Microbiology Experiments: A Health Science Perspective

Preface......Page 1
Laboratory Safety......Page 2
Laboratory Safety Agreement......Page 3
.. Microbiology......Page 4
1. Ubiquity of Microorganisms......Page 6
2. Bright-field Light Microscopy, Including History & Working Principles......Page 12
3. Microscopic (Bright-field & Dark.field) Determination of Cell Motility......Page 24
.. Microorganisms......Page 32
4. Simple Stains: Positive and Negative Stains......Page 34
5. Multiple and Differential Stains......Page 40
.. Microbial Growth......Page 52
6. Pure Culture and Aseptic Technique......Page 54
7. Defined, Undefined, Selective, and Differential Media......Page 60
8. Quantification of Microorganisms......Page 64
.. Environment & Microbial Growth......Page 72
9. Aerobic and Anaerobic Growth......Page 74
10. The Effect of Incubation Temperature on Generation Time......Page 78
.. Control of Microbial Growth......Page 86
11. Moist and Dry Heat Sterilization: Thermal Death Point & Thermal Death Time......Page 88
12. Control of Microbial Growth with Ultraviolet Light......Page 98
13. Osmotic Pressure, and Its Effect on the Rate and Amount of Microbial Growth......Page 104
14. Antiseptics and Antibiotics......Page 112
.. Microbial Genetics......Page 122
15. Selection of Bacterial Mutants Resistant to Antibiotics......Page 124
16. Transformation: A Form of Genetic Recombination......Page 130
17. Bacterial Conjugation......Page 136
18. Gene Regulation: Induction and Catabolite Repression......Page 144
Introduction......Page 150
19. Microscopic Identification of Fungi......Page 154
20. Parasitology: Protozoa and Helminths......Page 172
21. Prokaryotic Viruses: Bacteriophage Isolation and Titering......Page 186
Introduction to Medical Microbiology......Page 194
22. Normal Skin Flora......Page 196
23. Respiratory Microorganisms......Page 202
24. Identification of Enteric Gram-Negative Rods......Page 208
25. Clinical Unknown Identification......Page 214
Introduction to Some Immunological Principles and Techniques......Page 222
26. Differential White Blood Cell Stains......Page 224
27. Lysozyme, and Enzymatic Form of Natural Resistance......Page 230
28. Trad’tl Agglutination Reactions Employing Microbial & Nonmicrobial Antigens......Page 236
29. Lancefield Grouping Pathogenic Streptococci.Latex Slide Agglutination Test......Page 248
30. Use of Enzyme.Linked Immunosorbent Assay Test for Coccidioides immitis ID......Page 256
31. An Ouchterlony Double Immunodiffusion Test for Coccidioides immitis ID......Page 264
Introduction to the Prevention and Control of Communicable Diseases......Page 274
32. Epidemiology: A Staphylococcus Carrier Study......Page 276
33. Bacteriological Exam of Water: Multiple-Tube Fermentation & Membrane Filter......Page 288
Introduction to Biotechnology......Page 302
34. Identifying DNA with Restriction Enzymes......Page 304
35. Identification of Bacteria Using the Ribosomal Data Project......Page 310
Introduction to the Individual Projects......Page 316
36. Hydrocarbon-Degrading Bacteria, Cleaning Up After Oil Spills......Page 318
37. Luminescent Bacteria: Bacteria That Produce Light......Page 320
38. Methylotrophs, Organisms That Grow on One-Carbon Compounds......Page 322
39. Deinococcus, Bacteria with Out-Of-This-World Capabilities......Page 324
App1: Living Microorganisms Chosen for Study in this Manual......Page 326
App2: Dilution Practice Problems......Page 327
App3: Metric System, Use with Conversions to English System of Measurement......Page 329
App4: pH Adjustment of Liquid and Agar Growth Media......Page 330
App5: Use of Ocular Micrometer.Measure of Relative & Absolute Cell Size......Page 332
App6: Use of Hemocytometer for Determining Total Cell No. in Liquid Susp.......Page 334
App7: Preparation of Covered Slide Cultures for Study of Intact Structure......Page 336
Index......Page 338
Color Plates......Page 344