Micro Total Analysis Systems 2002: Proceedings of the µTAS 2002 Symposium, held in Nara, Japan, 3–7 November 2002 Volume 1

Front Matter....Pages i-xxxix
Airborn Chemistry Levitated Protein Droplets as a Novel Analytical Tool for Nucleation Screening in Macromolecular Crystallisation....Pages 54-57
On-Line Monitoring of Airborne Chemistry in Levitated Droplets: In-Situ Synthesis and Application of SERS Active Ag-Sols for Trace Analysis by Raman Spectrosmetry....Pages 58-60
Chip-to-World Interfaces for High-Throughput Lab-on-a-Chip Devices....Pages 61-63
Electrodeless Dielectrophoretic Trapping and Separation of Cells....Pages 25-27
SiO 2 Nozzle Array-Based Patch-Clamp Microsystem....Pages 28-30
A Programmable Cell Assay Platform for Kinetic Studies of a Single Cell....Pages 31-33
An Integrated Microdialysis-Based System....Pages 34-36
User-Assembly, Fully Integrated Micro Chemical Laboratory Using Biochemical IC Chips for Wearable/Implantable Applications....Pages 37-39
Microfluidic Reactor Array for Multistep Droplet Reactions....Pages 40-42
Single Molecular Detection of DNA/Protein and Its Application to Biochips....Pages 43-44
Exploring FET Concepts for Lab-on-a-Chip....Pages 1-6
Monitoring of Cell Growth, Oxygen and pH in Microfermentors....Pages 7-9
Observation of Response to Stimuli in Oscillating Cells Patterned by Microfabricated Structure....Pages 10-12
On-Chip Agarose Microchamber (AMC) Array Cell-Cultivation System for Topographical Control of Neural Network....Pages 13-15
DNA Separation in Gradient of Confined Nanospace Generated by Nanoparticles in a Microchannel....Pages 45-47
Self-Assembled Magnetic Colloids for DNA Separations in Microfluidic Devices....Pages 48-50
A Microfabricated Device for Separating ∼200 Kilo-Base-Pair DNA Molecules in ∼15 Seconds....Pages 51-53
Integrated Size Exclusion and Reversed-Phase Electrochromatography....Pages 16-18
Dispersion Analysis of a Solid-Phase Microextraction Chip by Computational Fluid Dynamics....Pages 19-21
Interfacing Microchip CE with ICPMS for Element Speciation....Pages 22-24
The Mems Modeling System by Collaboration of Multi-Scale Simulators and Application to the Microreactor....Pages 64-66
FEM study of Coulter Counter with Water-Based Adaptable Aperture....Pages 67-69
Micromanipulating Magnetic Particles in Microfluidic Systems....Pages 70-72
Optimal Design of the Micromixer using Nonhomogeneous Multilayer Laminar Flow....Pages 73-75
Computational Simulations of Fluid Flow Dynamics, and Bead Packing in Solid Phase Extraction Microsystems....Pages 76-78
The Low-Voltage Cascade EOF PUMP: Comparing Theory with Published Data....Pages 79-81
Universal Joule Heating Model in Electrophoretic Separation Microchips....Pages 82-84
Mathematical Model of Interfacial Layer in Ultra-Fine Liquid Drop Based on Molecular Dynamics Simulation....Pages 85-87
Development of Micromixer Based on Instability of Interface Between Two Immiscible Liquids....Pages 88-90
Theoretical Study of AC Electroosmotic Flows in Non-Uniformly Charged Microchannels....Pages 91-93
Numerical Simulation of Mixing in a Micro-Channel with Non-Uniform Zeta Potential Surface....Pages 94-96
Injection of Sample Bands from open Channels Into Packed Separation Columns....Pages 97-99
Microscale Measurements of Flow Bounded by Air-Water Interfaces....Pages 100-102
Virtual Flow Channel: A Novel Micro-fluidics System with Orthogonal, Dynamic Control of Sample Flow Dimensions....Pages 103-105
Microfabrication of 3D Oblique Structures by Inclined UV Lithography....Pages 106-108
A New Fabrication Technique of a DNA Electrophoresischip by Silicon Micromachining Technology....Pages 109-111
Precision Patterning of PDMS Thin Films: A New Fabrication Method and Its Applications....Pages 112-114
Fabrication of Microfluidic Channels with Symmetric Cross-Sections for Integrated NMR Analysis....Pages 115-117
Precise and Feasible Fabrication Process of Microfluidic Devices on a Borosilicate Glass Chip....Pages 118-120
Laminated Electrodes Chip for Pulse-Immunoassay....Pages 121-123
