Molecular Motors and the Cytoskeleton

Content: 
Contributors to volume 196
Pages ix-xii

Preface
Pages xiii-xiv
Richard B. Vallee

Methods in enzymology
Pages xv-xxx

[1] Rapid, high-yield purification of intestinal brush border myosin I Original Research Article
Pages 3-11
Helena Swanljung-Collins, Jimmy H. Collins

[2] Purification of myosin I and myosin I heavy chain kinase from Acanthamoeba castellanii Original Research Article
Pages 12-23
Thomas J. Lynch, Hanna Brzeska, Ivan C. Baines, Edward D. Korn

[3] Purification and characterization of myosin II heavy chain kinase A from Dictyostelium Original Research Article
Pages 23-34
Quintus G. Medley, Sheu-Fen Lee, Graham P. CГґtГ©

[4] Purification and properties of myosin light chain kinases Original Research Article
Pages 34-47
Mary Anne Conti, Robert S. Adelstein

[5] F-actin affinity chromatography of detergent-solubilized plasma membranes: Purification and initial characterization of ponticulin from Dictyostelium discoideum Original Research Article
Pages 47-65
Linda J. Wuestehube, David W. Speicher, Aneesa Shariff, Elizabeth J. Luna

[6] Purification of calpactins I and II and isolation of N-terminal tail of calpactin I Original Research Article
Pages 65-69
John R. Glenney Jr.

[7] Isolation of actin-binding proteins from Dictyostelium discoideum Original Research Article
Pages 70-83
A.R. Bresnick, J. Condeelis

[8] Preparation of 30,000-Da actin cross-linking protein from Dictyostelium discoideum Original Research Article
Pages 84-91
Marcus Fechheimer, Ruth Furukawa

[9] Polyproline affinity method for purification of platelet profilin and modification with pyrene-maleimide Original Research Article
Pages 92-99
Paul A. Janmey

[10] Affinity chromatography-based purification of profilin: Actin Original Research Article
Pages 100-118
Michael Rozycki, Clarence E. Schutt, Uno Lindberg

[11] Purification of actobindin from Acanthamoeba castellanii Original Research Article
Pages 119-125
Michael R. Bubb, Edward D. Korn

[12] Purification and characterization of low-molecular-weight actin-depolymerizing proteins from brain and cultured cells Original Research Article
Pages 125-140
James R. Bamburg, Laurie S. Minamide, Todd E. Morgan, Steven M. Hayden, Kenneth A. Giuliano, Anna Koffer

[13] Purification of cap Z from chicken skeletal muscle Original Research Article
Pages 140-154
James F. Casella, John A. Cooper

[14] Purification of kinesin from bovine brain and assay of microtubule-stimulated ATPase activity Original Research Article
Pages 157-175
Mark C. Wagner, K.Kevin Pfister, Scott T. Brady, George S. Bloom

[15] Isolation of kinesin using initial batch ion exchange Original Research Article
Pages 175-181
David D. Hackney

[16] Purification of brain cytoplasmic dynein and characterization of its in Vitro properties Original Research Article
Pages 181-191
Bryce M. Paschal, Howard S. Shpetner, Richard B. Vallee

[17] Purification and characterization of dynamin Original Research Article
Pages 192-201
Howard S. Shpetner, Richard B. Vallee

[18] Purification and characterization of Salmo gairdneri outer arm dynein Original Research Article
Pages 201-222
Anthony G. Moss, Jean-Luc Gatti, Stephen M. King, George B. Witman

[19] Purification and characterization of dynein from pig tracheal cilia Original Research Article
Pages 223-234
Annette T. Hastie

[20] Purification of tubulin and tau from chicken erythrocytes: Tubulin isotypes and mechanisms of microtubule assembly Original Research Article
Pages 235-246
Douglas B. Murphy

[21] Reversible assembly purification of taxol-treated microtubules Original Research Article
Pages 246-253
Christine A. Collins

