Methods in Protein Sequence Analysis

Front Matter....Pages i-xxvii
Front Matter....Pages xxvii-xxvii
An Anecdotal Account of the History of Peptide Stepwise Degradation Procedures....Pages 1-24
Front Matter....Pages 25-25
An Evaluation of the Current Status of Protein Sequencing....Pages 27-63
Front Matter....Pages 65-65
Strategic Approaches to Sequence Analysis....Pages 67-73
Front Matter....Pages 75-75
A New Protein Microsequenator Using Gas Phase Edman Reagents....Pages 77-90
Towards Longer Degradations on a Sequenator....Pages 91-99
The Nature of Non-Specific Peptide Bond Cleavage During the Isothiocyanate Degradation of Proteins....Pages 101-110
Front Matter....Pages 111-111
Isolation and Sequence Determination of Polypeptides at the Picomole Level....Pages 113-118
Methodologies in the Microsequencing....Pages 119-130
Micro-Sequence Analysis of Ovine Hypothalamic Corticotropin Releasing Factor....Pages 131-138
Identification of Internal Residues of Lactose Permease of Escherichia Coli by Radiolabel Sequencing of Peptide Mixtures....Pages 139-148
The Application of Monoclonal Antibodies to the Microsequencing of Proteins....Pages 149-156
Front Matter....Pages 157-157
Sensitivity Enhancement in Solid-Phase Protein Sequence Analysis: An Examination of Methodology....Pages 159-171
Progress in Solid-Phase Sequencing: Extended Degradations of Peptides and Proteins....Pages 173-180
The use of Dabitc in Automated Solid-Phase Sequencing....Pages 181-188
Improved Automatic Conversion for Use with a Liquid-Phase Sequenator....Pages 189-203
A Study of the Edman Degradation in the Assessment of the Purity of Synthetic Peptides....Pages 205-213
Solid Phase Synthesis of Polypeptides and Oligonucleotides Using a Solid Phase Sequencer....Pages 215-219
Front Matter....Pages 221-221
Manual Batchwise Sequencing Methods....Pages 223-232
Micro Quantitative Edman Manual Sequencing....Pages 233-239
Front Matter....Pages 241-241
Protein Sequencing by Mass Spectrometry....Pages 243-261
Front Matter....Pages 241-241
Sequence Analysis of Polypeptides by Direct Chemical Ionization Mass Spectrometry....Pages 263-270
A New Computer-Aided Method for Sequencing a Polypeptide from the Masses and Edman-Degradation of its Constituent Peptide Fragments....Pages 271-278
The Complementarity of Mass Spectrometry to Edman Degradation or DNA Based Protein Sequencing....Pages 279-288
Front Matter....Pages 289-289
Enzymic Cleavage of Proteins....Pages 291-304
Unusual Enzymatic Cleavage and Preparative Peptide Separation by High Pressure Liquid Chromatography in the Establishment of the Amino Acid Sequence of Tubulin....Pages 305-312
Chemical Cleavage of Proteins....Pages 313-323
Protein Fragmentation with o -Iodosobenzoic Acid....Pages 325-334
Front Matter....Pages 335-335
Analysis of the Escherichia Coli ATP-Synthase Complex by DNA and Protein Sequencing....Pages 337-354
The Primary Structure of Escherichia Coli RNA Polymerase. Nucleotide Sequences of the rpoB and rpoC Genes and Amino Acid Sequences of the β and β’ Subunits....Pages 355-362
Correlation Between DNA and Protein Sequences in β-Galactosidase Related Proteins....Pages 363-371
The Molecular Consequences of Formaldehyde and Ethyl Methanesulfonate Mutagenesis in Drosophila : Analysis of Mutants in the Alcohol Dehydrogenase Gene....Pages 373-379
Front Matter....Pages 381-381
Protein Microsequencing by HPLC Peptide Purification and Solid Phase Edman Degradation....Pages 383-399
Reverse Phase High-Performance Liquid Chromatography for Protein Purification: Insulin-Like Growth Factors, Ca 2+ - Binding Proteins and Metallothioneins....Pages 401-408
Reversed Phase (RP) HPLC of Proteins and Peptides and Its Impact on Protein Microsequencing....Pages 409-416
