Methods in Cellular Imaging

Front Matter....Pages i-xiv
Front Matter....Pages 1-1
Basics of Fluorescence....Pages 5-19
Fluorophores and Their Labeling Procedures for Monitoring Various Biological Signals....Pages 20-39
Detectors for Fluorescence Microscopy....Pages 40-52
Basics of a Light Microscopy Imaging System and Its Application in Biology....Pages 53-65
Laser Scanning Confocal Microscopy Applied to Living Cells and Tissues....Pages 66-87
Functional Imaging of Mitochondria Within Cells....Pages 88-111
Diffusion Measurements by Fluorescence Recovery After Photobleaching....Pages 112-127
Processing Microscope-Acquired Images for Use in Multimedia, Print, and the World Wide Web....Pages 128-142
Front Matter....Pages 143-143
Basic Principles of Multiphoton Excitation Microscopy....Pages 147-161
Building a Two-Photon Microscope Using a Laser Scanning Confocal Architecture....Pages 162-179
Two-Photon Microscopy in Highly Scattering Tissue....Pages 180-199
Multiphoton Laser Scanning Microscopy and Dynamic Imaging in Embryos....Pages 200-215
In vivo Diffusion Measurements Using Multiphoton Excitation Fluorescence Photobleaching Recovery and Fluorescence Correlation Spectroscopy....Pages 216-235
Cellular Response to Laser Radiation in Fluorescence Microscopes....Pages 236-251
Front Matter....Pages 253-256
Measurement of Fluorescence Resonance Energy Transfer in the Optical Microscope....Pages 257-272
Frequency-Domain Fluorescence Lifetime Imaging Microscopy: A Window on the Biochemical Landscape of the Cell....Pages 273-294
Wide-Field, Confocal, Two-Photon, and Lifetime Resonance Energy Transfer Imaging Microscopy....Pages 295-308
One- and Two-Photon Confocal Fluorescence Lifetime Imaging and Its Applications....Pages 309-323
Biological Applications of Time-Resolved, Pump-Probe Fluorescence Microscopy and Spectroscopy in the Frequency Domain....Pages 324-340
Front Matter....Pages 341-343
Spectral Microscopy for Quantitative Cell and Tissue Imaging....Pages 345-361
Front Matter....Pages 341-343
Total Internal Reflection Fluorescence Microscopy....Pages 362-380
Laser Traps in Cell Biology and Biophysics....Pages 381-394
Bioluminescence Imaging of Gene Expression in Living Cells and Tissues....Pages 395-408
Imaging Living Cells and Mapping Their Surface Molecules with the Atomic Force Microscope....Pages 409-423
Back Matter....Pages 425-434