[22] Preparation and functional assay of pure populations of tyrosinated and detyrosinated tubulin Original Research Article
Pages 254-264
Steven J. Chapin, Jeannette ChloГ« Bulinski

[23] Detection of acetylated О±-tubulin by specific antibodies Original Research Article
Pages 264-274
Michel LeDizet, Gianni Piperno

[24] Isolation of microtubules, adligin, and other microtubule-associated proteins from Caenorhabditis elegans Original Research Article
Pages 274-285
Eric Aamodt

[25] Microtubules, tubulin, and microtubule-associated proteins of trypanosomes Original Research Article
Pages 285-299
Derrick Robinson, Pauline Beattie, Trevor Sherwin, Keith Gull

[26] Use of actin filament and microtubule affinity chromatography to identify proteins that bind to the cytoskeleton Original Research Article
Pages 303-319
Kathryn G. Miller, Christine M. Field, Bruce M. Alberts, Douglas R. Kellogg

[27] Molecular genetic tools for study of the cytoskeleton in Dictyostelium Original Research Article
Pages 319-334
Thomas T. Egelhoff, Margaret A. Titus, Dietmar J. Manstein, Kathleen M. Ruppel, James A. Spudich

[28] Screening for Dictyostelium mutants defective in cytoskeletal proteins by colony immunoblotting Original Research Article
Pages 334-348
E. Wallraff, G. Gerisch

[29] Selection of Chlamydomonas dynein mutants Original Research Article
Pages 348-355
Ritsu Kamiya

[30] Cloning and analyzing genes encoding cytoskeletal proteins in yeast Saccharomyces cerevisiae Original Research Article
Pages 355-368
Tim C. Huffaker, Anthony P. Bretscher

[31] Expression of myosin and actin in Escherichia coli Original Research Article
Pages 368-389
Elizabeth McNally, Regina Sohn, Stewart Frankel, Leslie Leinwand

[32] Synthesis of cDNAs from synthetic oligonucleotides using troponin C as an example Original Research Article
Pages 389-396
Gong-Qiao Xu, Sarah E. Hitchcock-DeGregori

[33] Assays for actin sliding movement over myosin-coated surfaces Original Research Article
Pages 399-416
Stephen J. Kron, Yoko Y. Toyoshima, Taro Q.P. Uyeda, James A. Spudich

[34] Demembranation and reactivation of mammalian spermatozoa from golden hamster and ram Original Research Article
Pages 417-428
Sumio Ishijima, George B. Witman

[35] Photocatalytic cleavage of proteins with vanadate and other transition metal complexes Original Research Article
Pages 428-442
I.R. Gibbons, Gabor Mocz

[36] Vanadate-mediated photocleavage of myosin Original Research Article
Pages 442-449
Christine R. Cremo, Jean C. Grammer, Ralph G. Yount

[37] Strategies and reagents for photoaffinity labeling of mechanochemical proteins Original Research Article
Pages 449-466
Stephen M. King, Hyuntae Kim, Boyd E. Haley

[38] Microtubule polarity determination based on formation of protofilament hooks Original Research Article
Pages 469-477
Steven R. Heidemann

[39] Preparation of modified tubulins Original Research Article
Pages 478-485
Anthony Hyman, David Drechsel, Doug Kellogg, Steve Salser, Ken Sawin, Pam Steffen, Linda Wordeman, Tim Mitchison

[40] Measurement of actin polymerization and cross-linking in agonist-stimulated cells Original Research Article
Pages 486-496
J. Condeelis, A.L. Hall

[41] Preparation and characterization of tetramethylrhodamine-labeled myosin Original Research Article
Pages 497-505
Yu-Li Wang

[42] Analyzing dynamic properties of intermediate filaments Original Research Article
Pages 506-525
Karen L. Vikstrom, Rita K. Miller, Robert D. Goldman

Author index
Pages 527-544

Subject index
Pages 545-559