Manual of Research Techniques in Cardiovascular Medicine

Content: Cover
 Dedication
 Title page
 Copyright page
 Contents
 List of Contributors
 Preface
 About the Companion Website
 Part 1: Electrophysiology
 1: Measurement of calcium transient ex vivo
 Introduction
 Preparation and fluorescent indicator loading
 Optical setup
 Calibration
 Analysis and interpretation
 Set-by-step procedure
 References
 2: Confocal imaging of intracellular calcium cycling in isolated cardiac myocytes
 Introduction
 Materials and methods
 Chamber, electrical stimulation, and superfusate delivery apparatus
 Confocal microscope system. Cell loading of fluorescent Ca2+ indicatorsExperimental approach
 Data analyses
 Weaknesses and strengths of the method and alternative approaches
 Conclusions
 References
 3: Generating a large animal model of persistent atrial fibrillation
 Introduction
 Insights from tachypacing-induced AF models
 Protocol
 Generating a tachypacing-induced AF model
 Pacing protocols
 Ventricular rate during AF: to ablate or not to ablate
 Strengths and weaknesses of the model
 Complications and alternative approaches to study AF
 Potential complications
 Alternative AF models
 Conclusion
 Acknowledgement. Choices to make before an experimentAP recording procedure
 Frequent problems
 AP analysis
 Alternative approaches and extensions
 Weaknesses and strengths
 Conclusion
 References
 6: Patch-clamp recordings from isolated cardiac myocytes
 Introduction
 Theory of patch-clamp recording
 Cell-attached configuration
 Whole-cell recording configuration
 Inside-out excised patch configuration
 Outside-out excised patch configuration
 Instrumentation for patch-clamp recordings
 Experimental aspects of patch-clamp recordings
 Gigaseal formation
 Whole-cell recordings. Whole-cell voltage-clamp protocols, data acquisition, and analysisConclusions and future directions
 References
 7: Optical mapping of the heart
 Introduction
 Protocols
 Experimental set-up
 Tissue preparations (Figure 7.2)
 Staining and image acquisition
 Data processing and analysis
 Representative results
 Alternative approaches
 Weaknesses and strengths of method
 Conclusions
 References
 Part 2: Isolation and Maintenance of Primary Stem Cells
 8: Isolation of colony-forming endothelial progenitor cells
 Introduction
 Materials
 Laboratory reagents
 Laboratory equipment
 Methods.