Microfluidic Methods for Molecular Biology

Front Matter....Pages i-xii
Microfluidic Platforms for Quantitative Biology Studies in Model Organisms....Pages 1-18
Microfluidic Methods in Single Cell Biology....Pages 19-54
Convective PCR Thermocycling with Smartphone-Based Detection: A Versatile Platform for Rapid, Inexpensive, and Robust Mobile Diagnostics....Pages 55-69
Forensic Typing of Single Cells Using Droplet Microfluidics....Pages 71-94
Microfluidic Approaches to Fluorescence In Situ Hybridization (FISH) for Detecting RNA Targets in Single Cells....Pages 95-112
Microfluidic Multistage Integration for Analysis of Circulating Exosomes....Pages 113-139
Microfluidic Single-Cell Functional Proteomics....Pages 141-159
Microfluidics for DNA and Protein Analysis with Multiplex Microbead-Based Assays....Pages 161-187
Single-Cell Phenotypic Screening in Inverse Metabolic Engineering....Pages 189-204
Microfluidic Paper-Based Multiplexing Biosensors for Electrochemical Detection of Metabolic Biomarkers....Pages 205-218
Droplet Microfluidics for Screening of Surface-Marker and Secretory Protein Expression....Pages 219-233
Wire-guided Droplet Manipulation for Molecular Biology....Pages 235-252
Mechanical and Electrical Principles for Separation of Rare Cells....Pages 253-294
Microfluidics for High-Throughput Cellular Isolation and Analysis in Biomedicine....Pages 295-322
Microfluidics for Cell Culture....Pages 323-347
Microfluidic Chromatin Immunoprecipitation for Analysis of Epigenomic Regulations....Pages 349-363
Back Matter....Pages 365-376