Methods in Plant Molecular Biology and Biotechnology

1. The Use of Recombinant DNA Technology to Produce Genetically Modified Plants  2. Production and Analysis of Plant Mutants, Emphasizing Arabidopsis thaliana  3. DNA Sequence Organization and Gene Copy Number Determination  4. Isolation and Characterization of Plant DNAs  5. Isolation and Characterization of Plant mRNA  6. Procedures for Introducing Foreign DNA into Plants  7. Vectors for Plant Transformation  8. Construction of l Clone Banks  9. Transient Analysis of Gene Expression in Plant Cells  10. Techniques for Isolating and Characterizing Plant Transcription Promoters, Enhancers, and Terminators  11. Use of Xenopus Oocytes to Monitor Plant Gene Expression  12. In Situ Localization of Specific mRNAs in Plant Tissues  13. Immunological Methods for Assessing Protein Expression in Plants  14. In Situ Immunocytochemical Localization of Plant Proteins  15. Accessing Computer Software for Molecular Biology  16. Sequence Similarity Searches, Multiple Sequence Alignments and Molecular Tree Building  17. DNA Mapping in Plants  18. Random Amplified Polymorphic DNA -RAPD Analysis  19. Detection and Characterization of Plant Pathogens  20. Screening for Inoculant-Quality Strains of Rhizobia  21. Isolation and Characterization of Plant Growth-Promoting Rhizobacteria