Methods in Protein Sequence Analysis

Front Matter....Pages i-xvi
Front Matter....Pages 1-1
Capillary Liquid Chromatography: A Tool for Protein Structural Analysis....Pages 3-10
Routine Amino Acid Sequencing on 2D-Gel Separated Proteins: A Protein Elution and Concentration Gel System....Pages 11-19
Capillary Electrophoresis in Structural Characterization of Polypeptides....Pages 21-28
Sensitization of Gas-Phase Protein Sequencer using Fluorescein Isothiocyanate (FITC)....Pages 29-36
Thiobenzoylation Method of Protein Sequencing: Gas Chromatography/Mass Spectrometric Detection of 5-Acetoxy-2-Phenylthiazoles....Pages 37-44
Deblocking and Subsequent Microsequence Analysis of N -Terminally Blocked Proteins Immobilized on PVDF Membrane....Pages 45-53
Development of Novel C-Terminal Sequencing Methods....Pages 55-62
Automated C-Terminal Sequencing of Peptides and Proteins....Pages 63-69
A New Chemical Approach to C-Terminal Microsequence Analysis via the Thiohydantoin....Pages 71-78
Cyanogen Bromide Cleavage of Proteins on Blots and Subsequent Separation of the Fragments by Polyacrylamide Gel Electrophoresis Directly from those Blots....Pages 79-85
Identification of the Disulfide Bonds of the Human Complement Component C3....Pages 87-93
A Novel Protease from Jack-Bean Seeds: Asparaginyl Endopeptidase....Pages 95-100
Lysine-Specific Serine Protease from Achromobacter Lyticus : Its Substrate Specificity and Comparison with Trypsin....Pages 101-106
Structure and Function of Snake Venom Metalloproteinase Family....Pages 107-115
Front Matter....Pages 117-117
Recent Advances in Protein Sequencing by Mass Spectrometry Introduction and Overview....Pages 119-126
Sequence Analysis of Peptides Presented to the Immune System by Class I and Class II MHC Molecules....Pages 127-133
High Precision Mass Spectrometry with the Ion Trap Mass Spectrometer....Pages 135-141
Electrospray-Mass Spectrometry, an Emerging Methodology for Elucidating Structure-Function Relationships of Proteins....Pages 143-147
A General Strategy for the use of Mass Spectrometric Molecular Weight Information in Protein Purification and Sequence Determination....Pages 149-156
Determination of Posttranslational Modifications by Mass Spectrometry....Pages 157-164
Front Matter....Pages 165-165
Post-Translational Modifications of Proteins....Pages 167-172
N-Terminal Acetylation of Mutationally Altered Form of Iso-1-Cytochromes c in Normal and nat1 - Strains Deficient in the Major N-Terminal Acetyl Transferase of the Yeast Saccharomyces Cerevisiae ....Pages 173-181
Posttranslational Glutamylation of Several Brain Tubulin Isotypes: Structure of the Polyglutamyl Side-Chain....Pages 183-190
Protein Anchoring to Membrane by Glycosylphosphatidylinositol. Determination of the COOH-Terminal Signal Peptide Sequence and GPI-Attachment Site in Bovine Liver 5′-Nucleotidase....Pages 191-194
Pyridylamination for Sensitive Analysis of Sugar Chains of Glycoproteins....Pages 195-198
Sequence Analysis of Phosphopeptides and its Application for the Determination of Phosphorylated Sites of Proteins....Pages 199-206
Front Matter....Pages 207-207
Prediction of Protein Structure from Multiple Sequence Alignment....Pages 209-220
Sequence Patterns that Characterize Protein Families with a Common Fold....Pages 221-227
Domains and Modules of Proteins....Pages 229-232
Homology Search and Prediction of Biological Function of Protein from Amino Acid Sequences....Pages 233-240
Amino Acid Sequence Comparison as an Aid to Determining Evolutionary Origins....Pages 241-246
Protein Databases Constructed by Quantitative Two-Dimensional Gel Electrophoresis....Pages 247-253
Front Matter....Pages 255-255
Biochemistry of a Natriuretic Peptide Family....Pages 257-260
Activin, Activin-Binding Protein (Follistatin) and Activin Receptor....Pages 261-268
Do Well-Populated Intermediates Represent the Pathway of Protein Folding?....Pages 269-273
Alcohol Dehydrogenases: Patterns of Protein Evolution....Pages 275-282
Structure and Post-Translational Modification of the Lipoyl Domain of 2-Oxo Acid Dehydrogenase Complexes: A New Family of Protein Domains....Pages 283-288
Structure Analysis of the Topography and Molecular Organization of Protein-RNA Complexes as Revealed in Ribosomes....Pages 289-306
Back Matter....Pages 307-310