Fabrication of on-Chip Sorter Devices with Sub-Micrometer Scale Channels and Self-Aligned Microelectrodes....Pages 124-126
Powder Handling Device for Drug Formulation....Pages 127-129
Thermal Micropump Using Surface Tension Imbalance in Microchannels....Pages 130-132
Optimizatiion of Electrokinetic Pumps for Chip-Based Chromatographic Separations....Pages 133-135
Practical Valves and Pumps for Large-Scale Integration into Microfluidic Analysis Devices....Pages 136-138
Electro Osmotic Flow Pump Based on the Corbino Disc Geometry....Pages 139-141
A Temperature Controlled Micro Valve for Biomedical Applications Using a Temperature Sensitive Hydrogel....Pages 142-144
Electroosmotically Driven Two-Liquid Viscous Pump for Nonconducting Liquids....Pages 145-147
BI-Directional Valve-Less Micropump Using Piezoelectric Micro-Array Actuators....Pages 148-150
Gravity-Driven Micropump with a Steady Flow Rate....Pages 151-153
A micro pump, compatible with multiple manufacturing methods, for fluidhandling in disposable microsystems....Pages 154-156
Microfabrication Process for High-density Micro Pipette Array and Matching Multi-Well Plate with Mixers....Pages 157-159
Microfabricated Phase Change Latched Valve for Integrated Devices....Pages 160-162
Thermal-Actuated Paraffin Microvalves....Pages 163-165
Injection Modes for Evaporation Driven Continuous Liquid Flow....Pages 166-168
DNA Amplification and Hybridization Assays in Integrated Plastic Monolithic Devices....Pages 169-171
Microfluidic Device for Nucleic Acid Fragmentation by Shear Force....Pages 172-174
Combined Nucleic Acid Extraction and Enrichment in Bead-Packed Plastic Beds....Pages 175-177
Measuring Lithium in Whole Blood Using Capillary Electrophoresis....Pages 178-180
An Ingestible Electronic Pill for Real Time Analytical Measurements of the Gastro-Intestinal Tract....Pages 181-183
Detection of Multiple Real-Time Nasba in Nanoliter Volume....Pages 184-186
Disposable Biochip Cartridge for Clinical Diagnostics Toward Point-of-Care Systems....Pages 187-189
Microchip-Based Enzyme-Linked Immunosorbent Assay (ELISA) System....Pages 190-192
Infrared-Mediated Thermocycling for DNA Amplification and Electrophoretic Separation on an Integrated Microchip Device....Pages 193-194
Integrated Microdevice for DNA Extraction from a Single Hair....Pages 195-197
Monolithic Sol-gel Microchip Device for Efficient Isolation of Nucleic Acid From Clinical Samples....Pages 198-200
Miniaturization of a Hematology Analyzer for Monitoring Oncology Outpatients....Pages 201-203
Fabrications and Applications of Micro Hemocytometer for Early Detection of Cancer....Pages 204-206
Enabling Technologies for a Personal Flow Cytometer, Part II: Integrated Analysis Cartridges....Pages 207-209
High Throughput Stress Marker Assay using Polymer Microchip Electrophoresis with Laser induced fluorescence Detection....Pages 210-211
DNA Size Separation Employing Micro-Fabricated Monolithic Nano-Structure....Pages 212-214
Integrated Microsystem of Isothermal Amplification of DNA and Electrophoresis on a Microfabricated Plastic Chip for Detection of Specific Gene and Analysis of Genetic Materials....Pages 215-217
SNP Analysis by Allele-Specific Extension of Fluorescently Labeled Nucleotides in a Microfluidic Flow-Through Device....Pages 218-220
Device for Label-Free Bio-Ligand Interaction Studies Based on Time Resolved Fourier Transform Infrared Spectrometry....Pages 221-223
A Disposable Passive Microfluidic System Integrated with Micromixer and DNA Purification Chip for DNA Sample Preparation....Pages 224-226
DNA hybridization feature on “Bead-array” — DNA probes on beads arrayed in a capillary....Pages 227-229
Development of a Label-Free Protein Array Chip....Pages 230-232
Detection of DNA Point Mutations in a Polymer Microfluidic Network Using a Temporal Thermal Gradient....Pages 233-235
Micro Multiple Immunoassay Using Silicon-Based Microfabricated Protein Biochip....Pages 236-238
An Active Mixer for Microscale Purification and Sequencing Reaction Clean-Up....Pages 239-241
