages 55-57
Expression of Recombinant Human Insulin in Chinese Hamster Ovary Cells is Complicated by Intracellular Insulin-Degrading Enzymes....Pages 59-67
High Yield Expression of Recombinant Plasma Factors: Use of Recombinant Endoprotease Derivatives In Vivo and In Vitro....Pages 69-76
The Role of Cell Cycle in Determining Levels of Gene Expression in Cho Cells....Pages 77-79
Heterogeneity within DHFR-Mediated Gene Amplified Population of Cho Cells Producting Chimeric Antibodies....Pages 81-83
Immunofluorescence Detection of Recombinant Monoclonal Antibody as a Tool in the Characterization of Chinese Hamster Ovary Cell Lines....Pages 85-92
Electrolyzed Reduced Water Which Can Scavenge Active Oxygen Species Supresses Cell Growth and Regulates Gene Expression of Animal Cells....Pages 93-96
Differential Gene Expression of Cytochrome P450 in Immortalised Hepatocyte Cell Lines....Pages 97-99
Post-Transcriptional Control of Recombinant Gene Expression: mRNA Retargeting and Regulation of Expression....Pages 101-103
Transient Transfection in Mammalian Cells....Pages 105-112
Large Scale Transient Gene Expression in Mammalian Cells....Pages 113-116
Synergistic Enhancement of Transient Expression by Dioleoyl-Melittin (DOM) and Polyethylenimine (PEI) in Mammalian Cells in Suspensionculture....Pages 117-120
Front Matter....Pages 27-27
Transient Expression of a Soluble and Secreted form of Heterodimeric T-Cell Receptor in Hek-293....Pages 121-123
Measurable Parameters of Cells and Precipitate Predict Transfectability with Calcium Phosphate....Pages 125-128
Front Matter....Pages 129-129
Influence of Na-Butyrate on the Production and Sialylation of Human Interferon-γ by α2,6-Sialyltransferase Engineered CHO-Cells....Pages 131-133
Elevated pCO 2 Inhibits the Polysialylation of the Neural Cell Adhesion Molecule in CHO MT2-1-8 Cell Cultures....Pages 135-140
Influence of Cultivation Conditions on Glycosylation Pattern-a Fed-Batch and Continuous Culture Study....Pages 141-147
Effects of Different Production Systems on Glycosylation Pattern of Murine Monoclonal IgA....Pages 149-152
Identification of Altered Glycosylation as the Major Difference Between Intracellulary Accumulated and Secreted β-Trace Protein Produced in Baculovirus-Infected Insect Cells....Pages 153-155
How Ammonium Dominates the Metabolism of in Vitro Cultivated Mammalian Cells....Pages 157-161
Study of Human Recombinant GM-CSF Produced in Different Host Systems Using Monoclonal Antibodies....Pages 163-165
Front Matter....Pages 166-166
Modification of Hybridoma Cells Metabolism....Pages 167-174
Cloning and Expression of a Cytosolic Sialidase from CHO Cells in a Glutathione S-Tramsferase (GST)-Encoding Expression Vector....Pages 175-179
Construction of a Novel CHO Cell Line Coexpressing Human GlycosyItransferases and fusion PSGL-1-Immunoglobulin G....Pages 181-183
Production of Defined Lycosylation Variants of Secreted Human Glycoprotein Therapeutics by Coexpression with Human Recombinant Glycosyl Transferases in BHK-21 Cells....Pages 185-190
Antisense RNA for the Elimination of Neugc Rresidues from Recombinant Glycoproteins....Pages 191-193
Front Matter....Pages 195-195
Intracellular Fatty Acid Composition Affects Cell Yield, Energy Metabolism and Cell Damage in Agitated Cultures....Pages 197-203
Lipid Requirements of a Recombinant Chinese Hamster Ovary Cell Line (CHO)....Pages 205-207
Sustained Expression in Proliferation Controled BHK-21 Cells....Pages 209-214
Cell Growth Inhibition by the IRF-1 System....Pages 215-217
Correlation between BHK Cell Specific Productivity and Metabolism....Pages 219-221
