Neurobiology of Steroids

Content: 
Methods in Neurosciences
Page ii

Front Matter
Page iii

Copyright page
Page iv

Contributors to Volume 22
Pages ix-xiii

Preface
Page xv
E. RONALD DE KLOET, WIN SUTANTO

Methods in Neurosciences
Pages xvii-xviii

1 - Steroid Hormone Transport through Blood–Brain Barrier: Methods and Concepts
Pages 3-22
William M. Pardridge

2 - Enhanced Delivery of Steroids to the Brain Using a Redox-Based Chemical Delivery System
Pages 23-35
James W. Simpkins, Nicholas Bodor

3 - Neurosteroids: Biosynthesis and Function
Pages 36-50
Paul Robel, Etienne-Emile Baulieu

4 - Cytochrome P450 Enzymes in Brain
Pages 51-66
Margaret Warner, Adrian Wyss, Shigetaka Yoshida, Jan-Åke Gustafsson

5 - Methods for Estimating 11β-Hydroxysteroid Dehydrogenase Activity
Pages 67-76
Zygmunt Krozowski

6 - Steroid Hormone Binding to Intracellular Receptors: In Vitro and in Vivo Studies
Pages 79-95
Aernout D. van Haarst, Thomas F. Szuran

7 - Steroid Hormone Binding to Membrane Receptors
Pages 96-115
Miles Orchinik, Thomas F. Murray

8 - In Vitro Autoradiography for Steroid Receptors
Pages 116-142
Neil J. MacLusky, He Yuan, Debbie Bowlby, Richard B. Hochberg, Theodore J. Brown

9 - Immunocytochemical Studies on Glucocorticoid Receptor
Pages 143-161
Antonio Cintra, Gunnar Akner, Rafael Coveñas, Mercedes de León, Ann-Charlotte Wikström, Luigi F. Agnati, Jan-Åke Gustafsson, Kjell Fuxe

10 - Confocal Scanning Laser Microscopy of Steroid Receptors in Brain
Pages 162-174
Bas van Steensel, Erica P. van Binnendijk, Roel van Driel

11 - Ultrastructural Aspects of Steroid Receptor Localization: Immunocytochemical Perspective
Pages 175-188
Zsolt Liposits, Imre Kalló

12 - Hybridization Studies of Adrenocorticosteroid Receptors in the Central Nervous System
Pages 189-210
James P. Herman, Seung P. Kwak, Stanley J. Watson

13 - Detection and Characterization of Epalon Receptors: Novel Recognition Sites for Neuroactive Steroids That Modulate the GABAA Receptor Complex
Pages 211-225
Linda D. McCauley, Kelvin W. Gee

14 - Mutation Analysis of Steroid Hormone Receptors
Pages 226-241
Michael Karl, Heinrich M. Schulte, George P. Chrousos

15 - Protein–DNA-Binding Assay for Analysis of Steroid-Sensitive Neurons in Mammalian Brain
Pages 245-264
Yuan-shan Zhu, Donald W. Pfaff

16 - Gene Transfection Studies Using Recombinant Steroid Receptors
Pages 265-276
Klaus Damm

17 - Regulation of Neuropeptide Genes: Determination of Responsiveness to Steroids and Identification of Receptors in Brain Nuclei
Pages 277-295
Joke J. Cox, Sofia Lopes da Silva, Wiljan Hendriks, J. Peter H. Burbach

18 - Cloning of Steroid-Responsive mRNAs by Differential Hybridization
Pages 296-313
Nancy R. Nichols, Jeffrey N. Masters, Caleb E. Finch

19 - Molecular Correlates of Corticosterone Action in Hippocampal Subregions
Pages 314-329
Suresh M. Nair, James H. Eberwine

20 - Glucocorticoid-Determined Protein Synthesis
Pages 330-341
Linda A. Dokas

21 - Use of Antisense Oligodeoxynucleotides to Block Gene Expression in Central Nervous System
Pages 342-356
Margaret M. McCarthy

22 - Steroid Regulation of Neuronotrophic Activity: Primary Microcultures of Midbrain Raphe and Hippocampus
Pages 359-371
Efrain C. Azmitia, Xiao Ping Hou

23 - Central Glucocorticoid Receptors and Neuronal Plasticity
Pages 372-382
Kjell Fuxe, Antonio Cintra, Gerson Chadi, Jan-Åke Gustafsson, Luigi F. Agnati

24 - Steroid Action on Neuronal Structure
Pages 383-402
Catherine S. Woolley, Elizabeth Gould

25 - Electron Microscopic Double and Triple Labeling Immunocytochemistry in Elucidation of Synaptological Interactions between Ovarian Steroid-Sensitive Neurons and Circuits
Pages 403-416,416a,417-434
Csaba Leranth, Frederick Naftolin, Marya Shanabrough, Tamas L. Horvath

26 - Gene-Mediated Steroid Control of Neuronal Activity
Pages 435-445
Marian Joëls

27 - Electrophysiological Studies of Neurosteroid Modulation of γ-Aminobutyric Acid Type A Receptor
Pages 446-467
C. Hill-Venning, D. Belelli, J.A. Peters, J.J. Lambert

28 - In Vitro Approaches to Studying Glucocorticoid Effects on Gene Expression in Neurons and Glia
Pages 468-480
Martha Churchill Bohn

29 - Behavioral Approaches to Study Function of Corticosteroids in Brain
Pages 483-495
Melly Silvana Oitzl

30 - Adrenocorticosteroids and Cardiovascular Regulation: Methods for Surgery and Blood Pressure Measurements
Pages 496-509
Elise P. Gómez Sánchez

31 - Steroids and Central Regulation of Immune Response
Pages 510-522
Bernd Schöbitz

32 - Steroid Hormone Effects on Brain: Novel Insights Connecting Cellular and Molecular Features of Brain Cells to Behavior
Pages 525-542
Bruce S. McEwen

Index
Pages 543-552