 In Vitro Protein Synthesis on a High-Integrated Microchamber Chip with Low DNA Molecules....Pages 242-244
Parallel Sequencing of Real-World Samples Using A Microfabricated Hybrid Device....Pages 245-247
Micomachinded Device for Manipulation of Charged Bio-Molecules....Pages 248-250
High Precision Micropreparative Separation System Based on Plastc Microfluidic Module — Capillary Coupling....Pages 251-253
High Fidelity and Low Cost Detection of Multi-Color Fluorescence from Biological Cells in a Micro Integrated Flow Cytometer (MIFC) with Disposable Observation Cell....Pages 254-256
Integrated Amorphous Silicon Photodiode Detector for Microfabricated Capillary Electrophoresis Devices....Pages 257-259
Integration of polymer waveguides for optical detection in biochemical microsystems....Pages 260-262
Real-Time SPR Imaging of Biochemical Reactions in Microfluidic Systems....Pages 263-265
A Miniaturized Glow Discharge Optical Emission Detector for Aqueous Analytes....Pages 266-268
Fiber-Optic Microdevices for Surface Plasmon Resonance Applied to Microfluidic Devices....Pages 269-271
Optical Recovery of Particles on a Chip Toward Cell Sorting and Bead-Bed Detection....Pages 272-274
Integrated On-Chip Absorption and Luminescence Spectroscopy with Polydimethylsiloxane (PDMS) Microfluidic Flow Channels Using Broadband Illumination....Pages 275-277
Development of Liquid/Liquid Optical Waveguide Using a Two Phase Sheath Flow and Its Application to Fluorescent Determination of Rhodamine B....Pages 278-280
Integration of Photo-Thermal Spectroscopy Detection System on a Chip....Pages 281-283
Integrated Microfluidic Optical Systems ( i MOS) with Led....Pages 284-286
Application of Organic Light Emitting Diode as an On-Chip Light Source for Fluorescence Spectroscopy....Pages 287-289
Micro-Optical Laser Induced Fluorescence Detection on a Miniaturised Flow Cytometry Device....Pages 290-292
Comparison of Different Strategies of Chemiluminescence Detection for Microchip System Fabricated in Poly(Dimethylsiloxane)....Pages 293-295
Fiber-Loop Ring-Down Spectroscopy for Enhanced Detection of Absorption with Limited Path Length....Pages 296-298
Multicomponent Analysis by Submillimeter-Effective-Length Capillary Electrophoresis using Patterned Fluorescence Correlation Spectroscopy....Pages 299-301
Photopolymerized and Photografted Porous Polymer Monoliths for Fabrication of Microfluidic Analytical Systems....Pages 332-334
Engineered Dielectric Microspheres for Use in Microsystems....Pages 335-337
Microvalve Architectures for High-Pressure Hydraulic and Electrokinetic Fluid Control in Microchips....Pages 338-340
Supramolecular and Macromolecular Approaches to Functional Nano Materials....Pages 302-304
Gas-Liquid Flows in Microchemical Systems....Pages 353-355
On-Chip Concentration of Liquid Samples Using an Air-Liquid Two-Phase Flow....Pages 356-358
Two-Phase Flows (Oil/Water): Flow Regimes and Wetting....Pages 359-361
Label-Free Dielectric Detection of DNA Hybridization with Nanogap Junctions....Pages 305-307
Detection of Single Nucleotide Incoporation Using Pyrosequencing in a Microfluidic Device....Pages 308-310
Fast Screening of Single-Nucleotide Polymorphisms Using Chip-Based Temperature Gradient Capillary Electrophoresis....Pages 311-313
Multiplexed Protein Preparation Systems for Proteomics....Pages 344-346
A Novel Miniaturized Protein Preconcentrator Based on Electric Field-Addressable Retention and Release....Pages 347-349
Design Principles, Performance and Perspectives of a Complete Miniaturized Electrophoretic Instrument....Pages 350-352
Molecular Transport through Nanometer Confined Channels....Pages 314-316
DNA Trap-and-Release Element Employing Electric and Hydro Drag Force Fields for On-Chip Pre-Treatment....Pages 317-319
Microchip Eletrophoresis Integrated with Nanofluidic Sample Handling....Pages 320-322
BioPOEMS and Nanogap DNA Junctions....Pages 341-343
Magnetophoretic-Dielectrophoretic Field-Flow Fractionation....Pages 323-325
