Microinjection: Methods and Protocols

Front Matter ....Pages i-xiv
 Microinjection and Micromanipulation: A Historical Perspective  (Wenhao Xu)....Pages 1-16
 Production of Transgenic Mice by Pronuclear Microinjection  (Xin-an Pu, Anthony P. Young, H. Michael Kubisch)....Pages 17-41
 Transgene Recombineering in Bacterial Artificial Chromosomes  (Michael G. Zeidler, Thomas L. Saunders)....Pages 43-69
 Using TARGATT™ Technology to Generate Site-Specific Transgenic Mice  (Ruby Yanru Chen-Tsai)....Pages 71-86
 Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method  (Takuya Sato, Takehiko Ogawa)....Pages 87-98
 Chimeric Mouse Generation by ES Cell Blastocyst Microinjection and Uterine Transfer  (Yubin Du, Wen Xie, Fan Zhang, Chengyu Liu)....Pages 99-114
 Creating Knockin Alleles in Mouse Embryonic Stem Cells by CRISPR/Cas9-Mediated Homologous Recombination Without Drug Selection  (Pinghu Liu, Yan Li, Jingqi Lei, Lijin Dong)....Pages 115-137
 Using CRISPR/Cas9 for Gene Knockout in Immunodeficient NSG Mice  (Yubin Du, Wen Xie, Fan Zhang, Uimook Choi, Chengyu Liu, Colin L. Sweeney)....Pages 139-168
 Generation of CRISPR-Edited Rodents Using a Piezo-Driven Zygote Injection Technique  (Melissa A. Scott, Yueh-Chiang Hu)....Pages 169-178
 Delivery of CRISPR-Cas9 into Mouse Zygotes by Electroporation  (Wenning Qin, Haoyi Wang)....Pages 179-190
 Generation of Conditional Knockout Mice by Sequential Insertion of Two loxP Sites In Cis Using CRISPR/Cas9 and Single-Stranded DNA Oligonucleotides  (Ye Liu, Yubin Du, Wen Xie, Fan Zhang, Douglas Forrest, Chengyu Liu)....Pages 191-210
 Improvement of Mouse Cloning from Any Type of Cell by Nuclear Injection  (Sayaka Wakayama, Satoshi Kishigami, Teruhiko Wakayama)....Pages 211-228
 The CARD Method for Simple Vitrification of Mouse Oocytes: Advantages and Applications  (Naomi Nakagata, Jorge Sztein, Toru Takeo)....Pages 229-242
 The CARD Method for Mouse Sperm Cryopreservation and In Vitro Fertilization Using Frozen-Thawed Sperm  (Toru Takeo, Jorge Sztein, Naomi Nakagata)....Pages 243-256
 Isolation and Analysis of a Genome-Edited Single-Hepatocyte from a Cas9 Transgenic Mouse Line  (Takayuki Sakurai, Akiko Kamiyoshi, Masato Ohtsuka, Channabasavaiah B. Gurumurthy, Masahiro Sato, Takayuki Shindo)....Pages 257-271
 Microinjection and Oviduct Transfer Procedures for Rat Model Generation with CRISPR-Cas9 Technology  (Tuija M. Alcantar, Xin Y. Rairdan)....Pages 273-294
 Molecular Aspects of Zinc Finger Nucleases (ZFNs)-Mediated Gene Editing in Rat Embryos  (Xiaoxia Cui)....Pages 295-312
 Organ Generation from Knockedout Rat Blastocysts Complemented with Pluripotent Stem Cells  (Masumi Hirabayashi, Shinichi Hochi)....Pages 313-326
 Generation of Rabbit Models by Gene Editing Nucleases  (Dongshan Yang, Jie Xu, Y. Eugene Chen)....Pages 327-345
 Production of Genetically Engineered Porcine Embryos by Handmade Cloning  (Rong Li, Jinxin Miao, Zhongde Wang)....Pages 347-360
 Electrofusion of 2-Cell Embryos for Porcine Tetraploid Embryo Production  (Qingran Kong, Zhonghua Liu)....Pages 361-371
 Gene Knockouts in Goats Using CRISPR/Cas9 System and Somatic Cell Nuclear Transfer  (Zhiqiang Fan, Min Yang, Misha Regouski, Irina A. Polejaeva)....Pages 373-390
 Generating Goat Mammary Gland Bioreactors for Producing Recombinant Proteins by Gene Targeting  (Liyou An, Lan Yang, Yuejin Huang, Yong Cheng, Fuliang Du)....Pages 391-401
 Production of Transgenic Chickens Using Cultured Primordial Germ Cells and Gonocytes  (Ellen J. Collarini, Philip A. Leighton, Marie-Cecile Van de Lavoir)....Pages 403-430
 Using Microinjection to Generate Genetically Modified Caenorhabditis elegans by CRISPR/Cas9 Editing  (Jyoti Iyer, Nicole DeVaul, Tyler Hansen, Ben Nebenfuehr)....Pages 431-457
 Microinjection in Zebrafish for Genome Editing and Functional Studies  (Wuhong Pei, Shawn M. Burgess)....Pages 459-474
 Microinjection of Marine Fish Eggs  (Rie Goto, Taiju Saito, Takahiro Matsubara, Etsuro Yamaha)....Pages 475-487
 Generating Gene Knockout Oryzias latipes and Rice Field Eel Using TALENs Method  (Daji Luo, Ke Feng, Zuoyan Zhu, Wei Hu)....Pages 489-506
 Functional Studies of Transcriptional Cofactors via Microinjection-Mediated Gene Editing in Xenopus  (Yuki Shibata, Lingyu Bao, Liezhen Fu, Bingyin Shi, Yun-Bo Shi)....Pages 507-524
 Microinjection of Live Mammalian Cells: A Delivery Method that Provides Added Versatility to the Study of Cellular Function  (Robert F. Gahl)....Pages 525-536
Back Matter ....Pages 537-540