Neural Stem Cells: Methods and Protocols

Front Matter ....Pages i-xiv
 Generation of Neural Stem Cells from Induced Pluripotent Stem Cells  (Marcel M. Daadi)....Pages 1-7
 Making NSC and Neurons from Patient-Derived Tissue Samples  (Odity Mukherjee, Shubhra Acharya, Mahendra Rao)....Pages 9-24
 Generation of Definitive Neural Progenitor Cells from Human Pluripotent Stem Cells for Transplantation into Spinal Cord Injury  (Mohamad Khazaei, Christopher S. Ahuja, Christopher E. Rodgers, Priscilla Chan, Michael G. Fehlings)....Pages 25-41
 Derivation of Neural Stem Cells from Human Parthenogenetic Stem Cells  (Rodolfo Gonzalez, Ibon Garitaonandia, Andrey Semechkin, Russell Kern)....Pages 43-57
 Chemically Defined Neural Conversion of Human Pluripotent Stem Cells  (Yu Chen, Carlos A. Tristan, Sunil K. Mallanna, Pinar Ormanoglu, Steven Titus, Anton Simeonov et al.)....Pages 59-72
 In Vitro Functional Characterization of Human Neurons and Astrocytes Using Calcium Imaging and Electrophysiology  (Marita Grønning Hansen, Daniel Tornero, Isaac Canals, Henrik Ahlenius, Zaal Kokaia)....Pages 73-88
 Differentiation of Neural Stem Cells Derived from Induced Pluripotent Stem Cells into Dopaminergic Neurons  (Marcel M. Daadi)....Pages 89-96
 Midbrain Dopaminergic Neurons Differentiated from Human-Induced Pluripotent Stem Cells  (Fabiano Araújo Tofoli, Ana Teresa Silva Semeano, Ágatha Oliveira-Giacomelli, Maria Carolina Bittencourt Gonçalves, Merari F. R. Ferrari, Lygia Veiga Pereira et al.)....Pages 97-118
 Generating Neural Stem Cells from iPSCs with Dopaminergic Neurons Reporter Gene  (Hyenjong Hong, Marcel M. Daadi)....Pages 119-128
 Single-Cell Library Preparation of iPSC-Derived Neural Stem Cells  (Jeffrey Kim, Marcel M. Daadi)....Pages 129-143
 Bioinformatics Analysis of Single-Cell RNA-Seq Raw Data from iPSC-Derived Neural Stem Cells  (Jeffrey Kim, Marcel M. Daadi)....Pages 145-159
 Assay for Assessing Mitochondrial Function in iPSC-Derived Neural Stem Cells and Dopaminergic Neurons  (Gourav Roy-Choudhury, Marcel M. Daadi)....Pages 161-173
 Reference Transcriptome for Deriving Marmoset Induced Pluripotent Stem Cells  (Guang Yang, Hyenjong Hong, April Torres, Kristen E. Malloy, Gourav Roy-Choudhury, Jeffrey Kim et al.)....Pages 175-186
 Optimization of Differentiation of Nonhuman Primate Pluripotent Cells Using a Combinatorial Approach  (Steven L. Farnsworth, Zhifang Qiu, Anuja Mishra, Peter J. Hornsby)....Pages 187-197
 Isolation and Differentiation of Self-Renewable Neural Stem Cells from Marmoset-Induced Pluripotent Stem Cells  (Hyenjong Hong, Gourav Roy-Choudhury, Jeffrey Kim, Marcel M. Daadi)....Pages 199-204
 Lentiviral Infection of Mouse Bone Marrow Cells for Hematopoietic Stem Cell Transplantation  (Cang Chen, Michael J. Guderyon, Guo Ge, Robert A. Clark, Senlin Li)....Pages 205-213
 Central and Peripheral Secondary Cell Death Processes after Transient Global Ischemia in Nonhuman Primate Cerebellum and Heart  (Jea-Young Lee, Roger Lin, Hung Nguyen, Eleonora Russo, M. Grant Liska, Trenton Lippert et al.)....Pages 215-225
 Method for Stimulation of Hippocampal Neurogenesis by Transient Microneedle Insertion  (Shijie Song, Xiaoyung Kong, Juan Sanchez-Ramos)....Pages 227-235
Back Matter ....Pages 237-239