Multidisciplinary Approaches to Cholinesterase Functions

Front Matter....Pages i-xvi
Impact of Recombinant DNA Technology and Protein Structure Determination on Past and Future Studies on Acetylcholinesterase....Pages 1-15
Biosynthesis of the Molecular Forms of Acetylcholinesterase....Pages 17-24
Binding of Asymmetric (A l2 ) Acetylcholinesterase to C 2 Muscle Cells and to Cho Mutants Defective in Glycosaminoglycan Synthesis....Pages 25-32
Subunit Assembly and Glycosylation of Mammalian Brain Acetylcholinsterase....Pages 33-38
Molecular Organization of Recombinant Human Acetylcholinesterase....Pages 39-47
Expression and Refolding of Functional Human Butyrylcholinesterase from E. Coli ....Pages 49-52
Part 1: Genetic Variant of Human Acetylcholinesterase Part 2: SV-40 Transformed Cell Lines, for Example COS-1, but Not Parental Untransformed Cell Lines, Express Butyrylcholinesterase (BChE)....Pages 53-59
Heterogeneity of the Silent Phenotype of Human Butyrylcholinesterase - Identification of Eight New Mutations....Pages 61-64
Nematode Acetylcholinesterases: Several Genes and Molecular Forms of Their Products....Pages 65-74
 Drosophila Acetylcholinesterase: Analysis of Structure and Sensitivity to Insecticides by In Vitro Mutagenesis and Expression....Pages 75-81
Altered Forms of Acetylcholinesterase in Insecticide-Resistant Houseflies (Musca Domestica) ....Pages 83-86
PCR Generated Homologous DNA Probes and Sequence for Acetylcholinesterase Genes in Insect Pests....Pages 87-90
Testicular Gene Amplification and Impaired BCHE Transcription Induced in Transgenic Mice by the Human BCHE Coding Sequence....Pages 91-94
Three Dimensional Structure of Acetylcholinesterase....Pages 95-107
Sequence Alignment of Esterases and Lipases Based on 3-D Structures of Two Members of This Family....Pages 109-116
Structural Analysis of Acetylcholinesterase Ammonium Binding Sites....Pages 117-120
Structural Investigations of the Acetylcholinesterase....Pages 121-130
Substrate-Selective Inhibition and Peripheral Site Labeling of Acetylcholinesterase by Platinum(Terpyridine)Chloride....Pages 131-139
Cryptic Catalysis and Cholinesterase Function....Pages 141-148
Influence of Ionic Composition of the Medium on Acetylcholinesterase Conformation....Pages 149-156
Molecular Dissection of Functional Domains in Human Cholinesterases Expressed in Microinjected Xenopus Oocytes....Pages 157-164
Acetylcholinesterase Catalysis - Protein Engineering Studies....Pages 165-175
Site-Directed Mutagenesis of Functional Residues in Torpedo Acetylcholinesterase....Pages 177-183
Structurally Important Residues in the Region Ser91 to Asn98 of Torpedo Acetylcholinesterase....Pages 185-188
A Model of Butyrylcholinesterase Based on the X-Ray Structure of Acetylcholinesterase Indicates Differences in Specificity....Pages 189-194
Molecular Models for Human AChE and its Phosphonylation Products....Pages 195-199
Relationships Between Activity of Organophosphorus Inhibitors of Acetylcholinesterase and Accessibility of Phosphorus Atom as Estimated by Molecular Mechanics Calculations....Pages 201-207
Factors Influencing Acetylcholinesterase Regulation in Slow and Fast Skeletal Muscles....Pages 209-216
Compartmentalization of Acetylcholinesterase mRNA and Protein Expression in Skeletal Muscle in Vitro and in Vivo : Implications for Regulation at the Neuromuscular Junction....Pages 217-222
Towards a Functional Analysis of Cholinesterases in Neuro-Genesis: Histological, Molecular, and Regulatory Features of BCHE from Chicken Brain....Pages 223-231
Acetylcholinesterase as a Modulatory Neuroprotein and its Influence on Motor Control....Pages 233-242
Different Influence of Inhibitors on Acetylcholinesterase Molecular forms G1 and G4 Isolated from Alzheimer’s Disease and Control Brains....Pages 243-249
Acetylcholinesterase Inhibition by Novel Carbamates: A Kinetic and Nuclear Magnetic Resonance Study....Pages 251-259
Experimental Acetylcholinesterase Autoimmunity....Pages 261-268
Clinical Implications of Cholinesterase Aberrations in Syndromes of Hemopoietic Cell Division....Pages 269-276
Acetylcholinesterase: A Pretreatment Drug for Organophosphate Toxicity....Pages 277-284
Recommendations for Nomenclature in Cholinesterases....Pages 285-288
Back Matter....Pages 289-293