Molecular microbial diagnostic methods : pathways to implementation for the food and water industries

Content: Title page
 Table of Contents
 Copyright
 Contributors
 Preface
 Chapter 1: Food industry current status
 Abstract
 Introduction
 Molecular methods currently used
 Laboratory automation in molecular methods
 Challenges
 Acceptance of use by the food industry
 Compliance with current legal requirements
 Disclaimer
 Chapter 2: Future directions for molecular microbial diagnostic methods for the food industry
 Abstract
 Introduction
 Evolution of food microbiology diagnostics: from petri dishes to PCR
 Why introduce an alternative molecular diagnostic method? Characteristics of an ideal food molecular methodPast and current challenges
 Current challenges
 Concluding remarks
 Chapter 3: Current status of molecular microbiological techniques for the analysis of drinking water
 Abstract
 Introduction and overview
 The current state of play
 The influence of standard and reference methods
 Molecular techniques for testing potable water quality
 Application of molecular techniques to wastewater
 Conclusions
 Chapter 4: What is now required for water?
 Abstract
 Introduction and overview
 Indicator organisms
 Pathogens in drinking water Total heterotrophic bacteriaFeasibility of the use of routine rapid water molecular methods
 Future predictions
 Conclusions
 Chapter 5: CEN/ISO standards for both culture and molecular methods
 Abstract
 Introduction
 Standards, standardization bodies, and structures in the microbiology of the food chain
 Standards developed in the microbiology of the food chain
 Status of novel technologies
 Conclusions
 Chapter 6: Laboratory validation, verification, and accreditation of molecular methods
 Abstract
 Alternative methods
 Foundations of an accredited laboratory
 Quality systems Cornerstones of accreditationMaintaining accreditation
 Customer education
 Health and safety
 Chapter 7: DNA extraction: finding the most suitable method
 Abstract
 Boiling method
 Column extraction
 Magnetic beads
 FTA™ cards
 RNA extraction methods
 Conclusions
 Chapter 8: Assessing organism viability and interpreting genomic unit versus colony forming unit data for water and food borne microorganisms, such as Legionella, Campylobacter, Salmonella, and Listeria
 Abstract
 Introduction
 Polymerase chain reaction
 Assessing microbial viability
 Overcoming the viability hurdle When to use viability discriminationStandardization and quality assurance
 Proficiency testing
 Conclusions
 Chapter 9: MALDI-TOF MS: a rapid microbiological confirmation technique for food and water analysis
 Abstract
 Introduction and overview
 Current confirmation methods and advantages of MALDI-TOF MS
 MALDI-TOF MS Listeria speciation validation
 Chapter 10: Chapter highlights, future requirements, and conclusions
 Chapter highlights
 Future requirements and conclusions
 Index