Free-Flow Magnetophoresis — Separation of Magnetic Microparticles and Agglomerates on Chip....Pages 326-328
Hybrid Type On-Chip Magnetic Particle Separators for Accurate Positioning Magnetic Beads....Pages 329-331
Preparation of Picoliter-Sized Reaction / Analysis Chambers for Droplet-Based Chemical and Biochemical Systems....Pages 362-364
A Micro-Reactor Equipped with Sheath-Flow Injectors for High-Speed Extraction....Pages 365-367
Mass-Production System of Nearly Monodisperse Diameter Gel Particles Using Droplets Formation in a Microchannel....Pages 368-370
A Multiphase Laminar Flow Diffusion Chip with Ion Selective Electrode Detection....Pages 371-373
Micro PIV Measurement of Electroosmotic Flow....Pages 374-376
High-Efficiency Separation in Microfluidic Devices for High-Throughput Screening of Kinases....Pages 377-379
A Multi-Stage Micro-Tangential Flow Filtration Device for µ TAS Applications....Pages 380-382
Microcontact Switching of Single Molecules....Pages 383-385
Simultaneous Hydrodynamic and Electrokinetic Flow Control....Pages 386-388
A Micro-Fluidic Platform for Contactless, Dropletless Dispensing of High-Density Protein Micro-Arrays....Pages 389-391
PDMS Microstructures Integrated with Detection Elements....Pages 392-394
Development of Plastic Microneedles for Transdermal Interfacing Using Injection Molding Techniques....Pages 395-397
A Novel Bonding Technique for Micro Polymer Chip Using Sacrificial Channel and Adhesive Printing....Pages 398-400
Adhesive and Dead Volume Free Interfacing between PDMS Microfluidic Channels....Pages 401-403
High Pressure Thermal Bonding for Sealing of Plastic Microstructures....Pages 404-406
Innovative Laser Machining and Surface Modification for Plastic Microfluidic Chip....Pages 407-409
Liquid Phase 3D Channel Networks....Pages 410-412
Design and Fabrication of Metal Electrodes Implanted PDMS Structures for Micro Flow Device....Pages 413-415
Strong Bonding of Metal Thin-Film Micropatterns on PDMS Plate Using Self-Assembled Monolayers....Pages 416-418
Study on Protein (IgG) Adsorption in Terms of Surface Modification of Cyclic Olefin Copolymer (COC) for Protein Biochip....Pages 419-421
3-D Microfluidic Networks for Combinatorial Chemistry....Pages 422-424
Effect of Surface Modification on Thermoplastic Fusion Bonding for 3-D Microfluidics....Pages 425-427
Surface Modification of Microgels with N -Alkyl Fatty Acid Layers: Ion Gradient Properties....Pages 428-430
Surface Modification of PDMS for Control of Electroosmotic Flow: Characterization Using Atomic and Chemical Force Microscopy....Pages 431-433
Molecularly-Imprinted Stationary Phases for Plastic Microchip-Based Capillary Electrochomatography....Pages 434-436
Continuous Self Arrangement of Nanoparticles in a Micro-Capillary....Pages 437-439
Surface Modification of Micromolds by Fluorocarbon Films....Pages 440-442
Functionalized Parylene Coatings for Microfluidic Applications....Pages 443-445
Photoresist-Free Micropatterning of Polymer Surfaces Used in Microanalytical Devices....Pages 446-448
A Novel In-Device Enzyme Immobilization Method for Biomems, Demonstrated for a Continuous Glucose Monitor....Pages 449-451
Designing Surface Chemistries for a High Density Chemical Microarray....Pages 452-454
Micro Peptide Array Synthesis on a Chip....Pages 455-457
The Integration of Microarray and Microchannel Using Plastic Chip....Pages 458-460
Diagnostic Antigens and Antibodies Patterned by Micro Stamping System....Pages 461-463
SU-8 Structures for Integrated High-Speed Screening....Pages 464-466
Multimode Optical Waveguide Spectrscopy of Aqueous Gold Sol....Pages 467-469
Prism-Free Broadband Coupling Approach for Spectroelectrochemical Characterization of Surface-Immobilized Molecules....Pages 470-472
Smart, Temperature-Responsive Surfaces....Pages 473-475
Electrokinetic Trapping of Proteins in Cast-to-Shape Matrices....Pages 476-478
Lipid Membrane Microarray with Discrete Chambers....Pages 479-481
Multichannel Capillary Electrochromatography PMMA Microdevice with Integrated Pulsed Conductivity Detector....Pages 485-487
