Methods in Cell Biology, Vol. 49

Content: 
Series Editors
Page ii

Edited by
Page iii

Copyright Page
Page iv

Contributors
Pages xv-xviii

Preface
Pages xix-xx
David W. Galbraith, Hans J. Bohnert, Don P. Bourque

Chapter 1 Advances in High-Pressure and Plunge-Freeze Fixation Original Research Article
Pages 3-19
M.E. Galway, J.W. Heckman Jr., G.J. Hyde, L.C. Fowke

Chapter 2 Ion Localization and X-Ray Microanalysis Original Research Article
Pages 21-31
Martin J. Hodson

Chapter 3 Freeze-Fracture Deep-Etch Methods Original Research Article
Pages 33-43
Chris Hawes, Barry Martin

Chapter 4 Advances in Immunoelectron Microscopy Original Research Article
Pages 45-56
Patricia J. Moore

Chapter 5 Freeze-Substitution Original Research Article
Pages 57-69
M.V. Parthasarathy

Chapter 6 Cell Optical Displacement Assay (CODA)—Measurements of Cytoskeletal Tension in Living Plant Cells with a Laser Optical Trap Original Research Article
Pages 71-84
Melvin Schindler

Chapter 7 Methods in Plant Immunolight Microscopy Original Research Article
Pages 85-107
Roy C. Brown, Betty E. Lemmon

Chapter 8 Confocal Epipolarization Microscopy of Gold Probes in Plant Cells and Protoplasts Original Research Article
Pages 109-121
Lawrence R. Griffing, Marco A. Villanueva, Josephine Taylor, Scott Moon

Chapter 9 Monoclonal Antibodies to Cell-Specific Cell Surface Carbohydrates in Plant Cell Biology and Development Original Research Article
Pages 123-124,124a,124b,124c,124d,124e,124f,124g,124h,124i,124j,125-141
Roger I. Pennell, Keith Roberts

Chapter 10 Epitope Tagging for the Detection of Fusion Protein Expression in Transgenic Plants Original Research Article
Pages 143-151
Mark J. Guiltinan, Lauren McHenry

Chapter 11 In Situ Enzyme Histochemistry on Plastic-Embedded Plant Material Original Research Article
Pages 153-163
Marc De Block

Chapter 12 In Situ Hybridization Original Research Article
Pages 165-183
Geoffrey Ian McFadden

Chapter 13 Localization of RNA by High Resolution in Situ Hybridization Original Research Article
Pages 185-199
Xingxiang Li, Thomas W. Okita

Chapter 14 Recombinant Aequorin Methods for Intracellular Calcium Measurement in Plants Original Research Article
Pages 201-216
Heather Knight, Marc R. Knight

Chapter 15 Confocal Microscopy of the Shoot Apex Original Research Article
Pages 217-229
Mark P. Running, Steven E. Clark, Elliot M. Meyerowitz

Chapter 16 Measurements of Wall Stress Relaxation in Growing Plant Cells Original Research Article
Pages 231-243
Daniel J. Cosgrove

Chapter 17 High-Resolution NMR Methods for Study of Higher Plants Original Research Article
Pages 245-258
Justin K.M. Roberts, Jian-Hua Xia

Chapter 18 Electrophysiology Original Research Article
Pages 259-274
John F. Thain

Chapter 19 Ion-Selective Microelectrodes for Measurement of Intracellular Ion Concentrations Original Research Article
Pages 275-291
A.J. Miller

Chapter 20 Patch-Clamping Plant Cells Original Research Article
Pages 293-302
Frans J.M. Maathuis, Dale Sanders

Chapter 21 Methods for Mesophyll and Bundle Sheath Cell Separation Original Research Article
Pages 305-314
Jen Sheen

Chapter 22 Synchronization Of Cell Cultures Of Higher Plants Original Research Article
Pages 315-329
Hiroaki Kodama, Atsushi Komamine

Chapter 23 Genetic Tagging of Cells and Cell Layers for Studies of Plant Development Original Research Article
Pages 331-354
Angelo Spena, Francesco Salamini

Chapter 24 Chemically Induced Mitotic Synchrony in Root Apical Meristems Original Research Article
Pages 355-365
Betty Prewett, Thomas Jacobs

Chapter 25 Manipulation of Pollen Grains for Gametophytic and Sporophytic Types of Growth Original Research Article
Pages 367-375
V. Raghavan

Chapter 26 Root Border Cells as Tools in Plant Cell Studies Original Research Article
Pages 377-387
Lindy A. Brigham, Ho-Hyung Woo, Martha C. Hawes

Chapter 27 In Vivo Footprinting of Protein—DNA Interactions Original Research Article
Pages 391-400
Anna-Lisa Paul, Robert J. Ferl

Chapter 28 The Interaction Trap: In Vivo Analysis of Protein—Protein Associations Original Research Article
Pages 401-416
Brenda W. Shirley, Inhwan Hwang

Chapter 29 Cloning Plant Genes by Complementation of Yeast Mutants Original Research Article
Pages 417-430
Christophe d'Enfert, Michèle Minet, François Lacroute

Chapter 30 Differential mRNA Display Original Research Article
Pages 431-440
T. Lynne Reuber, Frederick M. Ausubel

Chapter 31 Molecular Methods for Isolation of Signal Transduction Pathway Mutants Original Research Article
Pages 441-454
Joanne Chory, Hsou-min Li, Nobuyoshi Mochizuki

Chapter 32 Induction of Signal Transduction Pathways through Promoter Activation Original Research Article
Pages 455-469
R. Walden, K. Fritze, H. Harling

Chapter 33 In Vitro Analysis of G-Protein Functions Original Research Article
Pages 471-485
Hong Ma, Catherine A. Weiss

Chapter 34 Production of Recombinant Plant Calmodulin and Its Use to Detect Calmodulin-Binding Proteins Original Research Article
Pages 487-500
Birong Liao, Raymond E. Zielinski

Chapter 35 Analysis of the Light Signaling Pathway in Stomatal Guard Cells Original Research Article
Pages 501-513
Ken-Ichiro Shimazaki, Toshinori Kinoshita

Chapter 36 Immunocytochemical Localization of Receptor Protein Kinases in Plants Original Research Article
Pages 515-529
Veronica P. Counihan, Thomas E. Phillips, John C. Walker

Chapter 37 Expression and Assay of Autophosphorylation of Recombinant Protein Kinases Original Research Article
Pages 531-541
Mark A. Horn, John C. Walker

Chapter 38 Transmembrane Signaling and Phosphoinositides Original Research Article
Pages 543-554
Myeon H. Cho, Wendy F. Boss

Index
Pages 555-568

Volumes In Series
Pages 569-573