Methods for Plant Molecular Biology

Content: 
SELECTED METHODS IN ENZYMOLOGY, Page ii
Front Matter, Page iii
Copyright, Page iv
List of Contributors, Pages ix-xi
Preface, Page xiii, ARTHUR WEISSBACH, HERBERT WEISSBACH
Contents of Methods in Enzymology, Volume 118, Pages xv-xix
1 - Isolation and Characterization of Plant Cell Walls and Cell Wall Components, Pages 3-40, WILLIAM S. YORK, ALAN G. DARVILL, MICHAEL MCNEIL, THOMAS T. STEVENSON, PETER ALBERSHEIM
2 - Isolation of the Plasma Membrane, Pages 41-54, THOMAS K. HODGES, DAVID MILLS
3 - Purification and Restriction Endonuclease Analysis of Plant Nuclear DNA, Pages 57-75, JOHN C. WATSON, WILLIAM F. THOMPSON
4 - Analyzing Genome Variation in Plants, Pages 77-87, CAROL RIVIN
5 - The Synthesis of High-Molecular-Weight Proteins in the Wheat Germ Translation System, Pages 91-101, M.D. MORCH, G. DRUGEON, W. ZAGORSKI, A.L. HAENNI
6 - Isolation and Structural Analysis of Chloroplast DNA, Pages 105-124, JEFFREY D. PALMER
7 - In Vitro Transcription of Chloroplast Protein Genes, Pages 125-146, EMIL M. OROZCO JR., JOHN E. MULLET, LINDA HANLEY-BOWDOIN, NAM-HAI CHUA
8 - Coupled Transcription-Translation in Chloroplast Lysates, Pages 147-158, J.D.J. BARD, D.P. BOURQUE, D. ZAITLIN
9 - Translation by Isolated Pea Chloroplasts, Pages 159-172, HELEN T. NIVISON, LEONARD E. FISH, ANDRE T. JAGENDORF
10 - Isolation and Characterization of Chloroplast Envelope Membranes, Pages 173-182, KENNETH KEEGSTRA, ABDULLAH E. YOUSIF
11 - Characterization of the Thylakoid Membrane by Subfractionation Analyses, Pages 183-195, BERTIL ANDERSSON
12 - Subunit Structure and Biogenesis of ATP Synthase and Photosystem I Reaction Center, Pages 197-214, NATHAN NELSON
13 - Cloning and Expression of Genes for the Small Subunit of Ribulosebisphosphate Carboxylase, Pages 215-228, C.F. WIMPEE, E.M. TOBIN
14 - Separation and Reassembly of the Subunits of Ribulosebisphosphate Carboxylase, Pages 229-237, T. JOHN ANDREWS
15 - The Cloning and Expression in Escherichia coli of RuBP Carboxylase/Oxygenase Large Subunit Genes, Pages 239-253, C.R. SOMERVILLE, L. MCINTOSH, J. FITCHEN, M. GUREVITZ
16 - The Isolation of Mitochondria and Mitochondrial DNA, Pages 257-273, MAUREEN R. HANSON, MAURY L. BOESHORE, PHILLIP E. MCCLEAN, MARY A. O'CONNELL, HELEN T. NIVISON
17 - Analysis of the Genome Structure of Plant Mitochondria, Pages 275-292, DAVID M. LONSDALE, TONY P. HODGE, PETER J. STOEHR
18 - Strategies for the Identification and Analysis of Higher Plant Mitochondrial Genes, Pages 293-307, ADAM J. DAWSON, VALERIE P. JONES, CHRISTOPHER J. LEAVER
19 - Isolation of Plant Mitochondrial RNA, Pages 309-317, DAVID B. STERN, KATHLEEN J. NEWTON
20 - Isolation and Characterization of Nitrogenase from Klebsiella pneumoniae, Pages 321-329, VINOD K. SHAH
21 - Tn5 Mapping of Rhizobium Nitrogen Fixation Genes, Pages 331-340, G. DITTA
22 - Establishment of Calli and Suspension Cultures, Pages 343-353, ROBERTA H. SMITH
23 - Protoplasts: Isolation, Culture, Plant Regeneration, Pages 355-383, INGO POTRYKUS, RAYMOND D. SHILLITO
24 - Fusion and Transformation of Plant Protoplasts, Pages 385-401, J.B. POWER, M.R. DAVEY, J.P. FREEMAN, B.J. MULLIGAN, E.C. COCKING
25 - Organelle Transfer, Pages 403-419, ESRA GALUN, DVORA AVIV
26 - Gene Transfer in Plants: Production of Transformed Plants Using Ti Plasmid Vectors, Pages 423-436, STEPHEN G. ROGERS, ROBERT B. HORSCH, ROBERT T. FRALEY
27 - Plant Virus Vectors: Cauliflower Mosaic Virus, Pages 437-446, N. BRISSON, T. HOHN
28 - Direct Gene Transfer to Plants, Pages 447-463, JERZY PASZKOWSKI, MICHAEL W. SAUL
29 - In Vitro Transcription of Infectious Viral RNA from Cloned cDNA, Pages 467-479, PAUL AHLQUIST
30 - Use of RNA Probes to Detect Plant RNA Viruses, Pages 481-486, S.J. GARGER, T.H. TURPEN
31 - Preparation and Use of cDNA Probes for Detection of Viral Genomes, Pages 487-506, PETER PALUKAITIS
32 - ELISA Techniques, Pages 507-530, MICHAEL F. CLARK, RICHARD M. LISTER, MOSHE BAR-JOSEPH
Subject Index, Pages 531-543