Effect of Growth Arrest in BHK Metabolism: On Line Monitoring by 13 C and 31 P NMR Spectroscopy....Pages 223-225
Front Matter....Pages 195-195
Cell Survival in CHO Cell Cultures Grown in a Defined Protein Free Medium....Pages 227-229
Enhanced Apoptosis in Insect Cells Ciltivated in Simulated Microgravity....Pages 231-233
Modulation of Apoptosis by BCL-2 Expression Following Amino Acid Deprivation and in High Cell Density Perfusion Cultures....Pages 235-241
Effect of BCL-2 Expression on Hybridoma Cell Growth During Stressful Conditions....Pages 243-245
Inhibition of c-jun Expression in F-Mel Cells Causes Cell Cycle Arrest and prevention of Apoptosis....Pages 247-254
Hippocampal Cells in Culture as a Model to Study Neuronal Apoptosis....Pages 255-258
Development of Carboxy SNARF-1-AM and Annexin V Assays for the Determination of Apoptosis in Heterogeneous Cultures....Pages 259-261
Session on Integrated Bioprocessing in Animal Cell Technology....Pages 263-264
Front Matter....Pages 265-265
Diauxic Cell Behavior Enables Detoxification of CHO Cell Culture Medium During Fed Batch Cultivation....Pages 267-271
Effects of Polysaccharide Derived from Tea on Growth of Human Cell Lines in Serum Free Culture....Pages 273-275
Metabolic Demands of Bti-TN-5B1-4 (High Five TM ) Insect Cells During Growth and After Infection with Baculovirus....Pages 277-279
Effects of Ammonium and Lactate During Continuous Hybridoma Fermentations In A Fluidized-Bed Bioreactor....Pages 281-283
Serum Concentration and pH Affect the CD13 Receptor Content of HL60 Cells Cultured in Stirred Bioreactors....Pages 285-291
Four Regulation-Friendly Serum-Free Media For Mammalian and Insect Cells....Pages 293-302
Proteolytic Activities in the Baculovirus-Insect Cell Expression System....Pages 303-306
Front Matter....Pages 307-307
Development of Proteinase Assays for Improved Cho Cell Cultures....Pages 309-316
Digital Image Analysis: Quantitative Evaluation of Colored Microscopic Images of Animal Cells....Pages 317-319
The Viable Cell Monitor: A Dielectric spectroscope for Growth and Metabolic Studies of Animal Cells on Macroporous Beads....Pages 321-328
Measurements of Changes in Cell Size Distribution to Monitor Baculovirus Infection of Insect Cells....Pages 329-331
Dead Cell Estimation — a Comparison of Different Methods....Pages 333-336
Front Matter....Pages 307-307
Fed-Batch Culture Development Based on Biomass Monitoring....Pages 337-342
On-Line Monitoring of Protein and Susbtrate/Product Concentration in Mammalian Cell Cultivation Process....Pages 343-346
Process Control and On-Line Feeding Strategies for Fed-Batch and Dialysis Cultures of Hybridoma Cells....Pages 347-349
Metabolic Network Analysis of a Hybridoma Cell Line Using Mass Balances and C 14 -Labelled Glucose and Glutamine....Pages 351-353
Dynamic Medium Optimization by On-Line Heat Flux Measurement and a Stoichiometric Model in Mammalian Cell Culture....Pages 355-357
Modeling of Glycoprotein Production by Chinese Hamster Ovary Cells for Process Monitoring and Control....Pages 359-361
The Temperature Effect in Mammalian Cell Culture an Arrhenius Interprettion....Pages 363-366
Front Matter....Pages 367-367
Dielectrophoretic Forces Can be Exploited to Increase the Efficiency of Animal Cell Perfusion Cultures....Pages 369-375
Use of A New Microcarrier with Two-Dimensional Geometry for the Culture of Anchorage-Dependent Cells in Sonoperfused, Continuously Stirred Tank Reactors....Pages 377-380
Evaluation of Porous Microcarriers in Fluidized Bed Reactor for Protein Production by Heik 293 Cells....Pages 381-384
