Neuropeptide Technology: Synthesis, Assay, Purification, and Processing

Content: 
Front Matter
Page iii

Copyright page
Page iv

Contributors to Volume 6
Pages ix-xii

Preface
Page xiii
P. MICHAEL CONN

Methods in Neurosciences
Page xv

1 - Large-Scale Synthesis of Gonadotropin-Releasing Hormone Antagonists for Clinical Investigations
Pages 3-27
Carl Hoeger, Paula Theobald, John Porter, Charleen Miller, Dean Kirby, Jean Rivier

2 - Synthesis of Peptide Substrates for Protein Kinase C
Pages 27-34
Akira Kishimoto, Ichiro Yasuda, Atsushi Sakurai, Hiroaki Aihara

3 - Synthesis of Glycosyl Neuropeptides
Pages 35-50
Josep L. Torres, Eduard Bardají, Gregorio Valencia

4 - Assays for Biosynthesis of Thyrotropin-Releasing Hormone
Pages 51-69
Eduardo A. Nillni, Kevin A. Sevarino, Ping Wu, Ivor M.D. Jackson

5 - Microsequence Analysis of Proteins Purified by Gel Electrophoresis
Pages 69-84
Wolfgang H. Fischer, David Karr, Brent Jackson, Minkyu Park, Wylie Vale

6 - Preparation of Site-Specific Peptide Immunogens Using Multiple Antigen Peptide Approach System
Pages 85-107
James P. Tam, Yi-An Lu

7 - Subcellular Distribution of Neuropeptides and Measurement of Their in Vitro Release
Pages 111-119
Margery C. Beinfeld

8 - Analysis of Tachykinin Peptide Family Gene Expression Patterns by Combined High-Performance Liquid Chromatography–Radioimmunoassay
Pages 119-130
Y. Takeda, J.D. Cremins, J. Takeda, J.E. Krause

9 - Ultrastructural Localization of Peptides: Comparison of Methods
Pages 130-147
Jean Y. Jew, Nathan B. Fountain, Evelyn Y. Jew

10 - Measurement of Immune System in Response to Peptides in Central Nervous System
Pages 147-157
Lewis D. Fannon, M. Ian Phillips

11 - Identification of Functionally Significant Phosphorylation Sites on Neuronal Proteins and Preparation of Antibodies That Recognize Them
Pages 158-176
Bruce L. Patton, Stephen G. Miller, Mary B. Kennedy

12 - Measurement of Brain Peptides: Angiotensin and Atrial Natriuretic Peptide in Tissue and Cell Culture
Pages 177-206
M. Ian Phillips, Birgitta Kimura, Mohan K. Raizada

13 - Regionally Specific Antisera to Human β-Preprotachykinin
Pages 207-221
J. Michael Conlon

14 - Measurement of Neurokinin B by Radioimmunoassay
Pages 221-231
J. Michael Conlon

15 - Radioimmunoassay of Tachykinins
Pages 232-247
Heng-Phon Too, John E. Maggio

16 - Radioimmunoassay of α-Melanocyte-Stimulating Hormone
Pages 247-260
Miklós Vecsernyés, János Julesz

17 - Radioimmunoassay of Atrial Natriuretic Peptide
Pages 260-271
Claudio Poiesi

18 - Radioimmunoassay of Cholecystokinin
Pages 271-281
Roger D. Reidelberger, Grace L. Rosenquist

19 - Secretion and Radioimmunoassay of Somatostatin: In Vitro System
Pages 281-293
Barthold Vonen, Jon Florholmen

20 - Purification and Characterization of Immunoregulatory Peptides from Neuroendocrine Tissues: Suppressin as a Model
Pages 297-306
J. Edwin Blalock, Robert D. LeBoeuf

21 - Purification and Characterization of Neuroendocrine Peptides from Rat Brain: Prosomatostatin Isolation
Pages 306-321
Günther Sperk, Georg Strieder

22 - Preparation of Glia Maturation Factor β
Pages 321-337
Ramon Lim, Asgar Zaheer

23 - Detection and Purification of Thyrotropin-Releasing Hormone Precursor Peptides Using Antisera Generated against Synthetic Peptides
Pages 337-354
Ping Wu, Ivor M.D. Jackson

24 - Physiologic Roles of Peptides as Central Nervous System Transmitters
Pages 354-369
Marvin R. Brown, Laurel A. Fisher

25 - Assays for Arginine/Lysine Carboxypeptidases: Carboxypeptidases H (E; Enkephalin Convertase), M, and N
Pages 373-385
Randal A. Skidgel

26 - Assay of Peptidase That Removes Carboxy-Terminal Tripeptide from 125I-Labeled Atrial Natriuretic Factor
Pages 386-397
Gibbes R. Johnson, Carolyn J. Foster

27 - Assays for Enzymes That Metabolize Atrial Natriuretic Peptide
Pages 397-410
Philip A. Krieter, Gillian M. Olins

Index
Pages 411-423