Motility Assays for Motor Proteins

Content: 
Series Editors
Page ii

Edited by
Page iii

Copyright page
Page iv

Contributors
Pages xi-xiv

Preface
Page xv
Jonathan M. Scholey

Chapter 1 In Vitro Methods for Measuring Force and Velocity of the Actin-Myosin Interaction Using Purified Proteins Original Research Article
Pages 1-21
Hans M. Warrick, Robert M. Simmons, Jeffrey T. Finer, Taro Q.P. Uyeda, Steven Chu, James A. Spudich

Chapter 2 Myosin-Specific Adaptations of the Motility Assay Original Research Article
Pages 23-49
James R. Sellers, Giovanni Cuda, Fei Wang, Earl Homsher

Chapter 3 Motility of Myosin I on Planar Lipid Surfaces Original Research Article
Pages 51-63
Henry G. Zot, Thomas D. Pollard

Chapter 4 Microtubule and Axoneme Gliding Assays for Force Production by Microtubule Motor Proteins Original Research Article
Pages 65-74
Bryce M. Paschal, Richard B. Vallee

Chapter 5 Analyzing Microtubule Motors in Real Time Original Research Article
Pages 75-88
S.A. Cohn, W.M. Saxton, R.J. Lye, J.M. Scholey

Chapter 6 Assays of Axonemal Dynein-Driven Motility Original Research Article
Pages 89-104
Winfield S. Sale, Laura A. Fox, Elizabeth F. Smith

Chapter 7 Preparation of Marked Microtubules for the Assay of the Polarity of Microtubule-Based Motors by Fluorescence Microscopy Original Research Article
Pages 105-113
Jonathon Howard, Anthony A. Hyman

Chapter 8 Expression of Microtubule Motor Proteins in Bacteria for Characterization in in Vitro Motility Assays Original Research Article
Pages 115-127
Rashmi Chandra, Sharyn A. Endow

Chapter 9 Tracking Nanometer Movements of Single Motor Molecules Original Research Article
Pages 129-136
Michael P. Sheetz, Scot C. Kuo

Chapter 10 Assay of Microtubule Movement Driven by Single Kinesin Molecules Original Research Article
Pages 137-147
Jonathon Howard, Alan J. Hunt, Sung Baek

Chapter 11 In Vitro Motility Assays Using Microtubules Tethered to Tetrahymena Pellicles Original Research Article
Pages 149-165
Vivian A. Lombillo, Martine Coue, J. Richard McIntosh

Chapter 12 Use of ATP Analogs in Motor Assays Original Research Article
Pages 167-177
Takashi Shimizu, Yoko Y. Toyoshima, Ronald D. Vale

Chapter 13 Myosin-Mediated Vesicular Transport in the Extruded Cytoplasm of Characean Algae Cells Original Research Article
Pages 179-190
Bechara Kachar, Raul Urrutia, Marcelo N. Rivolta, Mark A. McNiven

Chapter 14 Assay of Vesicle Motility in Squid Axoplasm Original Research Article
Pages 191-202
Scott T. Brady, Bruce W. Richards, Philip L. Leopold

Chapter 15 Assay of Membrane Motility in Interphase and MetaphaseXenopusEXTRACTS Original Research Article
Pages 203-226
Viki J. Allan

Chapter 16 Microtubule Motor-Dependent Formation of Tubulovesicular Networks from Endoplasmic Reticulum and Golgi Membranes Original Research Article
Pages 227-236
James M. McIlvain, Carilee Lamb Jr., Sandra Dabora, Michael P. Sheetz

Chapter 17 Cytoplasmic Extracts from the Eggs of Sea Urchins and Clams for the Study of Microtubule-Associated Motility and Bundling Original Research Article
Pages 237-251
Neal R. Gliksman, Stephen F. Parsons, E.D. Salmon

Chapter 18 Kinesin-Mediated Vesicular Transport in a Biochemically Defined Assay Original Research Article
Pages 253-266
Raul Urrutia, Douglas B. Murphy, Bechara Kachar, Mark A. McNiven

Chapter 19 An Assay for the Activity of Microtubule-Based Motors on the Kinetochores of Isolated Chinese Hamster Ovary Chromosomes Original Research Article
Pages 267-276
A.A. Hyman, T.J. Mitchison

Chapter 20 The Diatom Central Spindle as a Model System for Studying Antiparallel Microtubule Interactions during Spindle Elongation in Vitro Original Research Article
Pages 277-292
Christopher J. Hogan, Patrick J. Neale, Manlin Lee, W. Zacheus Cande

Index
Pages 293-299

Volumes in Series
Pages 301-304