Fluidized Bed Technology: Influence of Fluidization Velocity on Nutrient Consumption and Product Expression....Pages 385-387
High Density and Scale-up Cultivation of Recombinant Cho Cell Line and Hybridomas with Porous Microcarrier Cytopore....Pages 389-393
Cell-Settler Perfusion System for the Production and Glycosylation of Human Interferon-γ by Clumped Cells....Pages 395-397
Disposable Bioreactor for Cell Culture Using Wave-Induced Agitation....Pages 399-407
Use of miniPerm System for an Efficient Production of Mouse Monoclonal Antibodies....Pages 409-415
Production of Anti-EGF Receptor hMAB in Hollow Fiber Bioreactors for in Vivo Diagnosis....Pages 417-419
Optimisation of the Production of VLP’s in 2 Lt Stirred Bioreactors....Pages 421-427
Direct Capture of Monoclonal Antibodies with a New High Capacity STREAMLINE SP XL Ion Exchanger....Pages 429-431
The Effect of Different Purification Schemes on the Activity of a Monoclonal Antibody....Pages 433-435
Interfacing of Protein Product Recovery with an Insect Cell Baculovirus Production System....Pages 437-439
Front Matter....Pages 367-367
Preparation of Plasmid DNA Using Displacement Chromatography....Pages 441-443
Novel Methods for Large-Scale Preparation of Nutrient Media and Buffered Salt Solutions....Pages 445-448
Large Scale Application of the Semliki Forest Virus System: 5-HT3 Receptor Production....Pages 449-455
From Applied Research to Industrial Application: the Success Story of Monitoring Intracellular Ribonucleotide Pools....Pages 457-462
New Technology of Production Recombinant Human Erythropoietin for Peroral Administration....Pages 463-465
Front Matter....Pages 467-467
Significance of Multiple Testing on Murine Leukemia Virus of Mouse Hybridomas....Pages 469-472
Murine Retrovirus Detection Using Mus Dunni Cells and S + L − Cells....Pages 473-480
The Removal of Model Viruses During the Purification of Human Albumin Using Chromatographic Procedures....Pages 481-483
Inactivation of Hepatitis A Virus During the Production of Nordimmun....Pages 485-490
Session on: Viral Vector Production for Gene Therapy....Pages 491-491
Adeno-Associated Viral Vectors: Principles and in Vivo Use....Pages 493-502
Novel Retroviral Packaging Cell Lines: Improved Vector Production for Efficient Hematopoietic Progenitor Cell Transduction....Pages 503-508
Scale-Up and Optimisation Issues in Gene Therapy Viral Vector Production....Pages 509-513
Comparison of Manufacturing Techniques for Adenovirus Production....Pages 515-521
Safety Considerations in the Development of New Retroviral and Adenoviral Vectors for Gene Therapy....Pages 523-529
Gene Therapy for Neuromuscular Disorders: Macrophages as Shuttles for Widespread Targeting....Pages 531-539
Gene Transfer into Dog Myoblasts....Pages 541-543
Biodistribution Analysis of a Gene Therapy Vector Using the Polymerase Chain Reaction (PCR) Technique....Pages 545-548
Session on....Pages 549-549
The Production of Influenza Virus by Immobilised MDCK Cells....Pages 551-554
Front Matter....Pages 467-467
Serum-Free Grown MDCK Cells: An Alternative for Influenza Vaccine Virus Production....Pages 555-559
Evaluation of the New Medium (MDSS2N), Free of Serum and Animal Proteins, for the Production of Biologicals....Pages 561-568
Production of High Titre Disabled Infectious Single Cycle (Disc) HSV-2 from a Microcarrier Culture....Pages 569-571
New Form of the Live Measles Vaccine for Oral Administration....Pages 573-575
Study of Leningrad-16 Vaccine Strain of Measles Virus Reproduction in Cell Cultures Perspective for Biotechnology....Pages 577-579
