Lipid-Protein Interactions: Methods and Protocols

Front Matter ....Pages i-xiv
 Multiscale Modeling and Simulation Approaches to Lipid–Protein Interactions  (Roland G. Huber, Timothy S. Carpenter, Namita Dube, Daniel A. Holdbrook, Helgi I. Ingólfsson, William A. Irvine et al.)....Pages 1-30
 Quartz Crystal Microbalances as Tools for Probing Protein–Membrane Interactions  (Søren B. Nielsen, Daniel E. Otzen)....Pages 31-52
 Surface Plasmon Resonance for Measuring Interactions of Proteins with Lipids and Lipid Membranes  (Aleksandra Šakanovič, Vesna Hodnik, Gregor Anderluh)....Pages 53-70
 Thermodynamic Analysis of Protein–Lipid Interactions by Isothermal Titration Calorimetry  (Musti J. Swamy, Rajeshwer S. Sankhala, Bhanu Pratap Singh)....Pages 71-89
 Differential Scanning Calorimetry of Protein–Lipid Interactions  (Olga Cañadas, Cristina Casals)....Pages 91-106
 Imaging and Force Spectroscopy of Single Transmembrane Proteins with the Atomic Force Microscope  (K. Tanuj Sapra)....Pages 107-144
 Kinetics of Insertion and Folding of Outer Membrane Proteins by Gel Electrophoresis  (Andre Schüßler, Sascha Herwig, Jörg H. Kleinschmidt)....Pages 145-162
 Optimized Negative-Staining Protocol for Lipid–Protein Interactions Investigated by Electron Microscopy  (Jianfang Liu, Hao Wu, Changyu Huang, Dongsheng Lei, Meng Zhang, Wei Xie et al.)....Pages 163-173
 Probing Heterogeneous Lipid Interactions with Membrane Proteins Using Mass Spectrometry  (John W. Patrick, Arthur Laganowsky)....Pages 175-190
 Protein Microarrays and Liposome: A Method for Studying Lipid–Protein Interactions  (Samuel Herianto, Chien-Sheng Chen, Heng Zhu)....Pages 191-199
 Structural Investigations of Protein–Lipid Complexes Using Neutron Scattering  (Luke A. Clifton, Stephen C. L. Hall, Najet Mahmoudi, Timothy J. Knowles, Frank Heinrich, Jeremy H. Lakey)....Pages 201-251
 Circular-Dichroism and Synchrotron-Radiation Circular-Dichroism Spectroscopy as Tools to Monitor Protein Structure in a Lipid Environment  (Koichi Matsuo, Kunihiko Gekko)....Pages 253-279
 FTIR Analysis of Proteins and Protein–Membrane Interactions  (Suren A. Tatulian)....Pages 281-325
 UV Resonance Raman Spectroscopy as a Tool to Probe Membrane Protein Structure and Dynamics  (DeeAnn K. Asamoto, Judy E. Kim)....Pages 327-349
 Analyzing Transmembrane Protein and Hydrophobic Helix Topography by Dual Fluorescence Quenching  (Gregory A. Caputo, Erwin London)....Pages 351-368
 Förster Resonance Energy Transfer as a Tool for Quantification of Protein–Lipid Selectivity  (Luís M. S. Loura, Manuel Prieto, Fábio Fernandes)....Pages 369-382
 A Guide to Tracking Single Membrane Proteins and Their Interactions in Supported Lipid Bilayers  (Evan L. Taylor, Kumud Raj Poudel, James A. Brozik)....Pages 383-414
 Fluorescence Correlation Spectroscopy to Examine Protein–Lipid Interactions in Membranes  (Viktoria Betaneli, Jonas Mücksch, Petra Schwille)....Pages 415-447
 Membrane Pore Formation by Peptides Studied by Fluorescence Techniques  (Suren A. Tatulian, Nabin Kandel)....Pages 449-464
 Folding of β-Barrel Membrane Proteins into Lipid Membranes by Site-Directed Fluorescence Spectroscopy  (Lisa Gerlach, Omkolsum Gholami, Nicole Schürmann, Jörg H. Kleinschmidt)....Pages 465-492
 EPR Techniques to Probe Insertion and Conformation of Spin-Labeled Proteins in Lipid Bilayers  (Enrica Bordignon, Svetlana Kucher, Yevhen Polyhach)....Pages 493-528
 Studying Lipid–Protein Interactions with Electron Paramagnetic Resonance Spectroscopy of Spin-Labeled Lipids  (Tibor Páli, Zoltán Kóta)....Pages 529-561
 Solid-State NMR Approaches to Study Protein Structure and Protein–Lipid Interactions  (Christopher Aisenbrey, Evgeniy S. Salnikov, Jesus Raya, Matthias Michalek, Burkhard Bechinger)....Pages 563-598
 Solution NMR Spectroscopy for the Determination of Structures of Membrane Proteins in a Lipid Environment  (Ashish Arora)....Pages 599-643
 Nanodiscs as a New Tool to Examine Lipid–Protein Interactions  (Ilia G. Denisov, Mary A. Schuler, Stephen G. Sligar)....Pages 645-671
Back Matter ....Pages 673-682