Methods in Protein Structure Analysis

Front Matter....Pages i-xvii
Front Matter....Pages 1-1
Characterization of Proteins Separated by Gel Electrophoresis at the Primary Structure Level....Pages 3-14
New Strategies in High Sensitivity Characterization of Proteins Separated from 1-D or 2-D Gels....Pages 15-26
High-Speed Chromatographic Separation of Proteins and Peptides for High Sensitivity Microsequence Analysis....Pages 27-38
Pre-Electrophoretic Labelling of Proteins with a Coloured Water Soluble Edman Reagent....Pages 39-45
Front Matter....Pages 47-47
High Sensitivity Analysis of PTH Amino Acids....Pages 49-55
Synthesis, Evaluation and Application of a Panel of Novel Reagents for Stepwise Degradation of Polypeptides....Pages 57-68
Identification and Characterization of Glycosylated Phenylthiohydantoin Amino Acids....Pages 69-80
Deacetylation and Internal Cleavage of Polypeptides for N-Terminal Sequence Analysis....Pages 81-86
Front Matter....Pages 87-87
A C-Terminal Sequencing Method Using Perfluoroacyl Anhydrides Vapor....Pages 89-107
Sequencing of Proteins from the C-Terminus....Pages 109-118
C-Terminal Protein Sequence Analysis Using the Hewlett-Packard G1009A C-Terminal Protein Sequencing System....Pages 119-129
Automated C-Terminal Sequencing of Polypeptides Containing C-Terminal Proline....Pages 131-138
Front Matter....Pages 139-139
Tandem Mass Spectrometric Characterization of Modified Peptides and Proteins....Pages 141-150
Direct Mass Spectrometric Analyses for Protein Chemistry Studies....Pages 151-159
Peptide-Mass Fingerprinting as a Tool for the Rapid Identification and Mapping of Cellular Proteins....Pages 161-173
The Use of MALDITOF Mass Spectrometry and Amino Acid Sequence Analysis in Characterising Small Amounts of N-Blocked Protein....Pages 175-183
Front Matter....Pages 185-185
Tracing Cell Signalling Pathways Using a Combination of 2D Gel Electrophoresis and Mass Spectrometry....Pages 187-193
Novel Fluorescent Methods for Quantitative Determination of Monosaccharides and Sialic Acids in Glycoproteins by Reveresed Phase High Performance Liquid Chromatography....Pages 195-206
Identification of the Disulfide Bonds of Human Complement Component C9....Pages 207-215
Localization of In Vivo Phosphorylation Sites in Multiphosphorylated Proteins....Pages 217-225
Front Matter....Pages 185-185
Identification and Characterization of Transducin Functional Cysteines, Lysines, and Acidic Residues by Group-Specific Labeling and Chemical Cross-Linking....Pages 227-250
X-Ray Photoelectron Spectroscopy of Amino Acids, Polypeptides and Simple Carbohydrates....Pages 251-260
Measurement of Asp/Asn Damage in Aging Proteins, Chemical Interconversion of Aspartyl Isomers, 18 O Tagging of Enzymatically Repaired Aspartyl Sites, and Enzyme Automethylation at Sites of Asp/Asn Damage....Pages 261-273
Purification Scheme for Isolation and Identification of Peptides Cross-Linked to the rRNA in Ribosomes....Pages 275-282
Investigation of Protein-Protein Interactions in Mitochondrial Steroid Hydroxylase Systems Using Site-Directed Mutagenesis....Pages 283-293
Modification of Chromatin Structure Following Exposure of MOLT4 Cells to the Carcinogenic Chromium(VI)....Pages 295-307
Front Matter....Pages 309-309
Regions of Interaction between Nicotinic Acetylcholine Receptor and α-Neurotoxins and Development of a Synthetic Vaccine Against Toxin Poisoning....Pages 311-326
Immunological Approach to Study the Structure of Oxidized Low Density Lipoproteins....Pages 327-334
Synthetic Combinatorial Libraries: A New Tool for Drug Design....Pages 335-342
DNA-Protein Interactions and Protein-Protein Interactions in Filamentous Bacteriophage Assembly....Pages 343-351
Front Matter....Pages 353-353
Post-Translational Modification by Covalent Phosphorylation of Human Apolipoprotein B-100....Pages 355-367
Mixed Lineage Kinases....Pages 369-380
Agonist-Induced Internalization and Degradation of γ-Aminobutyric Acid A (GABA A ) Receptor Polypeptides from the Neuronal Surface....Pages 381-388
Function and Structure of Human Leucocyte Collagenase....Pages 389-394
The Human DNA-Activated Protein Kinase, DNA-PK: Substrate Specificity....Pages 395-406
The P53 Tumor Suppressor Protein....Pages 407-418
Distinctive Class Relationships Within Vertebrate Alcohol Dehydrogenases....Pages 419-427
Primary Structure of a Novel Stylar RNase Unassociated with Self-Incompatibility in a Tobacco Plant, Nicotiana Alata ....Pages 429-434
Human Fibrinogen Occurs as Over 1 Million Non-Identical Molecules....Pages 435-443
Microsequencing of Proteins of the Rough Endoplasmic Reticulum (rER) Membrane....Pages 445-453
Front Matter....Pages 455-455
Theoretical Studies of Protein Folding....Pages 457-464
Protein Sequence Analysis, Storage and Retrieval....Pages 465-472
Superfamily and Domain....Pages 473-481
Mutational Analysis of the BPTI Folding Pathway....Pages 483-492
Three- and Four-Dimensional Heteronuclear NMR....Pages 493-503
Protein Docking in the Absence of Detailed Molecular Structures....Pages 505-514
A Metric Measure for Comparing Sequence Alignments....Pages 515-525
Back Matter....Pages 527-534