Lymphangiogenesis: Methods and Protocols

Front Matter ....Pages i-xii
 Three-Dimensional Visualization of the Lymphatic Vasculature  (Cathrin Dierkes, Aaron Scherzinger, Friedemann Kiefer)....Pages 1-18
 Histological and Morphological Characterization of Developing Dermal Lymphatic Vessels  (Kelly L. Betterman, Natasha L. Harvey)....Pages 19-35
 Genetic Lineage Tracing of Lymphatic Endothelial Cells in Mice  (Ines Martinez-Corral, Taija Makinen)....Pages 37-53
 Visualization and Tools for Analysis of Zebrafish Lymphatic Development  (Kazuhide S. Okuda, Sungmin Baek, Benjamin M. Hogan)....Pages 55-70
 Characterization of Zebrafish Facial Lymphatics  (Tiffany C. Y. Eng, Jonathan W. Astin)....Pages 71-83
 Correlative Fluorescence and Scanning Electron Microscopy to Study Lymphovenous Valve Development  (Xin Geng, R. Sathish Srinivasan)....Pages 85-96
 Characterization of Mouse Mesenteric Lymphatic Valve Structure and Function  (Amélie Sabine, Michael J. Davis, Esther Bovay, Tatiana V. Petrova)....Pages 97-129
 Morphological Analysis of Lacteal Structure in the Small Intestine of Adult Mice  (Sang Heon Suh, Seon Pyo Hong, Intae Park, Joo-Hye Song, Gou Young Koh)....Pages 131-139
 Morphological and Functional Analysis of CNS-Associated Lymphatics  (Jasmin Herz, Antoine Louveau, Sandro Da Mesquita, Jonathan Kipnis)....Pages 141-151
 Morphological Analysis of Schlemm’s Canal in Mice  (Benjamin R. Thomson, Susan E. Quaggin)....Pages 153-160
 Imaging Lymphatics in Mouse Lungs  (Peter Baluk, Donald M. McDonald)....Pages 161-180
 Imaging of Endothelial Cell Dynamic Behavior in Zebrafish  (Baptiste Coxam, Holger Gerhardt)....Pages 181-195
 Visualization and Measurement of Lymphatic Function In Vivo  (Samia B. Bachmann, Michael Detmar, Steven T. Proulx)....Pages 197-211
 Investigating Effects of Fluid Shear Stress on Lymphatic Endothelial Cells  (Daniel T. Sweet, Joshua D. Hall, John Welsh, Mark L. Kahn, Juan M. Jiménez)....Pages 213-227
 Methods for Assessing the Contractile Function of Mouse Lymphatic Vessels Ex Vivo  (Jorge A. Castorena-Gonzalez, Joshua P. Scallan, Michael J. Davis)....Pages 229-248
 Evaluation and Characterization of Endothelial Cell Invasion and Sprouting Behavior  (Jocelynda Salvador, George E. Davis)....Pages 249-259
 Dorsal Ear Skin Window for Intravital Imaging and Functional Analysis of Lymphangiogenesis  (Witold W. Kilarski, Esra Güç, Melody A. Swartz)....Pages 261-277
 Isolation of Human Skin Lymphatic Endothelial Cells and 3D Reconstruction of the Lymphatic Vasculature In Vitro  (Anita Rogic, Francois Auger, Mihaela Skobe)....Pages 279-290
 Stimulation and Inhibition of Lymphangiogenesis Via Adeno-Associated Viral Gene Delivery  (Sinem Karaman, Harri Nurmi, Salli Antila, Kari Alitalo)....Pages 291-300
 Isolation and Characterization of Mouse Organ-Specific Endothelial Transcriptomes  (Shahin Rafii, Brisa Palikuqi)....Pages 301-308
 Single-Cell mRNA Sequencing of the Mouse Brain Vasculature  (Michael Vanlandewijck, Christer Betsholtz)....Pages 309-324
 Metabolic Analysis of Lymphatic Endothelial Cells  (Pengchun Yu, Tiago C. Alves, Richard G. Kibbey, Michael Simons)....Pages 325-334
 Characterizing Epigenetic Changes in Endothelial Cells  (Matthew T. Menendez, Courtney T. Griffin)....Pages 335-344
Back Matter ....Pages 345-345