Macromolecular Biorecognition: Principles and Methods

Front Matter....Pages i-xix
Front Matter....Pages 1-1
Computer Graphics as an Aid in Protein Structure-Function Analysis and Design....Pages 3-11
Computational Approaches to the Study of Protein — Ligand Interactions....Pages 13-28
Synthetic Peptides and the Design of Peptide and Protein Recognition Surfaces....Pages 29-50
Limited Proteolysis of Globular Proteins: Molecular Aspects Deduced from Studies on Thermolysin....Pages 51-67
Crystallographic Studies on Retinol-Binding Protein and Beta Lactoglobulin....Pages 69-79
Structural Bases for the Recognition of Inhibitors by Serine Proteinases and their Zymogens....Pages 81-100
Role of the Primary Specificity Subsite on the Interaction between Serine Proteinases and Low Molecular Weight Substrates and Inhibitors....Pages 101-115
Interaction between Serine (Pro)Enzymes and Macromolecular Inhibitors. Thermodynamic and Kinetic Aspects....Pages 117-130
Subunit Interactions in Cytochrome Oxidase: The Role of Subunit III....Pages 131-138
Myosin Subfragment 1 Catalyzes the Polymerization of Actin at Low Ionic Strength....Pages 139-142
Structural Effects in the Self-Association of γ II-Crystallin....Pages 143-146
The Role of Lysine-7 in Ribonuclease-A....Pages 147-150
Front Matter....Pages 151-151
Transition of Chromatin from the “10 nm” Lower Order Structure, to the “30 nm” Higher Order Structure, as Followed by Small Angle X-Ray Scattering....Pages 153-168
Topology of DNA in its Interaction with Actinomycin D and with the Histone Octamer....Pages 169-183
Thermodynamics and Stereochemistry of the Interaction between Anthraquinone Drugs and DNA....Pages 185-198
Interaction of cis-Ru(DMSO) 4 Cl 2 with DNA in Aqueous Solution....Pages 199-212
Studies on the Interaction between Ribosome and Elongation Factor 2 by Fluorescent Labeling of the Diphtamide Residue....Pages 213-216
Front Matter....Pages 217-217
Fine Specificity of Monoclonal Anti-Lysozyme Antibodies and the Three-Dimensional Structure of a Lysozyme-Antibody Complex....Pages 219-226
Predicting Antigenicity in Proteins and the Future of Synthetic Peptide Vaccines....Pages 227-233
Folding and Antigenicity of Thermolysin Fragments....Pages 235-250
Front Matter....Pages 217-217
Synthesis and Biological Activity of the Immunomodulatory Site of Interleukin 1....Pages 251-258
The 208–222 Region of the Peplomer Glycoprotein as a Putative Binding Site of Rabies Virus with the Nicotinic Acetylcholine Receptor....Pages 259-272
Heterogeneity and Specificity of Murine Cell Response to Synthetic Peptide (NANP) n of P. Falciparum Circumsporozoite Protein....Pages 273-278
Mapping of Antigen Epitopes Interacting with Class II MHC Products and with the Antigen Receptor of T Lymphocytes....Pages 279-287
Front Matter....Pages 289-289
Analytical Use of Antibodies Based on Specific Molecular Binding....Pages 291-302
Modified Avidins in Detection Systems....Pages 303-307
New Biospecific Chromatographies of β-Hexosaminidases....Pages 309-319
Preparative Applications of Subunit Exchange Chromatography....Pages 321-332
Use of Monospecific Antibodies for the Purification of D-Amino Acid Oxidases from Various Sources....Pages 333-339
Adsorption of Aromatic Molecules on Hydrophilic Gel Chromatography Media. Effect of Various Salts....Pages 341-345
Back Matter....Pages 347-356