Development of Electrochemical Methods for Monitoring of Single Macromolecule Processes....Pages 488-490
Separation and detection of organic acids in a CE microchip with contactless four-electrode conductivity detection....Pages 491-493
Bioanalytical Devices for Highly Selective Measurement of in Vivo Biochemicals....Pages 494-496
A Novel Contactless Impedance Detector With Shallow Insulation Gap....Pages 497-499
Protein Patterning with Programmable Surface Chemistry Chips....Pages 482-484
Micromachined Polymeric Tip as An Electrospray Ionization Source....Pages 500-502
Modified Porous Silicon Surfaces as DIOS-MS Sample Plates....Pages 503-505
PDMS Electrospray Devices Fabricated by PDMS-Diamond-Coated SU-8 Masters....Pages 506-508
Microfluidic Device with Integrated Protein Digestion, Peptide Separation and Nanospray Interface on Poly (Dimethylsiloxane) PDMS Substrate....Pages 509-511
Free Flow Electrophoresis Device Showing Sub-second Isoelectric Focusing for a Peptide....Pages 539-541
Field Testing and New Applications of Gas Phase Miniature Chemical Analysis Systems (µChemlab™)....Pages 521-523
Results from µChemLab™/CB, a Portable System for Detecting Chemical and Biological Warfare Agents....Pages 524-526
Development of Micro Detector for Benzo[a]pyrene Monitoring....Pages 527-529
Development and Evaluation of a Gas Absorption Microchip Using Porous Glass Plate....Pages 530-532
Development and Optimization of Air-Pollutant Monitoring Device Incorporating with Microchip....Pages 533-535
Portable System for Selective Detection of Aromatic VOC Mixture-Gases Using a Microfluidic Device....Pages 536-538
Quantitative Measurement and Control of Electrokinetically Driven Flow in Microspace....Pages 512-514
Controlling Cell Development by Microfluidic Techniques: A Step Towards Whole-Cell Biosensors with Defined Biological Features....Pages 515-517
Protein Dynamics Study Using a Continuous-Flow Microfluidic Mixer....Pages 518-520
A New Application by Microfabricated Free Flow Electrophoresis Module....Pages 542-544
Nano-Fabricated Size Exclusion Chromatograph....Pages 545-547
Pinpoint Injection and Dielectrophoretic Floating of Autonomously Aligned Microtools....Pages 548-550
Rapid Precolumn Reaction and Chiral Separation on an Integrated Microchip....Pages 551-553
Stepwise Gradient of Linear Polymer Matrices in Microchip Electrophoresis for High Resolution Separation of DNA....Pages 554-556
Continuous Cell Preparation Using Traveling Wave Dielectrophoresis....Pages 557-559
A New Plastic CE Chip with Wide Optical Clarity Using Cyclic Olefin Copolymers (COC)....Pages 560-562
Microchip Eletrophoresis of Oligosaccharides from Glycoprotein....Pages 563-565
Diagnosis of Hepatitis C Virus (HCV) Through Affinity Chromatography using Photolabile Magnetic Beads Linked RNA Aptamer on a Chip....Pages 566-568
Continuous Cell Partitioning in Two-Phase Flow System....Pages 569-571
Gene Mutation Assay by Affinity Microchip Electrophoresis Using DNA-Polyacrylamide Conjugate....Pages 572-574
Removal of Sodium Ion and Chiral Analysis Using Crown Ether as a Chiral Selector in Microchip Electrophoresis....Pages 575-577
A Multilayer Microelectrode Array for Particle Separation by Dielectrophoresis....Pages 578-580
Continuous Free-Flow Electrophoresis Separation of Proteins by Microfabricated Chamber with Micromodule Fraction Separator....Pages 581-583
Viral Separations Using a Microfabricated Electrical Splitt System....Pages 584-586
Chip Based Single-Column TITP (Transient Isotachophoresis) Separator....Pages 587-589
Capillary Electrochromatography and Preconcentration of Neutral Compounds on PDMS Microchips....Pages 590-592
A Microconcentrator for Sensors and Chromatography....Pages 593-595
Miniaturization of Protein Separation: Isoelectric Focusing and SDS-Page....Pages 596-598
On-Line Mid-IR (Quantum Cascade Laser and FTIR Spectrometric) Detection in Capillary Based Separation Systems....Pages 599-601
Monolithic Silica Columns for Capillary HPLC....Pages 602-604
Back Matter....Pages 605-629