Preparative Isolation of Ia Antigen Membrane Protein Component Polypeptides on C 18 Reverse Phase HPLC....Pages 417-426
Chromatography and Rechromatography in HPLC Separation of Peptides....Pages 427-434
Sequence Analysis of Membrane Protein Fragments Isolated by High Pressure Liquid Chromatography....Pages 435-446
Preparation of Peptides and Proteins for Sequence Analysis at the Low Nanomole to Subnanomole Level by Reverse-Phase High-Performance Liquid Chromatography: Results for Cytochromes P450 and Fibronectin....Pages 447-454
Analytical and Preparative Applications of High-Performance Liquid Chromatography. Comparisons with Other Methods....Pages 455-462
Separation of Hinge Glycopeptides of Human IgD by HPLC....Pages 463-470
Front Matter....Pages 381-381
Use of High Performance Liquid Chromatography in Characterizing Nucleotide Binding Sites and Antigenic Determinants in cAMP-Dependent Protein Kinase....Pages 471-478
The Use of Radially-Compressed Reversed Phase HPLC for the Separation of Tryptic Peptides of Apolipoproteins....Pages 479-488
Routine Peptide Mapping by High Performance Liquid Chromatography....Pages 489-497
Comparative Peptide Mapping by HPLC: Identification of Single Amino Acid Substitutions in Temperature Sensitive Mutants....Pages 499-507
A New Low Cost, Fully Automated Amino Acid Analyzer Using a Gradient HPLC....Pages 509-515
Front Matter....Pages 517-517
Fused Silica-Capillary-Gas Chromatography. A Novel Procedure for the Identification of PTH in the Analysis of Protein....Pages 519-527
Front Matter....Pages 529-529
Strategies of Sequence Analysis of Regulatory Subunit of Bovine Cardiac Muscle cAMP-Dependent Protein Kinase Type II....Pages 531-532
Enhancement of Sensitivity and Reliability of Automated Sequence Analysis....Pages 533-534
Manual Sequence Analysis of Retinoid-Binding Proteins....Pages 535-536
The Use of H.P.L.C. for Structural Studies on Collagen....Pages 537-537
NH 2 -Terminal Sequence of the Clostripain and NBS Peptides of Streptococcal M5 Protein Purified in One Step by HPLC....Pages 539-540
High-Speed Liquid Chromatography of PTH-Amino Acids....Pages 541-542
Front Matter....Pages 543-543
NADP-Dependent 6-Phosphogluconate Dehydrogenase Stucture of the Enzyme from Sheep Liver....Pages 545-547
Sequence Studies on Na,K-ATPase....Pages 549-550
N-Terminal Amino Acid Sequences of γ-Gliadins from Bread Wheats....Pages 551-551
Recognition of Patterns in Protein Sequences by Metric Analysis....Pages 553-554
Amino Acid Sequence of Bovine β 2 -Microglobulin....Pages 555-556
Sequence Analyses in Characterizations of Functional Properties....Pages 557-558
The Primary Structure Determination of Ribosomal Proteins by Combined Amino Acid and Nucleotide Sequencing....Pages 559-561
Two Different Species of Cytochrome b 5 in One Cell....Pages 563-564
Front Matter....Pages 543-543
A Hydrophobic Tryptic Peptide from Bovine Brain....Pages 565-566
Comparison of N-Terminal Amino Acid Sequences of ω-Gliadins and a Related Prolamin....Pages 567-567
Frog Heart Monomeric Hemoglobin....Pages 569-570
Purification and Micro Sequence Analysis of Active Peptides from Amphibian Skins....Pages 571-572
The Amino Acid Sequence of a Novel Decapeptide from the Skin of the Neotropical Frog Phyllomedusa Sauvagei....Pages 573-574
Structural and Functional Studies on Nucleolar Protein C23 Using Limited Chemical Cleavage and HPLC....Pages 575-576
Function of NAD at the Active Site of Urocanase....Pages 577-578
Amino Acid Sequence of the Alpha and Beta Subunits of Ovine Pituitary Follitropin (FSH)....Pages 579-580
Amino Acid Sequence Diversity Amongst Cytochromes C-556 in the Genus Agrobacterium....Pages 581-582
Back Matter....Pages 583-589