Measuring Immunity: Basic Science and Clinical Practice

Cover Page......Page 1
Dedication......Page 3
Inside Cover......Page 4
Copyright © 2005......Page 5
Contents......Page 6
Foreword......Page 10
Preface......Page 14
Contributors......Page 16
Section I - Fundamentals of the immune response......Page 22
1. MHC Class I......Page 24
2. MHC Class II......Page 33
3. Cytokine Receptor Heterogeneity......Page 44
4. Genetic Diversity at Human Cytokine Loci in Health and Disease......Page 56
5. Signaling Molecules Affecting Immune Response......Page 83
6. Toll-like Receptors in Innate Immunity......Page 101
7. DNA Sequence-Specific Transcription Factors......Page 112
8. Genetic Diversity in NK and NKT Cells......Page 131
Section II - Serologic assays......Page 140
9. Handling Sera and Obtaining Fluid from Different Compartments: Practical Considerations......Page 142
10. Acute-Phase Proteins and Inflammation: Immunological and Clinical Implications......Page 152
11. Complement in Health and Disease......Page 165
12. Immunoglobulin Titers and Immunoglobulin Subtypes......Page 179
13. Human Antiglobulin Responses......Page 193
14. Rheumatoid Factors......Page 208
15. Autoantibodies......Page 214
16. Antibody Affinity Using Fluorescence......Page 222
17. SLE-Associated Tests......Page 231
18. Multiplexed Serum Assays......Page 242
Section III - Cellular enumeration and phenotyping......Page 252
19. Handling and Storage of Cells and Sera: Practical Considerations......Page 254
20. Phenotypic and Functional Measurements on Circulating Immune Cells and their Subsets......Page 258
21. Natural Killer Cells......Page 278
22. Tetramer Analysis......Page 289
23. Peripheral Blood Naive and Memory B Cells......Page 298
24. Dendritic Cells......Page 311
25. Monocytes and Macrophages......Page 320
26. Tumor Cells......Page 333
27. Regulatory T. Cells......Page 343
28. Intracellular Cytokine Assays......Page 357
Section IV - Cellular function and physiology......Page 362
29. Cytolytic Assays......Page 364
30. Mixed Leukocyte Reactions......Page 371
31. Antigen/Mitogen-Stimulated Lymphocyte Proliferation......Page 382
32. Monitoring Cell Death......Page 390
33. Cytokine Enzyme Linked Immunosorbent Spot (ELISPOT) Assay......Page 401
34. Testing Natural Killer Cells......Page 417
Section V - Provocative assays in vivo......Page 426
35. Delayed Type Hypersensitivity Responses......Page 428
36. Rebuck Windows: Granulocyte Function......Page 440
37. The Vascular and Coagulation Systems......Page 449
38. Sentinel Node Assays......Page 455
39. Imaging Inflammation......Page 466
Section VI - Assays in Acute and Chronic Diseases......Page 484
40. Cancer – Solid Tumors......Page 486
41. Cancer – Hematologic Disorders......Page 494
42. Autoimmunity – RheumatoidArthritis......Page 502
43. Autoimmunity – Type 1 Diabetes......Page 515
44. Autoimmunity – Systemic Lupus Erythematosus......Page 526
45. Autoimmunity – Multiple Sclerosis......Page 536
46. Autoimmunity – Inflammatory Bowel Disease......Page 546
47. Autoimmunity – Endocrine......Page 564
48. Autoimmunity – Vasculitis......Page 581
49. Transplantation......Page 590
50. Viral Responses – HIV-1......Page 599
51. Viral Responses – Epstein-Barr Virus......Page 608
52. Viral Responses – Hepatitis......Page 619
53. Dermatology......Page 631
54. Arteriosclerosis......Page 641
55. Primary Immunodeficiencies......Page 651
56. Asthma and Allergy......Page 660
Section VII - New technologies......Page 668
57. Serum Proteomic Profiling and Analysis......Page 670
58. Imaging Cytometry: High Content Screening for Large-Scale Cell Research......Page 681
59. Cancer Biometrics......Page 687
60. Genomics and Microarrays......Page 718
61. Image Informatics......Page 728
Index......Page 734