Mitochondria

Content: 
Contributors
Pages xiii-xvi

Preface
Page xvii
Liza A. Pon, Eric A. Schon

Chapter 1 Isolation and subfractionation of mitochondria from animal cells and tissue culture lines Review Article
Pages 1-35
Francesco Pallotti, Giorgio Lenaz

Chapter 2 Isolation and subfractionation of mitochondria from the yeast Saccharomyces cerevisiae Review Article
Pages 37-51
Kerstin Diekert, Anton I.P.M. de Kroon, Gyula Kispal, Roland Lill

Chapter 3 Isolation and subfractionation of mitochondria from plants Review Article
Pages 53-74
A.H. Millar, A. Liddell, C.J. Leaver

Chapter 4 Assessing functional integrity of mitochondria in vitro and in vivo Review Article
Pages 75-96
Mauro Degli Esposti

Chapter 5 Assaying mitochondrial respiratory complex activity in mitochondria isolated from human cells and tissues Review Article
Pages 97-117
Mark A. Birch-Machin, Douglass M. Turnbull

In vivo measurements of respiration control by cytochrome c oxidase and in situ analysis of oxidative phosphorylation Review Article
Pages 119-131
Gaetano Villani, Giuseppe Attardi

Assay of mitochondrial ATP synthesis in animal cells Review Article
Pages 133-145
Giovanni Manfredi, Antonella Spinazzola, Nicoletta Checcarelli, Ali Naini

Chapter 8 Measurement of membrane permeability and permeability transition of mitochondria Review Article
Pages 147-158
Naoufal Zamzami, Carine Maisse, Didier Métivier, Guido Kroemer

Chapter 9 Assaying actin-binding activity of mitochondria in yeast Review Article
Pages 159-173
Istvan R. Boldogh, Liza A. Pon

Chapter 10 Analysis and prediction of mitochondrial targeting peptides Review Article
Pages 175-187
Olof Emanuelsson, Gunnar von Heijne, Gisbert Schneider

Chapter 11 Assaying protein import into mitochondria Review Article
Pages 189-215
Michael T. Ryan, Wolfgang Voos, Nikolaus Pfanner

Analysis of protein-protein interactions in mitochondria by coimmunoprecipitation and chemical cross-linking Review Article
Pages 217-230
Johannes M. Herrmann, Benedikt Westermann, Walter Neupert

Chapter 13 Blue-native gels to isolate protein complexes from mitochondria Review Article
Pages 231-244
Hermann Schägger

Chapter 14 Application of electron tomography to mitochondrial research Review Article
Pages 245-256
Carmen A. Mannella

Chapter 15 Epitope tagging and visualization of nuclear-encoded mitochondrial proteins in yeast Review Article
Pages 257-276
Dan W. Nowakowski, Theresa C. Swayne, Liza A. Pon

Chapter 16 Targeting of green fluorescent protein to mitochondria Review Article
Pages 277-283
Koji Okamoto, Philip S. Perlman, Ronald A. Butow

Assessment of mitochondrial membrane potential in situ using single potentiometric dyes and a novel fluorescence resonance energy transfer technique Review Article
Pages 285-309
James A. Dykens, Amy K. Stout

Chapter 18 Optical imaging techniques (histochemical, immunohistochemical, and in situ hybridization staining methods) to visualize mitochondria Review Article
Pages 311-332
Kurenai Tanji, Eduardo Bonilla

Chapter 19 Visualization of mitochondrial movement in yeast Review Article
Pages 333-351
Hyeong-Cheol Yang, Viviana Simon, Theresa C. Swayne, Liza Pon

Targeting of reporter molecules to mitochondria to measure calcium, ATP, and pH Review Article
Pages 353-380
Anna M. Porcelli, Paolo Pinton, Edward K. Ainscow, Anna Chiesa, Michela Rugolo, Guy A. Rutter, Rosario Rizzuto

Genetic transformation of Saccharomyces cerevisiae mitochondria Review Article
Pages 381-396
Nathalie Bonnefoy, Thomas D. Fox

Transmitochondrial technology in animal cells Review Article
Pages 397-412
Carlos T. Moraes, Runu Dey, Antoni Barrientos

Diagnostic assays for defects in mitochondrial DNA replication and transcription in yeast and human cells Review Article
Pages 413-427
Bonnie L. Seidel-Rogol, Gerald S. Shadel

Analysis of mitochondrial translation products in Vivo and in organello in yeast Review Article
Pages 429-438
Benedikt Westermann, Johannes M. Herrmann, Walter Neupert

Numerical methods for handling uncertainty in microarray data: An example analyzing perturbed mitochondrial function in yeast Review Article
Pages 439-495
Charles B. Epstein, Walker Hale IV, Ronald A. Butow

Index
Pages 497-511

Volumes in series
Pages 513-519