Recent Advances with New Vaccine Adjuvants: From Preclinical to Clinical Development....Pages 581-582
Vaccination with Recombinant Suicidal DNA/RNA....Pages 583-591
Growth of Goat Endothelial Cells for the Production of A Veterinary Vaccine....Pages 593-595
Expression in Insect Cells of the Major Parasite Antigen Associated with Human Resistance to Schistosomiasis....Pages 597-600
Modulation of CD4 Expression on Helper T Lymphocytes and U937 Cells by Ganglioside GM3 and Its Derivatives....Pages 601-605
Human Serum in Leukocyte Cultures Producing Human Interferon Alpha....Pages 607-612
The New Type of Immunomodulator....Pages 613-615
 In Vitro Immunization of Human Peripheral Blood Lymphocytes with Cholera Toxin B Subunit....Pages 617-623
Session on:....Pages 625-625
Analysis of Cell Growth in A Fixed Bed Bioreactor Using Magnetic Resonance Spectroscopy and Imaging....Pages 627-633
Expansion of Human Hematopoietic Progenitor Cells in A Fixed Bed Bioreactor....Pages 635-636
A Novel Assay to Determine and Quantify the Regulation of Cell Motility and Migration Demonstrated on Hematopotetic Cells....Pages 637-643
Immortalization of Differentiated Hepatocytes....Pages 645-655
Fixed-Bed Reactors for Animal Cell Cultivation: An Approach to Artificial Organs....Pages 657-659
High Density Perfusion Culture of Primary Rat Hepatocytes for Potential Use as a Bioartificial Liver Device....Pages 661-663
Front Matter....Pages 467-467
Metabolic Competence and Hormonal Regulation of Primary Porcine Hepatocytes in a 3-D Sandwich Configuration....Pages 665-667
Novel Mini-Bioreactors for Islet Cell Culture....Pages 669-672
Cultivation of Skin Cells Suitable for Recovery of Burn Wounds....Pages 673-675
Tissue Therapy for Treatment of Primary Myodystrophies....Pages 677-679
Possibility of Application of Thyroid Organ Culture for the Treatment of Persistent Hypothyroidism....Pages 681-683
Human Cells as Therapeutic Agents....Pages 685-686
Session on....Pages 687-687
Embryonic Stem Cell Differentiation Models: Cardiovascular, Myogenic and Neurogenic Development In Vitro ....Pages 689-703
Responses of Human Lung Epithelial Cells (A549) to Pathogenic Infection by Mycoplasma Pneumoniae ....Pages 705-711
Polar Lipid Profiling of Mycoplasma Pneumoniae -Infected Human Lung Epithelial Cells....Pages 713-715
Comparison of Three Dimensional (3-D) Rat Hepatocyte Cultures in Simulated Microgravity Conditions....Pages 717-719
High Density Culture of the Human Hepatoma Cell Line HepG2: Long-Term Culture for In Vitro Toxicology....Pages 721-724
Application of Primary Cultures of Rat Fetal Neurons to the Study of Neurotrophic Action of Peptides....Pages 725-727
Development and Validation of an Image Analysis System for Single Cell Characterization in Cell Monolayers....Pages 729-731
Development of an Optically Accessible Perfusion Chamber for In Situ Assays and for Long-term Cultivation of Mammalian Cells....Pages 733-736
Analysis of Mitogenic Activity of Proteins after Separation by Gel Electrophoresis....Pages 737-739
Workshop on: The Use of Animal Cells Versus the Use of Transgenic Animals for the Production of Recombinant Proteins....Pages 741-741
Secretion of Fusion Proteins into Milk by Transgenic Mouse Mammary Epithelium....Pages 743-743
The Production of Proteins in the Milk of Transgenic Livestock: A Comparison of Micro Injection and Nuclear Transfer....Pages 745-750
Creation of Mice Expressing Human Antibody by Introduction of a Human Chromosome....Pages 751-756
Front Matter....Pages 467-467
Transgenic Technology-A Challenge for Mammalian Cell Culture Production Systems....Pages 757-763