Neurotrophic Factors: Methods and Protocols

Front Matter ....Pages i-xv
 Neurotrophic Factors: An Overview  (Stephen D. Skaper)....Pages 1-17
 Ocular Nerve Growth Factor (NGF) and NGF Eye Drop Application as Paradigms to Investigate NGF Neuroprotective and Reparative Actions  (Paola Tirassa, Pamela Rosso, Angela Iannitelli)....Pages 19-38
 Culture of Rodent Cortical, Hippocampal, and Striatal Neurons  (Laura Facci, Stephen D. Skaper)....Pages 39-47
 Culture of Neonatal Rodent Microglia, Astrocytes, and Oligodendrocytes from the Cortex, Spinal Cord, and Cerebellum  (Stephen D. Skaper, Laura Facci)....Pages 49-61
 Central Nervous System Neuron-Glia co-Culture Models and Application to Neuroprotective Agents  (Stephen D. Skaper, Laura Facci)....Pages 63-80
 Preparation of Adult Rat Sensory Neuron Cultures and Their Application to Growth Cone Turning Assays  (Christine A. Webber, Douglas W. Zochodne)....Pages 81-92
 Sensory Neurons from Tau Transgenic Mice and Their Utility in Drug Screening  (Aviva M. Tolkovsky, Jack Brelstaff)....Pages 93-105
 Culture of Rat Mesencephalic Dopaminergic Neurons and Application to Neurotoxic and Neuroprotective Agents  (Stephen D. Skaper, Massimo Barbierato, Vanni Ferrari, Morena Zusso, Laura Facci)....Pages 107-118
 Culture of Neurons and Smooth Muscle Cells from the Myenteric Plexus of Adult Mice  (Paola Brun, Hamid I. Akbarali)....Pages 119-125
 Astrocyte/Microglia Cocultures as a Model to Study Neuroinflammation  (Laura Facci, Massimo Barbierato, Stephen D. Skaper)....Pages 127-137
 Micropatterned Geometry Shape Oligodendrocyte and Microglia Plasticity  (Cinzia Volonté, Adele De Ninno, Susanna Amadio)....Pages 139-154
 Oligodendrocyte Progenitor Cell Cultures: A Model to Screen Neurotrophic Compounds for Myelin Repair  (Massimo Barbierato, Stephen D. Skaper, Laura Facci)....Pages 155-166
 Cell Enumeration Assays: Application of the MTT and Sulforhodamine B Assays to Lipopolysaccharide-Stimulated Neonatal Rodent Microglia  (Laura Facci, Stephen D. Skaper)....Pages 167-178
 Diolistic Labeling and Analysis of Dendritic Spines  (M. Foster Olive, Armani P. Del Franco, Cassandra D. Gipson)....Pages 179-200
 Culture of Mouse Giant Central Nervous System Synapses and Application for Imaging and Electrophysiological Analyses  (Dimitar Dimitrov, Laurent Guillaud, Kohgaku Eguchi, Tomoyuki Takahashi)....Pages 201-215
 Methods for Quantitative Analysis of Axonal Cargo Transport  (Matias Alloatti, Luciana Bruno, Tomas L. Falzone)....Pages 217-226
 Neuronal Cell Morphology in Primary Cerebellar Granule Cells Using High-Content Analysis  (Nicholas Radio, Steven Frank)....Pages 227-237
 Nerve Growth Factor-Induced Angiogenesis: 1. Endothelial Cell Tube Formation Assay  (Philip Lazarovici, Adi Lahiani, Galit Gincberg, Dikla Haham, Arnon Fluksman, Ofra Benny et al.)....Pages 239-250
 Nerve Growth Factor-Induced Angiogenesis: 2. The Quail Chorioallantoic Membrane Assay  (Philip Lazarovici, Adi Lahiani, Galit Gincberg, Dikla Haham, Cezary Marcinkiewicz, Peter I. Lelkes)....Pages 251-259
 Rat Pancreatic Beta-Cell Culture  (Myrian Velasco, Carlos Larqué, Carlos Manlio Díaz-García, Carmen Sanchez-Soto, Marcia Hiriart)....Pages 261-273
 Growth and Neurotrophic Factors in Embryonic Stem Cells  (Micaela Pannella, Luciana Giardino, Laura Calzà, Mercedes Fernández)....Pages 275-294
 Site-Specific Direct Labeling of Neurotrophins and Their Receptors: From Biochemistry to Advanced Imaging Applications  (Francesco Gobbo, Fulvio Bonsignore, Rosy Amodeo, Antonino Cattaneo, Laura Marchetti)....Pages 295-314
 Isolation and Culture of Brain Microvascular Endothelial Cells for In Vitro Blood-Brain Barrier Studies  (Hector Rosas-Hernandez, Elvis Cuevas, Susan M. Lantz, Merle G. Paule, Syed F. Ali)....Pages 315-331
 Preparation of Rat Hippocampal Organotypic Cultures and Application to Study Amyloid β-Peptide Toxicity  (Maria Rosanna Bronzuoli, Roberta Facchinetti, Caterina Scuderi)....Pages 333-341
 An Animal Model of Alzheimer Disease Based on the Intrahippocampal Injection of Amyloid β-Peptide (1–42)  (Roberta Facchinetti, Maria Rosanna Bronzuoli, Caterina Scuderi)....Pages 343-352
 Active Induction of Experimental Autoimmune Encephalomyelitis in C57BL/6 Mice  (Gabriella Contarini, Pietro Giusti, Stephen D. Skaper)....Pages 353-360
 A Model of Systemic Inflammation to Study Neuroinflammation  (Vincenzo Sorrenti, Pietro Giusti, Morena Zusso)....Pages 361-372
 Spared Nerve Injury as a Long-Lasting Model of Neuropathic Pain  (Serena Boccella, Francesca Guida, Enza Palazzo, Ida Marabese, Vito de Novellis, Sabatino Maione et al.)....Pages 373-378
 An In Vivo Compression Model of Spinal Cord Injury  (Irene Paterniti, Emanuela Esposito, Salvatore Cuzzocrea)....Pages 379-384
 A Controlled Cortical Impact Preclinical Model of Traumatic Brain Injury  (Michela Campolo, Emanuela Esposito, Salvatore Cuzzocrea)....Pages 385-391
 Middle Cerebral Artery Occlusion by an Intraluminal Suture Method  (Rosalia Crupi, Rosanna Di Paola, Emanuela Esposito, Salvatore Cuzzocrea)....Pages 393-401
 Primary Motor Neuron Culture to Promote Cellular Viability and Myelination  (Jun-Kyo Francis Suh, Sujin Hyung)....Pages 403-411
 Culture of Human Primary Bone Cells and Phenotype Assessment  (Paola Brun)....Pages 413-421
 Recreating Intestinal Peristalsis in the Petri Dish  (Rosa Espinosa-Luna, Alma R. Barajas-Espinosa, Fernando Ochoa-Cortez, Carlos Barajas-López)....Pages 423-432
 Synthesis of Biocompatible Titanate Nanofibers for Effective Delivery of Neuroprotective Agents  (Asya Ozkizilcik, Roger Williams, Z. Ryan Tian, Dafin F. Muresanu, Aruna Sharma, Hari S. Sharma)....Pages 433-442
 Preparation and Characterization of Biocompatible Chitosan Nanoparticles for Targeted Brain Delivery of Peptides  (Muge Yemisci, Secil Caban, Eduardo Fernandez-Megia, Yilmaz Capan, Patrick Couvreur, Turgay Dalkara)....Pages 443-454
Back Matter ....Pages 455-458